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Method of precipitating phytase

A phytase and polyanion technology, applied in the field of concentration and compositions containing phytase, can solve the problem of low availability of non-ruminants, and achieve the effect of changing specific activity or yield, improving purity and simplifying production

Pending Publication Date: 2021-06-15
AB ENZYMES GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most (50-70%) dietary phosphate is bound to phytic acid and has low availability to non-ruminants

Method used

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  • Method of precipitating phytase
  • Method of precipitating phytase
  • Method of precipitating phytase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1 - Phytase Materials and Analytical Methods

[0090] The enzyme materials used for polyelectrolyte precipitation were different sources of phytase, namely E. coli phytase, Aspergillus phytase and Buttiauxella phytase. All these phytases are expressed in the Trichoderma reesei fungus. Precipitation of E. coli phytase or Aspergillus phytase was started using clarified spent broth from several different fermentations of phytase protein or their concentrates, which contained preservatives to prevent microbial contamination. The spent broth was clarified by filtration and concentrated using a 10 kDa ultrafiltration membrane to increase protein concentration. Precipitation of Buttiauxella phytase was performed using purified enzyme liquid from dried granules as starting material. The enzymatic activity of the protein was measured as the release of inorganic phosphate (FTU activity) from sodium phytate (0.98% (w / v) phytate) within 60 min at 37°C in 250 mM sodium a...

Embodiment 2

[0091] Example 2 - Reversible phytase-alginate complex formation by polyelectrolyte precipitation in a batch process

[0092] Four batches of precipitation were performed using a concentrate of E. coli phytase as the enzyme feedstock. Three of these batches were repeated batch precipitations in which buffered reagent solutions containing polyanions were prepared by adding 1 M sodium acetate buffer (nominal pH 3.6) and dry sodium alginate to tap water. In a batch precipitation, sodium citrate was used instead of sodium acetate as buffer. The dried alginate powder was dissolved and the reagent solution was stored overnight in a cold room. The next day, the phytase concentrate was added to the reagent solution at room temperature with proper stirring with a magnetic stirrer, following which the phytase-alginate complex formed rapidly. This was observed as a heavy, slightly fibrous and almost white precipitate formed during the phytase addition. Allow precipitation to continu...

Embodiment 3

[0096] Example 3 - Effect of Ionic Strength on Yield of Precipitated Phytase

[0097] The experiment was carried out using a concentrate of Escherichia coli phytase as the enzyme starting material. In order to study the effect of ionic strength and sodium alginate on the precipitation yield, a series of precipitation experiments were carried out at room temperature. The experiments were carried out in 15 ml test tubes and the total volume of the experiments was 10 ml. Reagent solutions were prepared by mixing 5M sodium chloride, 0.4% (w / w) sodium alginate and tap water in a test tube. Half of the experiments were performed with sodium alginate and the other half without sodium alginate. Add 2ml of phytase concentrate to the tube and mix immediately to allow complex formation. Experiments were performed without pH adjustment. The measured pH ranged from 4.3 to 4.7. Determine the resulting soluble and total protein concentrations. The final precipitation conditions and y...

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Abstract

Herein is disclosed a process for effectively precipitating phytase as a complex with a polyanion, as well as compositions comprising phytase and a polyanion, and a method for manufacturing such compositions.

Description

technical field [0001] The present invention relates to the field of protein production, in particular to the concentration of phytase and compositions comprising phytase. Background technique [0002] Phytases (phytate phosphate hydrolases; EC 3.1.3.8 and 3.1.3.26) are a class of enzymes found in plants, especially cereal and oil crop seeds, that catalyze phytic acid (phytic acid in the salt form Salt, also known as inositol polyphosphate, or phytate) hydrolyzed phosphatase. Most (50-70%) dietary phosphate is bound to phytic acid and has low availability to non-ruminants. [0003] Phytase is able to degrade phytate (phytate), resulting in the release of inorganic phosphorus. At the same time, compounds bound to the phytate are also released. Therefore, phytase can be used to increase the nutritional value of feed by releasing inorganic phosphate and other nutrients from phytate. In animal farming, supplementation of feed with phytase also helps to reduce the environment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16C12N9/00
CPCC12N9/00C12N9/16C12N9/96
Inventor 卡佳·帕尔穆宁米尔卡·帕卡莱宁莉娜·莱赫蒂卡里伊姆克·库恩
Owner AB ENZYMES GMBH
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