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Micron-sized homogeneous biomagnetic microsphere and preparation method thereof

A magnetic microsphere and micron-scale technology, which is applied in the combined field of nanomaterials and biotechnology engineering, can solve the problems of high difficulty in process control, long preparation process, and complicated particle size control, and achieve good industrial reproducibility and responsiveness. Highly controllable conditions and high separation efficiency

Pending Publication Date: 2021-04-09
BEAVERNANO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing preparation methods of large-diameter magnetic microspheres, the uniform particle size control is more complicated, the preparation process takes a long time, the environment is polluted, and the process control is difficult.

Method used

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  • Micron-sized homogeneous biomagnetic microsphere and preparation method thereof
  • Micron-sized homogeneous biomagnetic microsphere and preparation method thereof
  • Micron-sized homogeneous biomagnetic microsphere and preparation method thereof

Examples

Experimental program
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Effect test

specific example 1

[0028] Add 5g of styrene, 0.28g of azobisisobutyronitrile, 0.84g of polyvinylpyrrolidone K-300.84g, and 100g of ethanol into a 250mL three-necked flask. The three ports are respectively connected to the nitrogen inflow pipe, polytetrafluoroethylene stirring paddle, and spherical condenser. device, so that the device was fed with nitrogen protection and stirred for 30 min at a stirring rate of 300 rpm. Then under the protection of nitrogen, the temperature was raised to 75 degrees to initiate polymerization. After the polymerization was carried out for 1 hour, 5 g of styrene and 0.5 g of divinylbenzene were slowly introduced every 2 hours, and a total of 4 repetitions were carried out, so that the total amount of styrene in the reaction liquid reached 25 g. The total amount of divinylbenzene reaches 2 g. After the introduction of monomers, the reaction was continued until the entire polymerization process reached 24h, and the polymerization was always protected by nitrogen. Af...

specific example 2

[0032] Take 1 g of sulfonated ion-exchange resin microspheres with a surface of 5 μm in specific example 1, disperse them in 150 mL of ethylene glycol, add 5 g of ferric chloride hexahydrate, 13 g of anhydrous sodium acetate, and 5 g of polyacrylic acid (molecular weight ~ 20000) Stir vigorously at 60°C for 24h. The reaction suspension was transferred to a 200mL autoclave, and the temperature was raised to 200°C for 12 hours. After the product was cooled, it was magnetically separated, washed several times with pure water, and the total amount of homogeneous magnetic microspheres was ~0.8g.

specific example 3

[0034] Take 1g of 5 μm average particle surface carboxylated ion exchange resin microspheres in Example 1, disperse them in 120mL of triethylene glycol, add 4.5g of ferric chloride, 21g of sodium citrate, and 5g of sodium polyacrylate (molecular weight ~ 200000) Stir vigorously at 60°C for 24h. The reaction suspension was transferred to a 200mL autoclave, and the temperature was raised to 190°C for 24h. After the product was cooled, it was magnetically separated, washed several times with pure water, and the total amount of homogeneous magnetic microspheres was ~0.8g.

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Abstract

The invention discloses a preparation method of micron-sized homogeneous magnetic microspheres. The method comprises the following steps: uniformly mixing homogeneous ion exchange resin microspheres with ferric salt, organic alkali, polyhydric alcohol and a surfactant, sufficiently adsorbing the substances on the surfaces of the ion exchange resin microspheres, transferring the ion exchange resin microspheres into a high-pressure reaction kettle, and making reacting at 170-220 DEG C for a period of time to obtain the homogeneous magnetic microspheres. According to the preparation method, the Fe3O4 nanocluster with high saturation magnetization intensity is assembled by adopting the homogeneous polymer core, so that the process is highly controllable, the particle size is uniform, the magnetic response is fast, and no residual magnetism exists. The magnetic microspheres prepared by the method can be used in the biomedical engineering fields of cell sorting, single cell sequencing and the like after further surface chemical modification.

Description

technical field [0001] The invention relates to the combined field of nanomaterials and biotechnology engineering, in particular to a method for preparing micron-sized homogeneous magnetic microspheres that can be used in scenarios such as cell sorting and single-cell sequencing. Background technique [0002] Magnetic microspheres, also known as magnetic beads, refer to submicron or micron-sized microspheres with magnetic field response behavior. At present, magnetic microspheres are mainly composed of superparamagnetic inorganic nanoparticles and non-magnetic materials. Superparamagnetic nanoparticles generally refer to ferrite-type nanocrystals, and non-magnetic materials generally refer to polymer polymerization. Compounds or silanes, complex magnetic microspheres can be gradually assembled from many interlayers. Magnetic microspheres can be widely used in various fields of biomedical engineering, such as nucleic acid extraction, protein purification, immunoassay, cell s...

Claims

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Application Information

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IPC IPC(8): H01F1/00H01F41/02
CPCH01F1/0018H01F41/02
Inventor 任辉汤俊
Owner BEAVERNANO TECH
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