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Cell membrane bionic polyamide-amine dendritic macromolecular hydrogel as well as preparation method and application thereof

A technology of amine dendritic and macromolecular water, which is applied in the field of cell membrane biomimetic polyamide-amine dendritic macromolecular hydrogel and its preparation, can solve problems such as easy adsorption, adhesion and inflammatory response, and achieve simple preparation method, Function enrichment and biocompatibility improvement

Active Publication Date: 2021-04-09
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the abundant amino groups on the surface of the dendrimer itself, it is easy to adsorb some negatively charged proteins on the surface when it acts in vivo, which will cause complement activation, platelet adhesion and inflammatory response, etc.

Method used

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  • Cell membrane bionic polyamide-amine dendritic macromolecular hydrogel as well as preparation method and application thereof
  • Cell membrane bionic polyamide-amine dendritic macromolecular hydrogel as well as preparation method and application thereof
  • Cell membrane bionic polyamide-amine dendritic macromolecular hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Synthesis of Cell Membrane Biomimetic Polyamide-Amine Dendrimer Hydrogel

[0040] A method for preparing a cell membrane biomimetic polyamide-amine dendrimer hydrogel, comprising the steps of:

[0041] (1) Slowly add 4mL 2mol / L dicholine phosphite (named PDPC) isopropanol solution dropwise to 8mL 25% (v / v) glutaraldehyde isopropanol solution, add 600 μL triethylamine, Stir the reaction at 0°C for 8 hours to obtain a mixed solution; wherein, phosphite dicholine salt is prepared by mixing 50mmol choline chloride and 10mmol diphenyl phosphite in 80mL dimethyl sulfoxide / pyridine mixed solvent (dimethyl sulfoxide and pyridine According to the volume ratio of 50:1.5 mixed) at room temperature for 4 hours in the system.

[0042](2) 100mg of polyamidoamine (PAMAM-G4) was dissolved in 15mL of anhydrous isopropanol to obtain a polyamidoamine dendrimer (PAMAM) solution;

[0043] (3) Slowly add the mixed solution obtained in step (1) to the polyamide-amine dendrimer (P...

Embodiment 2

[0045] Example 2: Synthesis of Cell Membrane Biomimetic Polyamide-Amine Dendrimer Hydrogel

[0046] A method for preparing a cell membrane biomimetic polyamide-amine dendrimer hydrogel, comprising the steps of:

[0047] (1) Slowly add 2mL 2mol / L phosphite dicholine salt isopropanol solution dropwise to 8mL 50% (v / v) glutaraldehyde isopropanol solution, add 300μL triethylamine, at room temperature (25 ℃) stirred and reacted for 24 hours to obtain a mixed solution; wherein, phosphite dicholine salt was prepared by 20mmol choline chloride and 10mmol diphenyl phosphite in 60mL dimethyl sulfoxide / pyridine mixed solvent (dimethyl sulfoxide and pyridine Volume ratio 50:10 mixed) at room temperature for 4 hours in the preparation.

[0048] (2) 100mg of polyamidoamine (PAMAM-G4) was dissolved in 15mL of anhydrous isopropanol to obtain a polyamidoamine dendrimer (PAMAM) solution;

[0049] (3) Slowly add the mixed solution obtained in step (1) to the polyamide-amine dendrimer (PAMAM) s...

Embodiment 3

[0051] Example 3: Protein Adsorption Behavior of Cell Membrane Biomimetic Polyamide-Amine Dendrimer Hydrogels

[0052] In PBS (pH7.4, 0.01mol / L) solution, bovine serum albumin (purchased from Sigma) was used as a model protein to study the protein adsorption behavior of the gel.

[0053] (1) The cell membrane biomimetic polyamide-amine dendrimer hydrogel (PAMAM-PC) and the control polyamide-amine dendrimer hydrogel of the same size as in Example 1 were prepared in a 48-well plate The gel block (that is, the unmodified dendrimer (PAMAM) hydrogel, its preparation method is similar to the cell membrane biomimetic polyamide-amine dendrimer hydrogel, specifically, 100mg of polyamide-amine (PAMAM- G4) Dissolve in 15mL of anhydrous isopropanol to obtain a polyamide-amine dendrimer (PAMAM) solution; then slowly add 8mL of 25% (v / v) glutaraldehyde isopropanol solution dropwise to the polyamide-amine dendrimer (PAMAM) solution; In the amine dendritic macromolecule (PAMAM) solution, vor...

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Abstract

The invention discloses cell membrane bionic polyamidoamine dendritic macromolecular hydrogel as well as a preparation method and application thereof, and belongs to the technical field of functional polymer materials. The preparation method of the cell membrane bionic polyamidoamine dendrimer hydrogel comprises the following steps: slowly dropwise adding a phosphorous acid dicholine salt (PDPC) isopropanol solution into an isopropanol solution containing a gel reaction cross-linking agent, adding triethylamine, and carrying out a stirring reaction to obtain a mixed solution; dissolving polyamidoamine (PAMAM-G4) in anhydrous isopropanol to obtain a polyamidoamine dendrimer solution; and dropwise adding the mixed solution into the polyamidoamine dendritic macromolecule solution, uniformly stirring, curing and molding to obtain hydrogel, and washing with water to obtain the product. The preparation method of the cell membrane bionic polyamidoamine dendrimer hydrogel is simple, and the biocompatibility and the biological adhesion resistance of the hydrogel can be remarkably improved.

Description

technical field [0001] The invention relates to the technical field of functional polymer materials, in particular to a cell membrane biomimetic polyamide-amine dendritic macromolecule hydrogel and its preparation method and application. Background technique [0002] Dendritic macromolecules are highly branched monodisperse macromolecules, whose molecular structure consists of a central core, repeating units, and terminal groups. Application prospect. Among them, polyamide-amine (PAMAM) dendrimers, which use ethylenediamine as the core and react with methyl acrylate through Michael addition and amidation reactions, are the most widely studied and applied dendrimers. Accurate molecular structure, high geometric symmetry, cavities in the molecule, a large number of surface functional groups, controllable molecular weight and monodisperse molecular weight distribution, nanometer size of the molecule itself, spherical shape of high algebraic molecules, etc. The advantage is th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L79/02A61K9/06A61K47/34
CPCC08J3/075A61K9/06A61K47/34C08J2379/02
Inventor 曾戎许杰盛
Owner JINAN UNIVERSITY
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