Preparation method and application of natural radish blue pigment
A radish blue, natural technology, applied in the field of preparation of natural radish blue pigment, can solve the problems of easy degradation, complicated substances, low content of blue pigment, etc., and achieve the effect of increasing added value and good scientific research value
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Embodiment 1
[0042] Embodiment 1, the preparation of radish blue pigment
[0043] (1) Preparation of blue pigment reactant
[0044] Prepare 0.5mg / mL 4-oxindole-3-methylglucosinolate solution, 0.29mol / L hydrogen peroxide solution, 30unit / mL, 300unit / mL tyrosinase (PPO), 30unit / mL , 300unit / mL peroxidase solution (POD); according to figure 1 Add the solution sequentially, respectively 1: substrate: 1mL 0.5mg / mL 4-oxindole-3-methylglucosinolate solution; 2: substrate + H 2 o 2 : 1mL0.5mg / mL 4-oxindole-3-methylglucosinolate solution and 14uL0.29mol / L hydrogen peroxide solution; 3: Substrate + POD: 1mL0.5mg / mL 4-hydroxy Indole-3-methylglucosinolate solution and 100uL300unit / mL peroxidase solution; 4: substrate+H 2 o 2 +POD: 1mL0.5mg / mL 4-oxindole-3-methylglucosinolate solution, 14uL0.29mol / L hydrogen peroxide solution and 100uL300unit / mL peroxidase solution; 5: Substrate+ h 2 o 2 +High POD: 1mL0.5mg / mL 4-oxindole-3-methylglucosinolate solution, 14uL0.29mol / L hydrogen peroxide solution a...
Embodiment 2
[0056] Embodiment 2, the antioxidant activity of radish blue pigment
[0057] (1) Determination of the oxygen radical absorption capacity (Oxygen radical absorbance capacity, ORAC) of purified blue pigment
[0058] Solution preparation:
[0059] 1) Phosphate buffer solution (PBS): Accurately measure an appropriate amount of phosphoric acid and dilute it with high-purity water to obtain a 75mmol / L phosphoric acid solution; weigh 8.56g of dipotassium hydrogen phosphate and dissolve it in 500mL of high-purity water, adjust the pH to 7.4 with the phosphoric acid solution to obtain 75mmol / L Phosphate buffer at pH 7.4;
[0060] 2) Sodium fluorescein (FL): 22.5mg fluorescein sodium salt was dissolved in 50ml PBS; then 50μL was dissolved in 10mL PBS;
[0061] 3) 2,2'-amidinopropane hydrochloride (AAPH): 162mg dissolved in 5mL PBS;
[0062] 4) Preparation of blue pigment: take 1 mg of blue pigment after freeze-drying and prepare 5, 10, 20, 40, 80 mg / mL blue pigment solutions with wa...
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