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Method for in-vivo detection of antioxidant capacity of functional food or medicine and application

A technology of anti-oxidation ability and functional food, which is applied in the biological field, can solve the problems of oxidative stress and harmfulness to human body, and achieve the effect of rapid screening, fast screening work, and simple feeding method

Inactive Publication Date: 2021-06-01
FUZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although oxidative stress has been used clinically to treat some diseases such as cancer with some degree of clinical success, there is a tendency to describe oxidative stress as harmful to the human body

Method used

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  • Method for in-vivo detection of antioxidant capacity of functional food or medicine and application
  • Method for in-vivo detection of antioxidant capacity of functional food or medicine and application
  • Method for in-vivo detection of antioxidant capacity of functional food or medicine and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A Drosophila model in which temperature stress produces an increase in ROS

[0019] Create a Drosophila intestinal reactive oxygen species increase model through temperature stress stress: collect 30 wild-type Drosophila (Canton-S) males 2-5 days after eclosion, put them in normal food, and then culture them at 37°C Take it out after 15 hours in the box (too long time may cause the death of fruit flies).

[0020] Normal food contains 0.75% soybean flour (g / ml), 4.5% corn flour (g / ml), 1.5% yeast (g / ml), 0.5% propionic acid (v / v), 0.1% methylparaben (g / ml), 0.02% corn syrup (v / v), 1.25% sucrose (g / ml), 1.25% glucose (g / ml), 0.5% agar (g / ml).

[0021] Intestinal ROS level detection:

[0022] Dissolve 1 mg DHE (Sigma, product number 37291) in 1 mL of DMSO to prepare a 3 mmol / L stock solution, take 20 µL and dissolve it in 1 mL of PBS to obtain a DHE staining solution with a concentration of 60 µmol / L, which is now prepared use, keep away from light;

[0023] Dissolve 8...

Embodiment 2

[0028] Antioxidant effect of royal jelly

[0029] The antioxidant effect of royal jelly was analyzed. Specific plan: First, prepare food containing royal jelly, fresh royal jelly is mixed with 0.75% soybean flour (g / ml), 4.5% corn flour (g / ml), 1.5% yeast (g / ml) with a final mass fraction of 20%. ml), 0.5% propionic acid (v / v), 0.1% methylparaben (g / ml), 0.02% corn syrup (v / v), 1.25% sucrose (g / ml), 1.25% glucose (g / ml), 0.5% agar (g / ml) food. The control was normal food (that is, food without adding fresh royal jelly).

[0030] Collect 60 wild-type Drosophila (Canton-S) males 2-5 days after eclosion and divide them into two groups. After heat shock treatment in the incubator for 15 hours, take it out. Then DHE staining was carried out according to the detection method of intestinal ROS level and photographed, and the fluorescence intensity value was obtained by using ImageJ (https: / / imagej.net / ) fluorescence intensity analysis software, and the difference in fluorescence...

Embodiment 3

[0034] Antioxidant effect of melatonin

[0035]The antioxidant effect of melatonin was analyzed. Specific plan: First, prepare food containing melatonin (Shanghai Sangon Biotechnology, product number A600605). Melatonin is mixed with 0.75% soybean powder (g / ml), 4.5% corn powder (g / ml), 1.5% yeast (g / ml), 0.5% propionic acid (v / v), 0.1% methylparaben (g / ml), 0.02% corn syrup (v / v), 1.25 % sucrose (g / ml), 1.25% glucose (g / ml), 0.5% agar (g / ml) food. The control was normal food (that is, food without added melatonin).

[0036] Collect 60 wild-type Drosophila (Canton-S) males 2-5 days after eclosion, and divide them into two groups. Take out after heat shock treatment in 37°C incubator for 15 hours. Then DHE staining was carried out according to the detection method of intestinal ROS level and photographed, and the fluorescence intensity value was obtained by using ImageJ (https: / / imagej.net / ) fluorescence intensity analysis software, and the difference in fluorescence betwee...

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Abstract

The invention relates to a method for in-vivo detection of antioxidant capacity of functional food or medicine and application. According to the method, a fruit fly intestinal reactive oxygen species (ROS) rising model is manufactured through temperature and pressure stress, then the model is fed with functional food or medicine, the intestinal tract is dissected, dyeing of an ROS fluorescent probe dihydroethidium (DHE) is carried out, and the change of the ROS level is evaluated. Through the model and the evaluation means established in the invention, the method has the advantages of simple operation and strong repeatability, can be used for large-scale research and screening of the antioxidant effect of functional food or drugs, and have good application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method and application for detecting the antioxidant capacity of functional food or medicine in a living body. Background technique [0002] Oxidative stress is a phenomenon caused by an imbalance between the production and accumulation of reactive oxygen species (ROS) in cells and tissues and the ability of biological systems to detoxify these products. Oxygen ions, peroxides, and oxygen-containing free radicals are common ROS, which are usually by-products of the metabolism of biological systems and can play a variety of physiological roles, such as cell signaling. Processes such as protein phosphorylation, activation of multiple transcription factors, apoptosis, immunity and differentiation all depend on proper ROS production. The presence of ROS inside cells needs to be kept at low levels, and when ROS production increases, they can have detrimental effects on impo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/28
CPCG01N1/2813G01N21/6428G01N21/6486G01N2021/6432
Inventor 陈文锋申磊陈平华谢甲钰余凌奇杜晓月
Owner FUZHOU UNIV
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