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Bio-organic fertilizer for enhancing disease resistance and stress resistance of crops and preparation method thereof

A bio-organic fertilizer and crop technology, which is applied in the field of agricultural soil bio-fertilizer, can solve the problems of crop product quality reduction, crop nutrient absorption imbalance, crop yield reduction in cultivated land, etc., achieve less pathogenic bacteria, help water retention, and promote crop growth. The effect of growth

Inactive Publication Date: 2021-06-11
广西沃瑞德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Due to the unreasonable use of chemical fertilizers and chemical pesticides in agricultural production, soil compaction of cultivated land, low organic matter content, unbalanced crop nutrient absorption, reduced quality of crop products, reduced yield of cultivated land crops, enhanced resistance to diseases and insect pests, and excessive pesticide residues in agricultural products have caused a series of problems. These problems have brought challenges to the sustainable development of agriculture and ecological environmental protection. How to solve these problems has become a new topic for agricultural technicians

Method used

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  • Bio-organic fertilizer for enhancing disease resistance and stress resistance of crops and preparation method thereof
  • Bio-organic fertilizer for enhancing disease resistance and stress resistance of crops and preparation method thereof
  • Bio-organic fertilizer for enhancing disease resistance and stress resistance of crops and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of complex bacterial solution

[0026] Lactobacillus kitasato and Sinorhizobium melilotus were collected respectively. After activation, the activated colonies were picked respectively, inserted into 1L LB culture medium, placed in a constant temperature shaking incubator at 26°C, and cultured at 180rpm for 36 hours to form a bacterial solution.

[0027] Place the obtained bacterial liquid in the fermenter and cultivate it in the PDA culture medium, so that the target bacterial strain in the fermenter reaches 1×10 8 ~1×10 9 cfu / ml.

[0028] The two cultured strains and the purchased yeast are mixed according to the colony forming unit ratio of 1:(5-6):(6-7) to obtain a composite bacterial liquid.

Embodiment 2

[0030] 1. Preparation of bio-organic fertilizer 2

[0031] S1. Mix 55 parts of poultry manure, 30 parts of dregs, 30 parts of straw and 40 parts of bagasse, and then pulverize;

[0032] Livestock manure is a mixture of pig manure and cow manure, with a mass ratio of 1:2;

[0033] The medicinal dregs include 30 parts of ephedra, 25 parts of rhubarb, 30 parts of isatidis and 30 parts of rhubarb in terms of mass parts;

[0034] Straws include corn stalks and sesame stalks with a mass ratio of 2:3.

[0035] S2. Adjust the water content of the mixture pulverized material to 35%;

[0036] S3. Inoculate the composite bacterial liquid, stack retting into a conical shape for fermentation, turn the fermented material when the temperature rises above 45°C during the stacking fermentation process, and ferment for 10 days to obtain decomposed clinker;

[0037] The inoculum amount of the compound bacteria solution is 1×10 5 ~1×10 6 cfu / g;

[0038] S4. Pass the decomposed clinker throu...

Embodiment 3

[0047] 1. Preparation of bio-organic fertilizer 3

[0048] S1. Mix 50 parts of poultry manure, 35 parts of dregs, 20 parts of straw and 30 parts of bagasse, and then pulverize;

[0049] Livestock manure is a mixture of chicken manure and sheep manure, with a mass ratio of 1:1;

[0050] The dregs include 20 parts of ephedra, 35 parts of rhubarb, 20 parts of isatidis and 40 parts of rhubarb in terms of mass parts;

[0051] The straw is cowpea straw.

[0052] S2. Adjust the water content of the mixture pulverized material to 45%;

[0053] S3. Inoculate the composite bacterial liquid, stack retting into a conical shape for fermentation, turn over the fermented material when the temperature rises above 45°C during the stacking fermentation process, and ferment for 12 days to obtain decomposed clinker;

[0054] The inoculum amount of the compound bacteria solution is 1×10 5 ~1×10 6 cfu / g;

[0055] S4. Pass the decomposed clinker through a 40-mesh sieve to obtain a finished pow...

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Abstract

The invention relates to the technical field of agricultural soil bio-fertilizers, and particularly discloses a bio-organic fertilizer for enhancing disease resistance and stress resistance of crops and a preparation method thereof. The bio-organic fertilizer is prepared by inoculating raw materials of livestock and poultry manure, medicine residues, straws and bagasse with a compound bacteria solution composed of lactobacillus kitanensis, sinorhizobium meliloti and saccharomycetes, and then fermenting the raw materials of the livestock and poultry manure, the medicine residues, the straws and the bagasse. The fertilizer can be used for enhancing disease resistance and stress resistance of crops, and is beneficial to promoting improvement of planting soil and increasing crop yield.

Description

technical field [0001] The invention belongs to the technical field of agricultural soil biological fertilizers, in particular to a biological organic fertilizer for enhancing crop disease resistance and stress resistance and a preparation method thereof. Background technique [0002] Due to the unreasonable use of chemical fertilizers and chemical pesticides in agricultural production, soil compaction of cultivated land, low organic matter content, unbalanced crop nutrient absorption, reduced quality of crop products, reduced yield of cultivated land crops, enhanced resistance to diseases and insect pests, and excessive pesticide residues in agricultural products have caused a series of problems. These problems have brought challenges to the sustainable development of agriculture and ecological environmental protection. How to solve these problems has become a new topic for agricultural technicians. [0003] Bio-organic fertilizer is a new type of microbial organic compound...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/60C05G3/80C05G5/10C05F17/20C05F17/50
CPCC05F3/00C05F17/20C05F17/50C05G3/60C05G3/80C05G5/10C05F5/002C05F5/006C05F11/00C05F11/08Y02W30/40
Inventor 韦德枝王瑞兵
Owner 广西沃瑞德生物科技有限公司
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