MiRNA detection method based on a solid nanopore sensor, detection probe and kit.

A technology for nanopore sensors and detection probes, which is applied in the directions of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc. It can solve the problems of difficult detection, unfavorable high-throughput detection, and high cost of nanopores Improving the effectiveness of early diagnosis and treatment
CN112795628APending Publication Date: 2021-05-14NANJING UNIV OF POSTS & TELECOMM
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV OF POSTS & TELECOMM
Publication Date
2021-05-14

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Abstract

The invention discloses a miRNA detection method based on a solid nanopore sensor, a detection probe and a kit. The detection method provided by the invention comprises the following steps: taking gold nanoparticles as a carrier, and carrying out DNA probe monomolecular marking on different gold nanoparticles. When the probe and the target miRNA coexist, the DNA probe and the miRNA target molecule are hybridized and complemented, and the probe is induced to form a gold nanoparticle dimer. When the gold nanoparticle monomer and the gold nanoparticle dimer pass through the nanopore sensor, different current track characteristic signals are generated, so that the miRNA target molecule is identified. The method can be used for multi-element detection of multiple lung cancer marker miRNA biological target molecules at the same time. According to the invention, the problem that a solid-state nanopore sensor cannot detect miRNA small biomolecules is solved, multi-element joint detection of multiple lung cancer marker miRNA target molecules is realized, and the application of the silicon nitride solid-state nanopore in the field of single molecule detection is expanded.
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Description

technical field

[0001] The invention relates to a solid-state nanopore sensor detection method, in particular to a solid-state nanopore sensor-based miRNA detection method, a detection probe and a kit. Background technique

[0002] miRNAs are short non-protein-coding RNAs that regulate mRNA expression at the transcriptional or post-transcriptional level. Numerous studies have shown that miRNA plays an important role in cell proliferation, differentiation, apoptosis and metabolism. Abnormally expressed miRNAs have been detected in a variety of human malignant tumors, and are related to the occurrence, development, prediction, diagnosis, treatment and postoperative of tumors. Therefore, miRNAs, as potential markers for cancer diagnosis, prediction and treatment, have broad prospects for clinical application. miRNA plays multiple roles in non-small cell lung cancer, including playing the role of tumor-promoting gene or tumor suppressor gene in the process of lung cancer, regu...

Claims

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