Method for detecting salvianolic acid A related substances
A related substance, salvianolic acid technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of poor separation of salvianolic acid A and poor method specificity.
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Embodiment 1
[0021] Embodiment 1: the preparation of salvianolic acid A test sample
[0022] Take the Danshen medicinal material, crush it into particles with a diameter of about 2mm, add 9 times the amount each time, soak in water at 85°C for 3 times, and stir at a speed of 20 rpm, and extract with warm soaking for 2.5 hours each time; the extract is concentrated under reduced pressure to relative density 1.25 (60°C), add ethanol to make the alcohol content 70%, let it stand, filter, and recover the ethanol from the filtrate under reduced pressure and concentrate until there is no alcohol smell; add water to dilute to 10mg of salvianolic acid B per 1ml, and use 20% lemon in the aqueous solution Adjust the pH to 3.5 with sodium phosphate, add 0.4% PdCl3 as a catalyst, heat and convert at 132°C for 3.5 hours, adjust the pH of the conversion liquid to 2.6 with 20% acetic acid, centrifuge, and concentrate the supernatant under reduced pressure to contain salvianolic acid per 1ml A5 mg was sep...
Embodiment 2
[0023] Embodiment 2: High performance liquid chromatography detects the content of related substances of salvianolic acid A
[0024] 1. Solution preparation
[0025] Diluent solution: 30% acetonitrile (containing 0.1% phosphoric acid) in water as a diluent.
[0026] Reference substance solution: Accurately measure 10mg of salvianolic acid A working reference substance, put it in a 100ml measuring bottle, add diluent to dissolve and dilute to the mark, then accurately measure 0.7ml, put it in a 100ml measuring bottle, add diluent to dilute to the mark as a control solution.
[0027] Need testing solution: Accurately measure 10 mg of salvianolic acid A, place it in a 50 ml measuring bottle, add diluent to dissolve and dilute to a solution containing 0.2 mg of salvianolic acid A per 1 ml, shake well, and use it as the testing solution.
[0028] 2. Chromatographic conditions
[0029] High performance liquid chromatograph model: Agilent1260.
[0030] Chromatographic column: ACE...
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