Micron-sized homogeneous biomagnetic microsphere and preparation method thereof

A magnetic microsphere and micron-scale technology, which is applied in the combined field of nanomaterials and biotechnology engineering, can solve the problems of high difficulty in process control, long preparation process, and complicated particle size control, and achieve good industrial reproducibility and responsiveness. Highly controllable conditions and high separation efficiency

Pending Publication Date: 2021-04-09
BEAVERNANO TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing preparation methods of large-diameter magnetic microspheres, the uniform particle size control is mor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Micron-sized homogeneous biomagnetic microsphere and preparation method thereof
  • Micron-sized homogeneous biomagnetic microsphere and preparation method thereof
  • Micron-sized homogeneous biomagnetic microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example

[0027] Specific example 1

[0028] Add 5g of styrene, 0.28g of azobisisobutyronitrile, 0.84g of polyvinylpyrrolidone K-300, and 100g of ethanol to a 250mL three-necked flask. The three ports are respectively connected to a nitrogen inflow pipe, a PTFE stirring paddle, and a spherical condenser. The device was stirred for 30 min under nitrogen protection at a stirring rate of 300 rpm. Subsequently, the temperature was raised to 75 degrees under nitrogen protection to initiate the polymerization. After the polymerization was carried out for 1 h, 5 g of styrene and 0.5 g of divinylbenzene were slowly introduced at intervals of 2 h, and repeated 4 times in total, so that the total amount of styrene in the reaction solution reached 25 g. The total amount of divinylbenzene reached 2 g. After the monomer was introduced, the reaction was continued until the entire polymerization process reached the end of 24 hours, and the polymerization was always protected by nitrogen. The reactio...

Example

[0031] Specific example 2

[0032] Take 1 g of sulfonated ion-exchange resin microspheres with a 5 μm uniform particle surface in specific example 1, disperse it in 150 mL of ethylene glycol, add 5 g of ferric trichloride hexahydrate, 13 g of anhydrous sodium acetate, and 5 g of polyacrylic acid (molecular weight ~ 20000). Stir vigorously at 60 degrees for 24h. The reaction suspension was transferred to a 200 mL autoclave, heated to 200 degrees and reacted for 12 h. After cooling, the product is separated by magnetic suction, and washed with pure water for several times to obtain a total amount of ~0.8 g of uniform magnetic microspheres.

Example

[0033] Specific example 3

[0034] Take 1g of carboxylated ion-exchange resin microspheres with a 5μm uniform particle surface in specific example 1, disperse it in 120mL of triethylene glycol, add 4.5g of ferric chloride, 21g of sodium citrate, and 5g of sodium polyacrylate (molecular weight ~ 200000) After vigorous stirring at 60 degrees for 24h. The reaction suspension was transferred to a 200mL autoclave, and the temperature was raised to 190°C for 24h. After cooling, the product is separated by magnetic suction, and washed with pure water for several times to obtain a total amount of ~0.8 g of uniform magnetic microspheres.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of micron-sized homogeneous magnetic microspheres. The method comprises the following steps: uniformly mixing homogeneous ion exchange resin microspheres with ferric salt, organic alkali, polyhydric alcohol and a surfactant, sufficiently adsorbing the substances on the surfaces of the ion exchange resin microspheres, transferring the ion exchange resin microspheres into a high-pressure reaction kettle, and making reacting at 170-220 DEG C for a period of time to obtain the homogeneous magnetic microspheres. According to the preparation method, the Fe3O4 nanocluster with high saturation magnetization intensity is assembled by adopting the homogeneous polymer core, so that the process is highly controllable, the particle size is uniform, the magnetic response is fast, and no residual magnetism exists. The magnetic microspheres prepared by the method can be used in the biomedical engineering fields of cell sorting, single cell sequencing and the like after further surface chemical modification.

Description

technical field [0001] The invention relates to the combined field of nanomaterials and biotechnology engineering, in particular to a method for preparing micron-sized homogeneous magnetic microspheres that can be used in scenarios such as cell sorting and single-cell sequencing. Background technique [0002] Magnetic microspheres, also known as magnetic beads, refer to submicron or micron-sized microspheres with magnetic field response behavior. At present, magnetic microspheres are mainly composed of superparamagnetic inorganic nanoparticles and non-magnetic materials. Superparamagnetic nanoparticles generally refer to ferrite-type nanocrystals, and non-magnetic materials generally refer to polymer polymerization. Compounds or silanes, complex magnetic microspheres can be gradually assembled from many interlayers. Magnetic microspheres can be widely used in various fields of biomedical engineering, such as nucleic acid extraction, protein purification, immunoassay, cell s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): H01F1/00H01F41/02
CPCH01F1/0018H01F41/02
Inventor 任辉汤俊
Owner BEAVERNANO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap