Albumin oil-in-water emulsion capable of generating flexible deformation as well as preparation method and application of albumin oil-in-water emulsion
An oil-in-water emulsion and albumin technology, applied in the field of biomedicine, can solve the problems of unsuitable use of ovalbumin emulsion, and achieve the effects of easy mass production, low cost and improving tissue penetration efficiency
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Embodiment 1
[0114] In this example, an oil-in-water emulsion is prepared based on albumin, and the specific preparation method is as follows:
[0115] (1) Albumin particle size distribution measurement:
[0116] Accurately weigh 8 mg of albumin (with a molecular weight of about 67 kilodaltons) using an electronic balance, dissolve it in 1 mL of PBS, and measure the average particle size of albumin by dynamic light scattering to be 7.4 nm;
[0117] Among them, the particle size distribution of albumin is shown in figure 1 shown.
[0118] (2) Preparation of albumin oil-in-water emulsion:
[0119] Accurately weigh 7.2 mg of albumin, add it to 900 μL of PBS, and disperse it evenly by ultrasonication for 1 min to obtain an aqueous phase suspension dispersed with albumin, and the pH value of the obtained aqueous phase suspension is 7.4;
[0120] Use a pipette gun to draw 100 μL of squalene and add it to the above aqueous suspension, and use ultrasonic (100W, 2min, super 4 seconds off for 4 s...
Embodiment 2
[0131] This example is used to detect the Young's modulus of the albumin oil-in-water emulsion. The specific method is as follows:
[0132] Use a hydrophobic pen to draw a circle on the positively charged glass slide, drop the emulsion prepared in Example 1 into the circle drawn on the glass slide, and after 2 minutes, rinse the excess emulsion with deionized water, and drop a drop to remove Ionized water on the surface of the emulsion;
[0133] Place the glass slide on the sample stage and adjust it to a suitable position. In the liquid environment, select the contact mode of the atomic force microscope. After finding the sample, select a suitable area to measure the indentation force curve.
[0134] The Young's modulus was calculated according to the Sneddon model.
[0135] The morphology of albumin oil-in-water emulsion before and after deformation is as follows: Figure 6 Shown; Young's modulus frequency distribution graph of albumin oil-in-water emulsion is shown in ...
Embodiment 3
[0137] This example is used to characterize the tissue penetration properties of deformable albumin oil-in-water emulsions. The specific method is as follows:
[0138] The mice were randomly divided into groups, and 6 mice were used in each group for the experiment, and the antigen was labeled with Cy5.
[0139] The albumin oil-in-water emulsion was prepared by the method described in Example 1, and the albumin particles were prepared by the method described in Comparative Example 1, and then intramuscularly injected after compounding with the antigen.
[0140] Take the injection site of the mouse at 6 hours and 18 hours (take the middle part of the injection site and the lymph node as much as possible), perform frozen section processing, and then perform immunofluorescence histochemistry to mark the cytoskeleton and lymphatic endothelial cytoskeleton, and observe the fluorescent antigen in the muscle tissue and distribution in lymphatic vessels.
[0141] Observe the section...
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