Preparation method of low-pollution sparassis crispa vitamin D2 extract

Abstract

The invention discloses a preparation method of a low-pollution sparassis crispa vitamin D2 extract, which comprises the following steps: S1, ultrasonically dispersing ground and sieved sparassis crispa sporocarp in an alkaline alcohol solution, simultaneously applying a pulsed electric field and ultraviolet irradiation treatment to the alkaline alcohol solution, filtering, and taking the filtrate to obtain a first extracting solution; S2, heating the first extracting solution, performing saponification reaction for 2-4 hours, performing centrifugal separation, and taking supernate to obtain a second extracting solution; S3, applying a pulsed electric field to the second extracting solution by taking a stainless steel electrode plate as a cathode and a graphite electrode plate as an anode, carrying out electrolytic reduction reaction to obtain a mixed solution, and carrying out centrifugal separation on the mixed solution to obtain a filtrate, thereby obtaining a third extracting solution; S4, carrying out rotary evaporation on the third extracting solution at 75-95 DEG C for 30-60 minutes to obtain a mixed solution, and carrying out centrifugal separation on the mixed solution to obtain a lower-layer oily substance, thereby obtaining a vitamin D2 extract. The vitamin D2 extract does not contain heavy metals, and is low in pollution, safe and reliable.

Description

technical field [0001] The invention relates to the technical field of hydrangea deep processing, in particular to a low-pollution hydrangea vitamin D 2 Method of preparation of the extract. Background technique [0002] Vitamin D is an essential fat-soluble steroid derivative for the human body. In addition to the nutritional function of regulating calcium and phosphorus metabolism, it also has the functions of anti-tumor, preventing cardiovascular disease, autoimmune disease and diabetes. Forms closely related to health are vitamin D 2 and vitamin D 3 . Vitamin D 3 It can be obtained by the isomerization of 7-dehydrocholesterol in human epidermal cells after sunlight irradiation, while vitamin D 2 The human body cannot synthesize it by itself. The incidence of children with osteoporosis, rickets and senile osteoporosis is relatively high in China. Therefore, it is necessary to seek a variety of dietary ways to supplement the body's vitamin D 2 s level. [0003] Vit...

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Problems solved by technology

[0006] In the prior art, the Chinese patent with the publication number CN201810244359.1 discloses a high-purity edible fungus vitamin D 2 The preparation process of the extract, a high-purity edible fungus vitamin D 2 The preparation process of the extract obtains vitamin D 2 , but it ignores that during the growth of fungi such as Hydrangea, there is heavy metal pollution in the growth environment, and as a large fungus, Hydrangea is very easy to enrich heavy metals, especially chromium, so the prepared vitamin D 2 Contaminants contained in the extract are higher

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