Botanical antioxidants

A technology of anti-oxidation and plant extracts, which is used in the field of plant inhibitors as antioxidants and plant inhibitors of DNA damage, and can solve the problems of insufficient stability of antioxidants

Pending Publication Date: 2021-04-02
INNOPHOS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during oxidative stress, antioxidants are not sufficient to maintain homeostasis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 - Preparation of cashew seed coat extract using 70% ethanol solvent

[0056] Dried cashew nut (Anacardium occidentale) seed coat powder (60 g) was filled into three 100 ml stainless steel test tubes, and was heated using a Thermo Scientific TM Dionex TM ASE 350 accelerated solvent extractor or two solvent extractions with 70% ethanol in DI water at a temperature of 80°C and a pressure of 1500 psi. Filter and collect the extract solution. The combined ethanol extract solutions were evaporated under vacuum with a rotary evaporator to give crude cashew seed coat extract.

[0057] The extraction results are provided in Table 1 below.

[0058] Table 1 - Extraction of cashew seed coat

[0059]

Embodiment 2

[0060] Example 2 - Catechin Quantification of Cashew Nut Seed Coat Extract

[0061] A C18 reversed-phase column ( 5μm C18(2) LC column 250x 4.6mm, available from Torrance, California, US), to determine the free catechins present in the cashew seed coat extract. For mobile phase A, the solvent was 0.10% phosphoric acid in water (“H 3 PO 4 "), and for mobile phase B, solvent B was acetonitrile ("ACN"), which was used for elution at a flow rate of 1.0 ml / min, and using UV absorption at 275 nm and a column temperature of 35 °C. Using The catechin reference standard was obtained from Sigma-Aldrich Co. The reference standard was dissolved in methanol ("MeOH"): 0.1% H 3 PO 4 (1:1 ratio), wherein the concentration of catechin (C1251) is 0.5 mg / ml, and the concentration of epicatechin (E1753) is 0.1 mg / ml. Test samples were prepared in volumetric flasks at a concentration of 2 mg / ml in 0.1% H containing 50% MeOH 3 PO 4 , sonicate until dissolved (approximately 10 minutes), ...

Embodiment 3

[0065] Example 3-Chemical analysis of cashew seed coat extract

[0066] Using ultrahigh pressure liquid chromatography ("HPLC") and mass spectrometry ( UPLC Class I and The GS-XT-QTof system, both available from Water Corporation, Milford, Massachusetts USA), determined the flavonoid compounds present in the cashew seed coat extract. The columns used are UPLC HSS T32.1x100mm, 1.8μm, column temperature is 40°C, sample temperature is 15°C. For the mobile phase, solvent A was 10% acetonitrile ("ACN") in water (containing 0.1% formic acid), and solvent B was ACN. The acquisition range was 100-1500 Daltons ("Da") and the acquisition mode was electrospray ionization ("ESI")(-). Table 3 below provides the HPLC conditions.

[0067] Table 3 - HPLC conditions for the analysis of cashew seed coat extracts

[0068] running time (min) Injection volume (μL) concentration 20.00 2.00 1mg / mL

[0069] Peak identification was based on accurate mass only. Digal...

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Abstract

A botanical extract exhibits antioxidant activity, wherein the botanical extract is at least an extract from the genus Anacardium.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Patent Application No. 62 / 725,435 filed August 31, 2018, the disclosure of which is incorporated herein by reference in its entirety. technical field [0003] The present invention relates generally to inhibitors of oxidation-induced DNA damage, and more particularly to plant inhibitors of oxidation-induced DNA damage and the use of such plant inhibitors as antioxidants. Background technique [0004] Oxygen is a highly reactive atom capable of becoming part of potentially damaging molecules called "free radicals". Free radicals, commonly referred to as reactive oxygen species ("ROS"), contain one or more unpaired electrons in their outermost orbitals. Common examples of reactive oxygen species include hydroperoxy radicals (ROO*), superoxide anions (O 2 *), reactive hydroxyl groups (OH*) and hydrogen peroxide (H 2 o 2 ) free radicals. These free radicals are produced sponta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A61K36/22A61P39/06
CPCA61P39/06A61K36/22A23L33/105A23V2002/00A23V2200/02A23V2250/2116A61K31/353A61K36/77
Inventor 贾廷德·拉纳凯莉·米切尔
Owner INNOPHOS INC
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