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Specific feed for feeding springtails and preparation method thereof

A specific and feed technology, applied in the field of microorganisms, can solve the problems of small number of pests, the number of which cannot reach the test, and the rapid growth and reproduction of springtails cannot be satisfied.

Pending Publication Date: 2021-05-28
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The first purpose of the present invention is to provide a kind of specific feed for raising Collembola, to overcome the existing insect artificial feed formula, although Collembola can be reproduced, the number of the pests obtained is still relatively small, which is far below the test target. Quantity, simply can not meet the requirements of rapid growth and reproduction of Collembola

Method used

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  • Specific feed for feeding springtails and preparation method thereof
  • Specific feed for feeding springtails and preparation method thereof
  • Specific feed for feeding springtails and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This example illustrates the preparation of a specific feed.

[0023] Step 1: Take by weighing dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate heptahydrate (MgSO 4 ·7H 2 (2) 0.5g, ferrous sulfate is 0.01g, tryptophan 3g, soybean powder 200g, vitamin C 3g, sucrose 30g, agar is 20g;

[0024] Step 2: Mix the weighed medicines together, add deionized water to make the volume to 1000mL;

[0025] Step 3: Place the prepared feed in an autoclave, autoclave at 121°C for 30 minutes, and then take it out for use;

[0026] Step 4: Put the sterilized solid feed into a grinder and crush it to a size of 8-15mm×8-15mm×8-15mm, mix it with rice bran at a weight ratio of 1:1, and place it in a culture tank.

Embodiment 2

[0028] This example illustrates the preparation of a specific feed.

[0029] Step 1: Take by weighing dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate heptahydrate (MgSO 4 ·7H 2 (2) 0.5g, ferrous sulfate is 0.01g, lysine 3g, taro powder 200g, vitamin C1.5g, vitamin E1.5g, sucrose 30g, agar is 20g;

[0030] Step 2: Mix the weighed medicines together, add deionized water to make the volume to 1000mL;

[0031] Step 3: Place the prepared feed in an autoclave, autoclave at 121°C for 30 minutes, and then take it out for use;

[0032] Step 4: Put the sterilized solid feed into a grinder and crush it to a size of 8-15mm×8-15mm×8-15mm, mix it with rice bran at a weight ratio of 1:1, and place it in a culture tank.

Embodiment 3

[0034] This example illustrates the preparation of a specific feed.

[0035] Step 1: Take by weighing dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate heptahydrate (MgSO 4 ·7H 2 (2) 0.5g, ferrous sulfate is 0.01g, methionine 3g, soybean powder 200g, vitamin E 3g, sucrose 30g, agar is 20g;

[0036] Step 2: Mix the weighed medicines together, add deionized water to make the volume to 1000mL;

[0037] Step 3: Place the prepared feed in an autoclave, autoclave at 121°C for 30 minutes, and then take it out for use;

[0038] Step 4: Put the sterilized solid feed into a grinder and crush it to a size of 8-15mm×8-15mm×8-15mm, mix it with rice bran at a weight ratio of 1:1, and place it in a culture tank.

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Abstract

The invention relates to a specific feed for feeding springtails. The specific feed comprises the following components: 2-5g / L of essential amino acid, 200g / L of protein, 2-5g / L of vitamin, 1-4g / L of dipotassium phosphate, 0.1-2g / L of magnesium sulfate heptahydrate, 0.1-2g / L of potassium chloride, 0.01-1g / L of ferrous sulfate, 15-25g / L of agar and the balance of water. The essential amino acid is any one of lysine, tryptophan or methionine, the protein is taro powder or bean powder, and the vitamin is vitamin C and / or vitamin E. The invention also relates to a preparation method and a use method of the specific feed. According to the feed disclosed by the invention, the growth speed and biomass of the springtails are greatly increased, the springtails can breed one generation after being cultured for 7 days, the number of the springtails can reach over 500, and the requirement on the quantity of the springtails in the actual research work of biology and entomology can be fully met.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a specific feed for raising collembola and a preparation method thereof. Background technique [0002] Onychiurus fimeitayius mainly damages underground tuber crops such as lily and taro, causing the skin of the crops to rot and causing serious damage to the underground parts of the crops. It is common in the planting and storage periods. [0003] At present, there are many types of insect artificial feeds, and different insect species have different feed formulas, such as peach borer, corn borer, Spodoptera litura, beet armyworm, etc. However, although these insect artificial feed formulas can make collembola reproduce, However, the number of the pest obtained is still small, which is far from the number of experiments, and cannot meet the requirements of rapid growth and reproduction of the springtail, which makes it difficult to complete the precise screening test for...

Claims

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Application Information

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IPC IPC(8): A23K50/90A23K20/142A23K20/174A23K20/26A23K20/20A23K20/163A23K10/30A23K10/37
CPCA23K50/90A23K20/142A23K20/174A23K20/26A23K20/30A23K20/163A23K10/30A23K10/37Y02P60/87
Inventor 谢昀烨盛先俏贾华凑王汉荣方丽
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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