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Multi-sinus combined cerebral venous thrombosis animal model and construction method thereof

An animal model, cerebral vein technology, applied in the field of medicine, to achieve the effect of reducing mortality, simple operation and good stability

Active Publication Date: 2021-06-04
XUANWU HOSPITAL OF CAPITAL UNIV OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Most of the above animal models induce simple superior sagittal sinus thrombosis, multiple sinus combined with thrombosis and severe CVT models with large area of ​​venous cerebral infarction. See the report

Method used

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  • Multi-sinus combined cerebral venous thrombosis animal model and construction method thereof
  • Multi-sinus combined cerebral venous thrombosis animal model and construction method thereof
  • Multi-sinus combined cerebral venous thrombosis animal model and construction method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1. Enflurane was used to induce anesthesia, and 70% nitrous oxide and 30% oxygen were sent through an anesthesia mask to maintain the entire operation and monitoring process. The anesthetized rats were fixed in a prone position on a stereotaxic frame, and the body temperature was maintained at 37°C with a constant temperature blanket throughout the operation ( figure 1 ).

[0052] 2. After skin preparation, make a surgical incision about 1.5cm in the middle of the head, separate the subcutaneous tissue, expose the skull, drill a hole with a grinding drill on the side between the anterior bregma and the herringbone point, operate under a microscope, and gradually expand In order to form a 10mm×3mm bone window, expose the sss and the cortex on both sides, and keep the dura mater intact, the drill bit was continuously cooled with normal saline during drilling to avoid burning the dura mater and cortex ( figure 2 ).

[0053] 3. After the SSS was exposed, a laser scatter...

Embodiment 2

[0062] In order to illustrate the repeatability of the animal model construction method of the present invention, table 2 has listed the cerebral blood flow change situation of 20 rats before half ligation, after half ligation and application ferric chloride thrombin, we will half ligation, before half ligation, The cerebral blood flow after semi-ligation and application of ferric chloride thrombin is expressed in the form of mean ± standard deviation, that is, 359.42±72.61PU, 189.98±31.12PU, 95.93±28.31PU, calculated by one-way analysis of variance F =151.90, P Figure 11 ), suggesting that thrombus was formed, indicating that the construction method has good repeatability.

[0063] Table 2. Changes in cerebral blood flow at different stages during modeling

[0064]

Embodiment 3

[0066] For the 20 rat cerebral venous thrombosis animal models constructed in Example 2, 24 hours after modeling, one died of epidural hematoma, and the 24-hour survival rate after operation was 95%. Two each died of severe cerebral ischemia, and the mortality rate at 1 week after operation was 25% (5 / 20), among which the mortality rate due to severe cerebral ischemia was 21.1% (4 / 19), which is similar to that of human severe cerebral ischemia. CVT mortality rates are relatively similar. Twelve hours after the operation, 5 rats were deeply anesthetized and perfused with saline heart. It was found that all 5 rats had thrombosis in the superior sagittal sinus, transverse sinus and internal jugular vein, and even cortical vein thrombosis (see Figure 12 A, The yellow arrow indicates the superior sagittal sinus thrombosis, the green clipped head indicates the transverse sinus thrombosis, the blue arrow indicates the internal jugular vein thrombosis, and the black arrow indicates t...

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Abstract

The invention relates to the technical field of medicines, and particularly discloses a multi-sinus combined cerebral venous thrombosis animal model and a construction method thereof. A severe CVT animal model with multiple venous sinus combined thrombus (such as superior sagittal sinus + transverse sinus, superior sagittal sinus + transverse sinus + internal jugular vein), large-area venous cerebral infarction, cerebral hemorrhage and other pathological changes, and with more serious neurological dysfunction and longer thrombus duration is induced through a method of combining semi-ligation with ferric chloride and thrombin; and the model can be used for research on pathological and physiological mechanisms of severe CVT as well as research and evaluation on treatment strategies, so that treatment of patients with severe CVT and promotion of good prognosis are possibly facilitated, new targets are explored, novel drugs are researched and developed, and the death rate of the patients with severe CVT is reduced.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an animal model of cerebral venous thrombosis. Background technique [0002] Cerebral venous thrombosis (CVT) is a special type of cerebrovascular disease different from arterial stroke. It mainly affects young people and children. It is an important cause of stroke in young and middle-aged people. An increasing trend, an epidemiological survey from Australia showed that the annual incidence of CVT was 1.57 / 100,000 people / year, which was higher than the previously reported 1.3 / 100,000 people, and the lesion sites were multiple venous sinuses [57.14% ], superior sagittal sinus [16.19%], transverse sinus [11.43%] and sigmoid sinus [8.57%]. Venous cerebral infarction or hemorrhage occurs in about 60% of CVT patients, that is, local edema and hemorrhage of brain tissue caused by occluded cerebral venous sinuses or cortical veins. CVT with venous cerebral infarction or hemorrhage b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 段建钢肖立坡吉训明
Owner XUANWU HOSPITAL OF CAPITAL UNIV OF MEDICAL SCI
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