Detection kit for cystatin C
A technology for detecting kits and cystatin, which is applied in the field of cystatin C detection kits, can solve problems such as poor accuracy, low detection sensitivity, and complicated operation, and achieve easy operation, good specificity, and high detection accuracy Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0044] The embodiment of the present application provides a cystatin C detection kit, comprising the following steps:
[0045] All glassware was soaked in aqua regia (HNO 3 :HCL=1:3) for several hours, then rinse with ultra-pure water.
[0046] Preparation of gold nanoparticles (AuNPs) solution:
[0047] In a 250ml flask add 0.25mM, 100ml of HAuCl 4 aqueous solution and heated to boiling with vigorous stirring. Then quickly add 9.7mM, 10ml of sodium citrate solution. The color of the solution changed from bright yellow to wine red within two minutes. The mixed solution was then boiled for 15 minutes, stirred and cooled down to room temperature to obtain a gold nanoparticle solution. Store in a refrigerator at 4°C before experimentation.
[0048] Preparation of aminated upconversion nanocrystals (UCNPs) solution:
[0049] a) NaYF 4 :18%Yb 3+ ,2%Er 3+ Upconversion nanocrystals were prepared by thermally decomposing Yb 3+ / Er 3+ Powder mixed into β-NaYF 4 . The prep...
Embodiment 2
[0059] The embodiment of the present application adopts the detection kit of embodiment 1 to establish a standard detection curve of cystatin C, including the following steps:
[0060] 1.0 mL of standard solutions with different cystatin C concentrations were mixed with 50 μl of papain solution to obtain mixture 1.
[0061] Mixture 1 was reacted with 60 μl of protamine solution at 37° C. for 30 minutes to obtain mixture 2 .
[0062] Mixture 2, 80 μl of gold nanoparticle solution, and 50 μl of aminated upconversion nanocrystal solution were reacted at 37° C. for 30 minutes to obtain mixture 3 .
[0063] A 980nm laser was used to irradiate the above-mentioned mixture 3, and the fluorescence spectrum of the mixture 3 was measured. The number of measurements for a single sample was three times, and a standard detection curve was established.
[0064] The principle of the method for detecting cystatin C based on the FRET effect in the embodiment of the present application is as fo...
Embodiment 3
[0071] The embodiment of the present application adopts the detection kit of embodiment 1 to detect cystatin C, including the following steps:
[0072] Mix 1.0 mL of standard serum sample with 50 μl of papain solution to obtain mixture 1.
[0073] Mixture 1 was reacted with 60 μl of protamine solution at 37° C. for 30 minutes to obtain mixture 2 .
[0074] Mixture 2, 80 μl of gold nanoparticle solution, and 50 μl of aminated upconversion nanocrystal solution were reacted at 37° C. for 30 minutes to obtain mixture 3 .
[0075] The above-mentioned mixture 3 was irradiated with a 980nm laser, and the fluorescence spectrum of the mixture 3 was measured, and the number of determinations for a single sample was three times.
[0076] According to the standard detection curve in Example 2, the cystatin C content in the standard serum sample was detected to be 0.67 μg / ml.
PUM
| Property | Measurement | Unit |
|---|---|---|
| electric potential / voltage | aaaaa | aaaaa |
| electric potential / voltage | aaaaa | aaaaa |
| concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More