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Pandan leaf freeze-dried powder and preparation process thereof

A preparation process and technology of pandan leaves, applied in the field of freeze-dried pandan leaf powder and its preparation, can solve problems such as poor product stability and destruction of active ingredients

Inactive Publication Date: 2021-06-04
海南热作高科技研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN111248426A discloses a pandan leaf product and its preparation method, which has increased the content of squalene, etc., but its active ingredients are still destroyed in a large amount, and the product stability is not good

Method used

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  • Pandan leaf freeze-dried powder and preparation process thereof
  • Pandan leaf freeze-dried powder and preparation process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 preparation of pandan leaf freeze-dried powder

[0027] S1. Pretreatment: Wash and chop 1 kg of fresh pandan leaves, put them into 2.0 kg of pretreatment liquid for soaking for 3 minutes, and then take out the pandan leaves. The pretreatment liquid contains 0.8% citric acid and 0.13% vitamin C in mass concentration ;

[0028] S2, low-temperature fermentation: add 1.5kg of 2wt% ethanol solution to the pretreated pandan leaves, then add 0.13kg of chitosan and 0.04kg of thraustochytrium powder, and place at -8°C for low-temperature fermentation for 1.5h to obtain fermentation broth;

[0029] S3. Composite enzymatic hydrolysis: Add 6 mg of cellulase, 4 mg of protease and 1.5 mg of pectinase to the fermentation broth, pretreat with ultrasonic at 230W for 2 minutes, and then enzymatically hydrolyze at 40°C for 5 minutes to obtain the enzymatic hydrolysis solution, which is filtered and centrifuged to obtain Centrate;

[0030] S4. Freeze-drying: Concentrate the...

Embodiment 2

[0031] Embodiment 2 preparation of pandan leaf freeze-dried powder

[0032] S1. Pretreatment: Wash and chop 1 kg of fresh pandan leaves, put them into 2.5kg of pretreatment liquid and soak for 2 minutes, then take out the pandan leaves. The pretreatment liquid contains 0.5% citric acid and 0.2% vitamin C in mass concentration ;

[0033] S2, low-temperature fermentation: add 1.2kg of 1wt% ethanol solution to the pretreated pandan leaves, then add 0.1kg of chitosan and 0.03kg of thraustochytrium powder, and place it at -10°C for low-temperature fermentation for 2 hours to obtain fermentation liquid;

[0034] S3. Composite enzymatic hydrolysis: Add 5 mg of cellulase, 5 mg of protease and 2 mg of pectinase to the fermentation broth, pretreat with 200W ultrasonic for 2 minutes, and then enzymatically hydrolyze at 35°C for 10 minutes to obtain the enzymatic hydrolysis solution, which is filtered and centrifuged to obtain centrifuged liquid;

[0035] S4. Freeze-drying: Concentrate...

Embodiment 3

[0036] Example 3 Preparation of Banlan Leaf Freeze-dried Powder

[0037] S1. Pretreatment: Wash and chop 1 kg of fresh pandan leaves, put them into 1.5kg of pretreatment liquid and soak for 5 minutes, and then take out the pandan leaves. The pretreatment liquid contains 1% citric acid and 0.1% vitamin C in mass concentration ;

[0038] S2, low-temperature fermentation: add 1.8kg of 3wt% ethanol solution to the pretreated pandan leaves, then add 0.2kg of chitosan and 0.05kg of thraustochytrium powder, and place it at -5°C for low-temperature fermentation for 1 hour to obtain fermentation liquid;

[0039] S3. Composite enzymatic hydrolysis: Add 8 mg of cellulase, 3 mg of protease and 1 mg of pectinase to the fermentation broth, pretreat with 300W ultrasonic for 1 min, and then enzymatically hydrolyze at 40°C for 5 min to obtain the enzymatic hydrolyzate, which is filtered and centrifuged to obtain centrifuged liquid;

[0040] S4. Freeze-drying: Concentrate the centrifugate, f...

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Abstract

The present invention provides pandan leaf freeze-dried powder and a preparation process thereof. The preparation process comprises the following steps: S1, washing and cutting up fresh pandan leaves, putting the cut-up pandan leaves into a pretreatment solution to be soaked, and then taking out the pandan leaves, wherein thepretreatment solutioncontains citric acid and vitamin C; S2, adding the pretreated pandan leaves to an ethanol solution, and then adding chitosan and thraustochytrium limacinum powder, wherein the mass ratio of the ethanol solution to the chitosan to the fresh pandan leaves to the thraustochytrium limacinum to the fresh pandan leaves is (1.2-1.8): (0.1-0.2): (0.03-0.05): 1; and carrying out low-temperature fermentation at the temperature of subzero 10 DEG C to subzero 5 DEG C to obtain fermentation liquor; S3, adding cellulase, protease and pectinase to the fermentation liquor; carrying out ultrasonic pretreatment at 200-300 W, carrying out enzymolysis at 35-40 DEG C to obtain an enzymatic hydrolysate, and carrying out filtering and centrifuging to obtain a centrifugate; and S4, concentrating the centrifugate, performing freeze-drying at-20 to-15 DEG C, and then performing freeze-drying at-30 to-25 DEG C to obtain the product. The prepared pandan leaf freeze-dried powder is high in quality and high in stability.

Description

technical field [0001] The invention relates to the technical field of tropical medicinal plants, in particular to a freeze-dried pandan leaf powder and a preparation process thereof. Background technique [0002] my country's pandan leaves are imported crops. They were first introduced to the Xinglong area of ​​Wanning City, Hainan Province, and then gradually developed to other areas of Hainan and Yunnan. Today, some provinces such as Guangdong and Taiwan are also planted with pandan leaves. Banlan leaf is a characteristic resource in Hainan. It has a wide range of uses, great production potential, a wide variety of products, high added value, rich cultural connotations, and conforms to the new concept of green, healthy, and high-quality consumption. [0003] Banlan leaves, scientific name pandanus, is a perennial evergreen horticultural crop. Banlan leaves have a unique "Zongxiang" fragrance, and Banlan leaves are rich in active ingredients such as squalene, 2-acetyl-1-p...

Claims

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Application Information

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IPC IPC(8): A61K36/88A61K9/19A61P35/00A61P37/04A61J3/02A23L33/105A23P10/00
CPCA61K36/88A61K9/19A61P35/00A61P37/04A61J3/02A23L33/105A23P10/00A61K2236/10A61K2236/19A61K2236/51A23V2002/00
Inventor 欧阳欢邓福明蔡海滨廖子荣朱家立
Owner 海南热作高科技研究院有限公司
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