Method for screening hypoglycemic peptide for inhibiting activity of alpha-amylase

Abstract

The invention discloses a method for screening a hypoglycemic peptide for inhibiting activity of alpha-amylase, and belongs to the field of preparation and purification of bioactive peptides. According to the method, the tea seed meal proteolysis alpha-amylase activity inhibiting hypoglycemic peptide is rapidly enriched, separated and purified by combining magnetic separation of the magnetic immobilized alpha-amylase with a high performance liquid chromatography technology. The magnetic immobilized alpha-amylase is adopted to enrich the hypoglycemic peptide for inhibiting the activity of the alpha-amylase, the invention has the advantages of good specificity, rapidness and high efficiency, the magnetic enzyme can be reused, and another technical support is provided for quantitative detection of the hypoglycemic peptide. The cost is low, the operation is simple, and the practicability is very high. The new source of the hypoglycemic active peptide is increased, the full utilization of byproducts is realized, and the method has great potential in the aspect of developing functional food with hypoglycemic activity.

Description

technical field [0001] The invention relates to a method for screening a hypoglycemic peptide that inhibits alpha-amylase activity, and belongs to the field of bioactive peptide preparation. Background technique [0002] α-amylase (1,4-α-D-glucan-glucanohydrolase) is a very important enzyme in the digestion process of carbohydrates in food. By inhibiting its activity, glucose and maltose The production and absorption of the drug are slowed down to achieve the purpose of lowering postprandial blood sugar, thereby reducing the damage to the body of diabetic patients in a high-sugar environment, and effectively preventing and improving complications. α-amylase inhibitors can strictly control the rise of postprandial blood sugar, and are an important means of treating type 2 diabetes; a large number of studies have shown that glycosidase inhibitors play an important role in the treatment and prevention of diabetes. [0003] Bioactive peptides have a variety of biological functi...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to address the shortcomings of screening active ingredients from complex compounds, and propose a method for enriching and screening hypoglycemic peptides using magnetically immobilized amylase combined with high-performance liquid phase technology, which can increase the number of anti-α-amylase-activating hypoglycemic peptides screened and targeting, to achieve efficient and specific separation and detection of trace blood sugar-lowering active ingredients; using the prepared magnetically immobilized amylase, it can quickly and effectively screen hypoglycemic peptides that inhibit α-amylase activity, which is more effective for magnetic enzymes Provide technical support for the ground isolation of hypoglycemic active ingredients

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