Selenium or thiothymine nucleoside-5 '-triphosphoric acid and synthesis method thereof
A technology of thiothymidine and thymidine, which is applied in the fields of modified triphosphate synthesis and nucleic acid molecule detection, can solve the problems of accurate detection by instruments, flooding, weak signal of low-copy nucleic acid molecules, etc., and achieves pairing accuracy The effect of improving, improving accuracy and sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0067] Example one
[0068] according to Figure 1-13 As shown, the present embodiment provides a selenate thymine-5'-triphosphate and a synthetic method thereof, including the following synthetic steps:
[0069] Step 1: 5'-DMTR-2-thio-thymidine is used as compound 1, with CH 3I alkylated 2-thiosyl functional group to activate 2-sulfur moiety to compound 2
[0070] Step 2: Compound 2 reacts with fresh prepared NaseH to complete selenium substitution, to obtain compound 3 at 80% yield
[0071] Step 3: Compound 3 is deprotected by trichloroacetic acid to obtain Se T, i.e., Compound 4
[0072] Step 4: Transform the compound 4SE by a pot synthesis Se TTP, i.e., 5SE
[0073] Step 5: After purifying the compound 5SE, it is characterized by nuclear magnetic resonance, mass spectrometry, and high performance liquid chromatography to confirm its structure and purity, then use Se TTP and DNA polymerase were used for DNA enzyme synthesis, and the DNA polymerase can be used in KleNow and TAQ. ...
Example Embodiment
[0098] Example 2
[0099] Such as figure 1 As shown, the present embodiment provides a thiosine thymidine nucleoside -5'-triphosphate synthesis method, including the following synthetic steps:
[0100] Step 1: The 5'-DMTR-2-thio thymidine is used as the starting reactant, i.e., Compound 1, and Trichloroacetic acid to remove the reaction. S T, i.e., Compound 4S;
[0101] Step 2: Transform compound 4S by a pot synthesis S TTP, ie, compound 5s;
[0102] Step 3: Purify the compound 5S to characterize it to confirm its structure and purity, then use S TTP and DNA polymerase for DNA enzyme synthesis; the present invention passes through innovative synthetic triphosphate, such as 2-thymidine nucleoside -5'-triphosphate ( S TTP) establishes a sulfur atom-specific modification strategy (SAM, Sulfur Atom-SpecificModification of Nucleic Acid) to inhibit T / g mismatch in DNA aggregation, increase the specificity of alkali-based parallelism, and demonstrate that the SAM method can increase po...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap