Magnetic nano-drug carrier as well as preparation method and application thereof

A magnetic nano-carrier technology, applied in drug combination, drug delivery, pharmaceutical formulation, etc., can solve problems such as refractory degradation, high toxicity, and difficulty in living body tracing, and achieve good biocompatibility and good stability.

Active Publication Date: 2021-06-01
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the nano-drug carriers reported in the existing literature have shortcomings such as (1) high toxicity, (2) difficult to degrade, and (3) difficult to trace in vivo, which makes the use of nano-drug carriers subject to certain limitations.

Method used

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  • Magnetic nano-drug carrier as well as preparation method and application thereof
  • Magnetic nano-drug carrier as well as preparation method and application thereof
  • Magnetic nano-drug carrier as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, the preparation of quaternary ammonium cationized amylose (CA)

[0045] Weigh 0.50g of amylose and add it to 25mL of distilled water, adjust the pH to 12-14 with 4mol / L NaOH solution, heat and stir to disperse; slowly add 5mL of aqueous solution containing 0.25g of active etherification agent GTA, at 50℃ Continue to stir and react for 12 hours; after the reaction, adjust the pH to neutral with hydrochloric acid solution, transfer all the solution to a cellulose dialysis bag with a molecular weight cut-off of 8000-14000 Da, dialyze with deionized water for 3 days, filter the dialysate and freeze After drying, quaternary ammonium cationized amylose was obtained, denoted as CA.

Embodiment 2

[0046] Embodiment 2, the aqueous solution of quaternary ammonium cationized amylose and superparamagnetic iron oxide nanoparticle composite Preparation of (CA-SPIO)

[0047] Weigh 0.20g quaternary ammonium cationized amylose in a 50mL flask, add 20mL distilled water and stir to dissolve; weigh 0.20g FeCl 3 ·6H 2 O and 0.10 g FeCl 2 4H 2 O was dissolved in 5 mL of distilled water and added to the above system, and stirred under nitrogen gas for 30 minutes. The flask was placed in an 80°C water bath, and 2.5 mL of 25% ammonia water was added with a syringe under vigorous stirring, and reacted for 1 hour. After the reaction was completed, the temperature was lowered to room temperature, and the entire solution was transferred to a cellulose dialysis bag with a molecular weight cut-off of 8000-14000 Da for dialysis for 2 days. After the dialysate was centrifuged to remove insoluble matter, the upper layer was stored at 4°C to obtain an aqueous solution of a complex of quat...

Embodiment 3

[0048] Embodiment 3, quaternary ammonium cationized amylose-tetraphenylethylene-superparamagnetic iron oxide nanoparticles (CA- Preparation of SPIO-TPE)

[0049] Weigh 4mg TPE and dissolve in 4mL CH 2 Cl 2 Then add 20mg aqueous solution (CA-SPIO) of quaternary ammonium cationized amylose and superparamagnetic nanoparticle complex (CA-SPIO), mix well at room temperature, then drop into 40mL pure water while ultrasonic, after ultrasonic for 1 hour, the product is transferred into into a dialysis bag with a molecular weight cut-off of 2000Da, dialyze for 3 days, change pure water twice a day, freeze-dry the dialysate, collect the solid product and store it at 4°C for later use to obtain quaternary ammonium cationized amylose-tetraphenylethylene - Superparamagnetic iron oxide nanoparticles, denoted as CA-SPIO-TPE.

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Abstract

The invention relates to the technical field of drug carriers, in particular to a magnetic nano-drug carrier as well as a preparation method and an application thereof. The magnetic nano-drug carrier comprises an SP94 targeting peptide and a coupling product of an siRNA delivery carrier, and the coupling product of the siRNA delivery carrier comprises superparamagnetic iron oxide nanoparticles and quaternary ammonium cationized amylose-tetraphenylethylene-superparamagnetic iron oxide nanoparticles formed by coupling quaternary ammonium cationized amylose and tetraphenylethylene, and the siRNA is adsorbed in the magnetic nano-drug carrier. The cytotoxicity experiments prove that the nano-drug carrier has good biocompatibility, good stability, targeting property and drug release property, and the magnetic nano-drug carrier can be applied to preparation of cancer gene therapy drugs.

Description

technical field [0001] The invention relates to the technical field of drug carriers, in particular to a magnetic nano drug carrier and its preparation method and application. Background technique [0002] In recent years, magnetic nanoparticles have been widely used in the fields of biotherapy and diagnosis due to their unique properties. Their surface chemical activity can easily bind biomacromolecules, and make them It becomes a good targeting carrier. Under the action of alternating magnetic field, magnetic particles can absorb electromagnetic wave energy and convert it into heat energy, and can confine the heat energy to tumor tissue. When the temperature exceeds 41°C, it can lead to apoptosis and necrosis of cells, thus Hyperthermia for tumors can be realized. [0003] In clinical treatment, the low toxicity and high efficiency of drugs have always been the focus of people's research, and the research on drug targeted delivery, that is, how to effectively treat drugs ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/12A61K49/14A61K49/18A61K31/713A61K9/51A61K47/64A61K47/61A61P35/00
CPCA61K49/126A61K49/14A61K49/1863A61K31/713A61K9/5161A61K47/64A61K47/61A61P35/00
Inventor 吴卓张汉臣邓立刘海晴麦思瑶
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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