Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Slow-release algal inhibition microsphere as well as preparation method and application thereof

A microsphere and slow-release technology, applied in the field of slow-release algae-inhibiting microspheres and their preparation, can solve the problem of lack of long-term, high-efficiency inhibitory effect of microcystin MCs, inability to achieve long-term and stable algae growth inhibitory effect, and complex production process. and other problems, to achieve the effect of improving the algae inhibition effect, the embedding efficiency and the simple preparation process.

Active Publication Date: 2021-06-11
CHINA AGRI UNIV
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The algae inhibitor slow-release technology uses physical and chemical means to store effective algae-inhibiting ingredients in continuous-release microspheres (continuous-release microspheres, CRM), and then release them slowly and continuously. The preparation of existing CRM and its anti-algae The effect has the following disadvantages: the production process is complicated and the production cost is high; the effect of inhibiting algae growth cannot be achieved for a long time and stable, and the effect needs to be further strengthened; while inhibiting the growth of algae, it lacks a long-term and high-efficiency inhibitory effect on the release of microcystin MCs; There is no report on the effect of CRM on inhibiting algae and inhibiting MCs under different environmental conditions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Slow-release algal inhibition microsphere as well as preparation method and application thereof
  • Slow-release algal inhibition microsphere as well as preparation method and application thereof
  • Slow-release algal inhibition microsphere as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] This embodiment provides a slow-release algae-inhibiting microsphere, the raw materials of which include:

[0036]

[0037] Note: The above denominator v is the volume of the mixed solution of luteolin emulsified system and sodium alginate described in the preparation method of slow-release algae-inhibiting microspheres.

[0038] Chitosan 0.25% (w / v)

[0039] Calcium Chloride 4% (w / v)

[0040] Note: the above (chitosan, calcium chloride) denominator v is the volume of the aqueous solution of chitosan and calcium chloride described in the preparation method of slow-release algae-inhibiting microspheres.

[0041] Glutaraldehyde 0.5% (v / v, denominator v is the total volume after the mixed solution of luteolin and sodium alginate described in the preparation method of slow-release algae-inhibiting microspheres is dropped into the aqueous solution of chitosan and calcium chloride ).

[0042] The preparation method of the above-mentioned slow-release algae-inhibiting mi...

Embodiment 2

[0049] The luteolin release characteristic test of the slow-release algae-inhibiting microspheres provided in Example 1:

[0050] Put 0.02g / mL slow-release algae-inhibiting microspheres (containing luteolin equivalent of 0.41mg / mL) into the culture medium, and cultivate them at 25±1°C under 2000lux light (12h light / 12h dark).

[0051] The cumulative release of luteolin can be measured by replacing the same amount of fresh medium every 1.5 days. The results show that the release of luteolin increased to 171.79 μg / mL on the first day, then decreased and stabilized at a lower level Until the 49th day, there was a slight increase from the 10th to the 13th day. From the 22nd to the 49th day, the cumulative release amount was 300.27-383.58 μg / mL, accounting for 73.32%-81.36% of the total content of luteolin in the slow-release algae-inhibiting microspheres.

[0052] The actual concentration of the released luteolin can be measured by regularly sampling without changing the medium. ...

Embodiment 3

[0054] The slow-release algae-inhibiting microspheres provided in Example 1 are tested for the efficient and lasting inhibitory effect on the growth of Microcystis:

[0055] Algae species tested: Microcystis aeruginosa FACHB-915.

[0056] Method: Microcystis in the exponential growth phase was inoculated on BG11 medium, and the initial density of algae cells was 4×10 5 cells / mL, in line with the algal density of Microcystis blooms, according to the dose and release characteristics of luteolin in the slow-release algae-inhibiting microspheres, add slow-release algae-inhibiting microspheres to the Microcystis culture medium to make it The concentration reaches 0.004g / mL, and at the same time, set 0.082mg / mL pure luteolin (equal to the amount of luteolin contained in the slow-release algae-inhibiting microspheres), 0.004g / mL blank microspheres (excluding luteolin) Microcystis culture fluid and other treatment groups, and the Microcystis culture fluid without any additives were u...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides slow-release algal inhibition microspheres as well as a preparation method and application thereof. The slow-release algal inhibition microspheres are prepared from the following raw materials: 1%-1.5% (w / v) of luteolin, 18%-20% (w / v) of olive oil, 10%-12% (w / v) of an emulsifier, 10%-12% (w / v) of a co-emulsifier, 1%-2.5% (w / v) of sodium alginate, 0.2%-0.3% (w / v) of chitosan, 4%-8% (w / v) of calcium chloride and 0.5%-1% (v / v) of a cross-linking agent. The slow-release algal inhibition microspheres provided by the invention can slow down and control the release rate of luteolin, the preparation process is simple, the cost is low, no pollution is caused to the environment, growth of microcystis can be remarkably and efficiently inhibited within 30-50 days through continuous and slow release of luteolin, meanwhile, release of microcystins (MCs) is efficiently inhibited, repeated addition of an algal inhibition agent is not needed, and the long-term and stable effect on efficiently inhibiting cyanobacterial bloom outbreak is achieved.

Description

technical field [0001] The invention relates to the technical field of water body environmental protection, in particular to a slow-release algae-inhibiting microsphere and a preparation method and application thereof. Background technique [0002] In recent years, cyanobacteria blooms have occurred frequently in many large lakes and reservoirs, and Microcystis is the dominant species of blooms, which can damage aquatic ecosystems by blocking sunlight and consuming oxygen. Microcystis usually produces and releases algal toxins to further pollute water bodies and poison aquatic organisms and humans, among which microcystins (MCs) are the most representative. The World Health Organization recommends that the content of MCs in drinking water be set at 1 μg / L, so controlling the growth of harmful algae and mitigating the release of MCs has become a widespread concern. [0003] The treatment of harmful algae can be divided into physical, chemical and biological methods. However...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/28A01N43/16A01P13/00B01J13/14
CPCA01N25/28A01N43/16B01J13/14
Inventor 李洁明李碧滢安广琪
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products