Three-layer embedding method for improving stability of lactic acid bacteria

A technology of three-layer embedding and lactic acid bacteria, which is applied to the direction of fixing on/in organic carriers, bacteria, preserved microorganisms, etc., can solve problems such as poor stability, and achieve high fluidity, mild reaction conditions, and strong acid resistance. Effect

Active Publication Date: 2021-05-14
河北一然生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The invention proposes a three-layer embedding method for improving the stabili

Method used

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  • Three-layer embedding method for improving stability of lactic acid bacteria
  • Three-layer embedding method for improving stability of lactic acid bacteria
  • Three-layer embedding method for improving stability of lactic acid bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] S1, carry out high-density culture to bacterial strain in fermentation tank according to existing technology, make the number of live bacteria of Lactobacillus plantarum in fermented liquid reach 8.5×10 9 cfu / ml, use a butterfly centrifuge to centrifuge the fermentation liquid under the condition that the bacteria are not damaged, and concentrate the fermentation liquid by 70 times to obtain the bacteria sludge;

[0032] S2. Add embedding protection agent 1 to the bacteria slime according to the mass ratio of 1:3, wherein the embedding protection agent 1 is 5% skimmed milk powder, 95% water (mass ratio), and use a homogenizer to mix the above bacteria slime and The emulsion after the protective agent is mixed is fully mixed;

[0033] S3. Add the embedding protectant 2 according to the mass ratio of bacteria slime: protectant 2 of 1:3, wherein the embedding protectant 2 is 2% trehalose, 0.5% mannitol, 0.5% water-soluble vitamin E, 0.5% Sodium glutamate, 1% β-cyclodextri...

Embodiment 2

[0037] S1, carry out high-density culture to bacterial strain in fermentation tank according to existing technology, make the number of live bacteria of Lactobacillus plantarum in fermented liquid reach 8.5×10 9 cfu / ml, use a butterfly centrifuge to centrifuge the fermentation liquid under the condition that the bacteria are not damaged, and concentrate the fermentation liquid by 70 times to obtain the bacteria sludge;

[0038] S2. Add embedding protectant 1 to the bacteria slime according to the mass ratio of 1:2, wherein the embedding protector 1 is 30% skimmed milk powder and 70% water (mass ratio), and use a homogenizer to mix the above bacteria slime and The emulsion after the protective agent is mixed is fully mixed;

[0039] S3. Add embedding protectant 2 according to the mass ratio of bacteria slime: protectant 2 of 1:2, wherein embedding protectant 2 is 10% trehalose, 3% mannitol, 3% water-soluble vitamin E, 3% Sodium glutamate, 6% β-cyclodextrin, 6% resistant dextri...

Embodiment 3

[0043] S1, carry out high-density culture to bacterial strain in fermentation tank according to existing technology, make the number of live bacteria of Lactobacillus plantarum in fermented liquid reach 8.5×10 9 cfu / ml, use a butterfly centrifuge to centrifuge the fermentation liquid under the condition that the bacteria are not damaged, and concentrate the fermentation liquid by 70 times to obtain the bacteria sludge;

[0044] S2. Add embedding protectant 1 to the bacteria slime according to the mass ratio of 1:1, wherein the embedding protector 1 is 50% skimmed milk powder and 50% water (mass ratio), and use a homogenizer to mix the above bacteria slime and The emulsion after the protective agent is mixed is fully mixed;

[0045] S3. Add the embedding protectant 2 according to the mass ratio of bacteria slime: protectant 2 of 1:1, wherein the embedding protectant 2 is 20% trehalose, 5% mannitol, 5% water-soluble vitamin E, 5% Sodium glutamate, 10% β-cyclodextrin, 10% resist...

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Abstract

The invention relates to the technical field of microbial preparations, and provides a three-layer embedding method for improving the stability of lactic acid bacteria, which comprises the following steps: S1, carrying out high-density culture on lactic acid bacteria in a fermentation tank; S2, centrifugally separating bacterial sludge from the fermentation liquor, wherein the water content of the bacterial sludge is 5-50%; S3, uniformly mixing the bacterial sludge and a first-layer embedding protective agent according to a mass ratio of 1: (1-3), wherein the first-layer embedding protective agent is a first-layer embedding protective agent containing 5-50% of skimmed milk powder; S4, uniformly mixing the bacterial sludge and a second-layer embedding protective agent with the mixture in S3 according to the mass ratio of 1: (1-3); S5, mixing the bacterial sludge and a third-layer embedding protective agent with the mixture obtained in S4 according to the mass ratio of 1: (1-3), and then uniformly emulsifying to obtain an emulsion in S5; S6, performing cryogenic granulation on the emulsion in the step S5 by using a liquid nitrogen dripping freezing technology, so that thalli instantly enter a dormant state to obtain cryogenic granules in the step S6; and S7, drying the cryogenic granules obtained in the step S6 until the water content is 3-8%. Through the technical scheme, the problem of poor stability in the prior art is solved.

Description

technical field [0001] The invention relates to the field of microbial preparations, in particular to a three-layer embedding method for improving the stability of lactic acid bacteria. Background technique [0002] As early as the 1970s, researchers in developed countries such as Europe, America, Japan and South Korea began to develop new microecological agents to partially or completely replace the use of antibiotics and hope to improve human immunity. After decades of development, it has been Some achievements have been made in related fields. After the reform and opening up, with the development of my country's economy, people's understanding of the health system has continued to deepen, and they have gradually realized that lactic acid bacteria microecological preparations have great advantages in regulating the balance of intestinal flora and improving immunity. [0003] However, due to the poor stress resistance of lactic acid bacteria, even if the bacterium is place...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04C12N1/20C12N1/04
CPCC12N11/10C12N11/04C12N1/20C12N1/04
Inventor 赵林森申朋杨玲郭红敏马新颖任磊路江浩张士成刘佳霍世和
Owner 河北一然生物科技股份有限公司
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