Slow-release algal inhibition microsphere as well as preparation method and application thereof
A microsphere and slow-release technology, applied in the field of slow-release algae-inhibiting microspheres and their preparation, can solve the problem of lack of long-term, high-efficiency inhibitory effect of microcystin MCs, inability to achieve long-term and stable algae growth inhibitory effect, and complex production process. and other problems, to achieve the effect of improving the algae inhibition effect, the embedding efficiency and the simple preparation process.
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Embodiment 1
[0035] This embodiment provides a slow-release algae-inhibiting microsphere, the raw materials of which include:
[0036]
[0037] Note: The above denominator v is the volume of the mixed solution of luteolin emulsified system and sodium alginate described in the preparation method of slow-release algae-inhibiting microspheres.
[0038] Chitosan 0.25% (w / v)
[0039] Calcium Chloride 4% (w / v)
[0040] Note: the above (chitosan, calcium chloride) denominator v is the volume of the aqueous solution of chitosan and calcium chloride described in the preparation method of slow-release algae-inhibiting microspheres.
[0041] Glutaraldehyde 0.5% (v / v, denominator v is the total volume after the mixed solution of luteolin and sodium alginate described in the preparation method of slow-release algae-inhibiting microspheres is dropped into the aqueous solution of chitosan and calcium chloride ).
[0042] The preparation method of the above-mentioned slow-release algae-inhibiting mi...
Embodiment 2
[0049] The luteolin release characteristic test of the slow-release algae-inhibiting microspheres provided in Example 1:
[0050] Put 0.02g / mL slow-release algae-inhibiting microspheres (containing luteolin equivalent of 0.41mg / mL) into the culture medium, and cultivate them at 25±1°C under 2000lux light (12h light / 12h dark).
[0051] The cumulative release of luteolin can be measured by replacing the same amount of fresh medium every 1.5 days. The results show that the release of luteolin increased to 171.79 μg / mL on the first day, then decreased and stabilized at a lower level Until the 49th day, there was a slight increase from the 10th to the 13th day. From the 22nd to the 49th day, the cumulative release amount was 300.27-383.58 μg / mL, accounting for 73.32%-81.36% of the total content of luteolin in the slow-release algae-inhibiting microspheres.
[0052] The actual concentration of the released luteolin can be measured by regularly sampling without changing the medium. ...
Embodiment 3
[0054] The slow-release algae-inhibiting microspheres provided in Example 1 are tested for the efficient and lasting inhibitory effect on the growth of Microcystis:
[0055] Algae species tested: Microcystis aeruginosa FACHB-915.
[0056] Method: Microcystis in the exponential growth phase was inoculated on BG11 medium, and the initial density of algae cells was 4×10 5 cells / mL, in line with the algal density of Microcystis blooms, according to the dose and release characteristics of luteolin in the slow-release algae-inhibiting microspheres, add slow-release algae-inhibiting microspheres to the Microcystis culture medium to make it The concentration reaches 0.004g / mL, and at the same time, set 0.082mg / mL pure luteolin (equal to the amount of luteolin contained in the slow-release algae-inhibiting microspheres), 0.004g / mL blank microspheres (excluding luteolin) Microcystis culture fluid and other treatment groups, and the Microcystis culture fluid without any additives were u...
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