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Method for extracting high-purity fucosan sulfate from brown algae

A fucoidan sulfate, high-purity technology, applied in the field of preparation of fucoidan sulfate, can solve the problems of unsatisfactory purity, low purity and sulfate content of fucoidan sulfate

Active Publication Date: 2021-04-20
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Alboofetileh et al. (International journal of biological macromolecules, 2019, 124:131-137) compared various assisted extraction methods and found that a single cellulase assisted extraction of brown algae Nizamuddinia zanardinii The purity of fucoidan sulfate is not ideal (62.04% total sugar content, 20.06% sulfate group content)
Fu Yanhong et al. (Food Industry, 2019, 40(08):49-53) reported the compound enzyme-assisted extraction process of kelp fucoidan sulfate. The compound enzyme composition is: 1.0% cellulase, 0.5% neutral protease %, compound protease 0.5%, and pectinase 1.0%. This article found that the compound enzyme method has greatly improved the extraction yield and product purity compared with the traditional water extraction method and ultrasonic method, but the purity of the product obtained by this method is lower than that of sulfuric acid The root content is still low, the purity of the extracted product is 43.7%, and the sulfate content is 11.05%.

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  • Method for extracting high-purity fucosan sulfate from brown algae
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  • Method for extracting high-purity fucosan sulfate from brown algae

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Experimental program
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Effect test

Embodiment 1

[0023] A kind of method of extracting high-purity fucoidan sulfate ester from brown algae of the present invention, carries out according to following steps successively:

[0024] (1) Wash the fresh kelp to remove impurities such as silt, salt and mannitol on the surface, dry it in a 50°C oven, grind it into powder and pass it through a 60-mesh sieve to obtain kelp powder;

[0025] (2) Add citric acid-sodium citrate buffer solution with a pH of 4.8 into 50 g of kelp powder, and stir while adding to obtain solution I. The mass ratio of the kelp powder to the citric acid-sodium citrate buffer solution is 1 g: 17ml;

[0026] (3) Heat solution I to 50°C, add complex enzyme for enzymolysis for 1.5 hours to obtain solution II, the amount of compound enzyme added is filter paper enzyme activity 34.15 FPU, endocellulase activity 49.2 CMCU and cellobiase live 467.05 CBU;

[0027] The purchased cellulase and β-glucosidase can be mixed according to the volume ratio of 1:4~6 to make a c...

Embodiment 2

[0042] A kind of method of extracting high-purity fucoidan sulfate ester from brown algae of the present invention, carries out according to following steps successively:

[0043] (1) Wash the fresh kelp to remove impurities such as silt, salt and mannitol on the surface, dry it in a 50°C oven, grind it into powder and pass it through a 60-mesh sieve to obtain kelp powder;

[0044] (2) Add a citric acid-sodium citrate buffer solution with a pH of 4.8 into 40 g of kelp powder, and stir while adding to obtain solution I. The mass ratio of the kelp powder to the citric acid-sodium citrate buffer solution is 1 g: 15ml;

[0045] (3) Warm up solution I to 50°C, add compound enzyme for enzymolysis for 1.5 hours to obtain solution II, the amount of compound enzyme added is filter paper enzyme activity 27.23 FPU, endocellulase activity 39.36 CMCU and cellobiase live 373.64 CBU;

[0046] The source of compound enzyme is with embodiment 1;

[0047] (4) Warm up solution II to 100°C, co...

Embodiment 3

[0056] A kind of method of extracting high-purity fucoidan sulfate ester from brown algae of the present invention, carries out according to following steps successively:

[0057] (1) Wash the fresh kelp to remove impurities such as silt, salt and mannitol on the surface, dry it in a 50°C oven, grind it into powder and pass it through a 60-mesh sieve to obtain kelp powder;

[0058] (2) Add citric acid-sodium citrate buffer solution with a pH of 4.8 into 100 g of kelp powder, and stir while adding to obtain solution I. The mass ratio of the kelp powder to the citric acid-sodium citrate buffer solution is 1 g: 17ml;

[0059] (3) Heat solution I to 50°C, add compound enzyme for enzymolysis for 1.5 hours to obtain solution II, the amount of compound enzyme added is filter paper enzyme activity 68.3 FPU, endocellulase activity 98.4 CMCU and cellobiase live 934.1 CBU;

[0060] The source of compound enzyme is with embodiment 1;

[0061] (4) Warm up solution II to 95°C and perform...

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Abstract

The invention discloses a method for extracting high-purity fucoidan from brown algae, which is a method for extracting fucoidan from economic brown algae serving as a raw material by combining complex enzyme assistance and hot water extraction. By limiting the addition amount of the compound enzyme, namely adding 61.5-75.06 FPU of filter paper enzyme activity, 81-115.64 CMCU of endo-cellulase and 750.8-1117.34 CBU of cellobiase into every 100g of brown algae powder and limiting specific parameters of an extraction process, the protection of a brown algae cell wall structure on fucoidan can be effectively broken through, and under the condition that protease and pectinase are not added and auxiliary means such as microwaves and ultrasonic waves are not used, the purity of the extracted fucosan sulfate can reach 87.13%, and the content of sulfate groups is 20.25%.

Description

technical field [0001] The invention relates to a preparation method of fucoidan sulfate, in particular to a method for extracting high-purity fucoidan sulfate from brown algae. Background technique [0002] Fucoidan sulfate has become the focus of global research and development because of its multiple biological activities. It is distributed in the cell wall matrix of brown algae and is a water-soluble macromolecular polysaccharide with relatively complex components. At present, the extraction methods of fucoidan sulfate are generally based on its soluble in hot water and insoluble in ethanol, mainly including hot water extraction, acid-base extraction, microwave / ultrasonic assisted extraction, enzyme assisted extraction, etc. [0003] The invention patent with the Chinese patent number CN201410213096.X discloses a method for extracting fucoidan sulfate ester. The sodium hydroxide solution with pH 12 is used to treat the dried kelp under stirring conditions for 30 minutes,...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 武龙唐世英周慧汪秋宽任丹丹何云海杨贺琪刘思歧董秀瑜张曦文
Owner DALIAN OCEAN UNIV
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