A kind of amphiphilic fluorescent conjugated polymer, preparation method and application

A conjugated polymer and amphiphilic technology, applied in the field of fluorescent sensing materials, to achieve good selectivity and high sensitivity, enhanced amphiphilicity, and strong interaction effects

Active Publication Date: 2022-05-17
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using multiple reactions to provide a novel structure of fluorescent conjugated polymers and mixed solvents, which can be used to construct a neomycin sensing system, to improve the expected sensitivity and detection effect has not been reported.

Method used

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  • A kind of amphiphilic fluorescent conjugated polymer, preparation method and application
  • A kind of amphiphilic fluorescent conjugated polymer, preparation method and application
  • A kind of amphiphilic fluorescent conjugated polymer, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 1. Synthesis of Monomers M1 and M2

[0050] (1) See attached figure 1 , which is a synthetic route diagram of the monomer M1 required for the preparation of fluorescent conjugated polymers. In this example, the monomer M1 is 1,4-diethynyl-2,5-didodecyloxybenzene.

[0051] figure 1 , the preparation of compound 1 (2,5-dibromohydroquinone): Weigh a certain amount of hydroquinone (16.201 g, 147 mmol) in a 500 mL double-necked flask, add acetic acid (82 mL) dissolve. Then measure 68 mL of acetic acid into the constant pressure dropping funnel, use a disposable medical syringe to draw 16 mL of liquid bromine and mix it with the acetic acid in the constant pressure dropping funnel. During the reaction, an ice-water bath was used, and the mixed solution of liquid bromine and acetic acid was slowly added dropwise, and the reaction was carried out overnight. After the reaction, the mixture in the flask was vacuum-filtered, and the solid product was recrystallized using met...

Embodiment 2

[0068] In this example, the photophysical properties of the polymer PFPE-COONa synthesized in Example 1 were measured. See attached Figure 9 , which is polymer PFPE-COONa in different THF / H 2 Fluorescence emission spectra in mixed solvents with O ratio. Depend on Figure 9 It can be seen that the polymer PFPE-COONa in different THF / H 2 The mixed solvent with O ratio has special photophysical properties and can be used in sensing and detection.

Embodiment 3

[0070] In this example, the polymer-conjugated polymer PFPE-COONa provided in Example 1 was used to detect neomycin.

[0071] Experimental method: Dissolving the polymer PFPE-COONa in THF / H 2 In O=1:1 mixed solvent, the concentration is 5×10-6 M (according to repeat unit), stir for 24 hours until the solution is stable; then, take 20 mL of the mixed solution in a 50 mL Erlenmeyer flask for detection experiments; configuration of the analyte solution: Neomycin is configured to a concentration of 1×10 -3 mol / L deionized aqueous solution; add a very small amount of neomycin solution (2 μL) into the Erlenmeyer flask each time, and calculate the concentration of neomycin in the solution to be tested; in actual detection, the set analyte The concentration gradient is 0, 0.1, 0.2, 0.3...1.6, 1.7 and 1.8 μM. Through this preparation method, the experimental error caused by the volume change of the solvent during the titration process can be ignored (the error range is less than 1%). ...

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Abstract

The invention relates to an amphiphilic fluorescent conjugated polymer, a preparation method and an application. The present invention adopts Sonogashira coupling and ester group hydrolysis reaction to obtain an amphiphilic conjugated macromolecule that contains both hydrophilic carboxylate groups and hydrophobic alkoxy side groups in a repeating unit, and dissolves it in In a mixed solvent with a volume ratio of tetrahydrofuran and water of 1:1, it is used for rapid sensing and detection of neomycin in water. The use of this amphiphilic fluorescent conjugated polymer can realize the specific detection of neomycin, and has a fluorescent color change that can be recognized by the naked eye, and the result can be obtained without the use of a large-scale instrument, and the operation is convenient. Realize more convenient, fast and efficient detection in practical application.

Description

technical field [0001] The invention relates to an amphiphilic fluorescent conjugated polymer containing both hydrophilic carboxylate groups and hydrophobic alkoxy side groups in repeating units, a preparation method and its sensing and detection for neomycin in aqueous solution. The invention belongs to the technical field of fluorescent sensing materials. Background technique [0002] In 1949, neomycin, a multifunctional broad-spectrum antibacterial antibiotic discovered for the first time by Waksman and Lechevalier, was widely used as a veterinary drug to treat bacterial infections among animals (see literature: Science 1949, 109 , 305-307; Spectrochim. Acta A 2018, 190 , 268-273). Neomycin has good activity against Gram-negative bacteria and is also important for inhibiting the growth of Gram-positive bacteria (see literature: Biosens. Bioelectron. 2010, 26 , 1002-1008; Macromol. Rapid Commum. 2013, 34 , 944-948). Because of its low cost and high treatment ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G61/02C09K11/06G01N21/64
CPCC08G61/02C09K11/06G01N21/6428C09K2211/1416G01N2021/6417C08G2261/124C08G2261/1426C08G2261/1424C08G2261/18C08G2261/3142C08G2261/312C08G2261/354C08G2261/364C08G2261/413C08G2261/522C08G2261/94Y02E10/549
Inventor 范丽娟陈嘉骏
Owner SUZHOU UNIV
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