Stem cell exosome preparation and preparation method thereof

Abstract

The invention discloses a stem cell exosome preparation. The stem cell exosome preparation comprises mesenchymal stem cell exosome, umbilical vein endothelial cells, human serum albumin and normal saline, wherein the mesenchymal stem cell exosome is exosome extracted from human umbilical cord mesenchymal stem cells. The invention also discloses a preparation method of the stem cell exosome preparation. The method comprises the following steps: S1, culturing human umbilical cord mesenchymal stem cells; S2, preparing the mesenchymal stem cell exosome; and S3, preparing the stem cell exosome preparation. The stem cell exosome preparation is prepared by adding the mesenchymal stem cell exosome and the umbilical vein endothelial cells into a human serum albumin solution, the obtained exosome preparation can remarkably improve the proliferation and migration capacity of the umbilical vein endothelial cells and promote generation of blood vessels, and compared with cell therapy, the exosome is more stable in property. and more convenient to store and transport; and the exosome is extracted by adopting a traditional differential ultracentrifugation method, so that the purity is relatively high, and the cost is relatively low.

Description

technical field [0001] The invention belongs to the medical field, and more specifically relates to a stem cell exosome preparation. At the same time, the present invention also relates to a preparation method of stem cell exosome preparation. Background technique [0002] Stem cells are different from other cells in that they have strong self-replication function and multi-directional differentiation potential. Studies have shown that mesenchymal stem cells can treat myocardial infarction. Randomized clinical trials have proved that mesenchymal stem cells implanted in the damaged area of ​​myocardial infarction can remodel blood vessels, inhibit myocardial fibrosis, prevent ventricular remodeling, and repair cardiac rhythm cells. However, there are many problems that cannot be ignored in the application of stem cells, such as biosafety, long-term storage and transportation, and differentiation for non-therapeutic purposes. Based on the above problems that need to be solve...

AI Technical Summary

Problems solved by technology

The enzymes and chemicals carried by exosomes can not be degraded for a long time, and have no potential toxicity; after resuspended in PBS, they can be stored at -80°C for 6 months, and the biochemical activity is not affected; it is safe to treat diseases Exosomes have made important contributions to the early prevention, diagnosis and prognosis of diseases, but there is no better extraction method for the time being

At present, the methods that can be used to extract exosomes include centrifugation, filter centrifugation, density gradient centrifugation, immunomagnetic bead method, chromatography, kit method and the recently proposed rotary ultrafiltration method, etc., in which centrifugation is through the centrifugal force of substances Different samples are separated, but microvesicles and exosomes cannot be separated better. Filtration centrifugation is obtained by filtering molecules with different relative molecular masses. Although it is simple and easy to operate, the obtained samples are not pure. Density gradient centrifugation Although the obtained samples are relatively pure, it takes a long time and the operation is complicated, which is not conducive to large-scale production. The immunomagnetic bead method utilizes the specificity of antibodies on the surface of exosomes, but its effect on biological activity is unknown, and it cannot meet the needs of further experiments. Although chromatography has many advantages, it has high requirements for equipment and cannot be popularized yet.

Although there are many methods, there is no uniform standard