Fingered citron dietary fiber with weight-losing and lipid-lowering functions as well as preparation method and application of fingered citron dietary fiber
A technology of dietary fiber and bergamot, which is applied to the field of bergamot dietary fiber and its preparation, can solve the problems of immaturity, poor product color and water holding capacity, difficult to control fermentation time and environment by fermentation method, etc.
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Embodiment 1
[0044] The method that embodiment 1 bergamot dietary fiber adopts composite enzymatic hydrolysis process to prepare
[0045] S1. Put the bergamot slices into a hot air drying oven at 55°C and dry to constant weight, crush the dried bergamot slices through a 60-mesh sieve, put them in a ziplock bag and place them in a drying dish for later use;
[0046] S2. The bergamot powder obtained in S1 is subjected to compound enzymatic hydrolysis to obtain bergamot dietary fiber;
[0047] Wherein, the technological condition of compound enzymolysis described in step S2 is:
[0048] S21. Pretreatment: mix bergamot powder and water evenly at a mass ratio of 1:20, and gelatinize at 80°C for 40 minutes;
[0049] S22. The first step of enzymatic hydrolysis: the gelatinized solution in S21 is cooled to 60°C, the pH is adjusted to 5.5, and 1:1 α-amylase and glucoamylase are added at an enzyme amount of 396u / g to enzymatically hydrolyze for 120 minutes to degrade part of the starch;
[0050] S2...
Embodiment 2
[0052] Example 2 Research on the compound enzymatic hydrolysis process of bergamot dietary fiber α-amylase and glucoamylase
[0053] 1. Based on the preparation method in Example 1, optimize the compound enzymolysis process of α-amylase and glucoamylase in step S22. On the basis of the single factor experiment, the enzymatic hydrolysis pH was determined to be 5.5, and the three factors that had the most significant influence on the total dietary fiber yield of bergamot were selected as the independent variables, including A enzymatic hydrolysis time, B enzymatic hydrolysis temperature, and C enzyme amount. Three levels were taken for each factor, coded by -1, 0, +1, and the yield of total dietary fiber was used as the response value (Y). According to the Box-Behnken central combination design principle, three levels were designed using Design-Expert8.0.6 software. Three-level response surface analysis of factors. The coded value and real value of each factor are shown in Tabl...
Embodiment 3
[0068] Example 3 Research on Enzymolysis Process of Alkaline Protease of Bergamot Dietary Fiber
[0069] 1. Based on the preparation method in Example 1, optimize the alkaline protease enzymatic hydrolysis process in step S23. On the basis of single factor experiments, the enzymatic hydrolysis temperature was determined to be 60°C, and the effects of alkaline protease enzymatic hydrolysis time (A), enzymatic hydrolysis pH (B) and enzyme amount (C) on the yield of bergamot total dietary fiber were selected The most significant factor, according to the Box-Behnken central combination design principle, took the bergamot dietary fiber yield (%) as the response value, and carried out the response surface optimization test. The coded value and real value of each factor are shown in Table 4.
[0070] Table 4
[0071]
[0072] 2. Proteolysis and extraction of bergamot dietary fiber response surface test and results are shown in Table 5.
[0073] table 5
[0074]
[0075] 3. C...
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