Biomass carbon dot fluorescent probe for ratio quantitative detection of adriamycin as well as preparation method and application of biomass carbon dot fluorescent probe

A biomass carbon and fluorescent probe technology, applied in the field of fluorescent probes, can solve the problems of complicated preparation process, poor anti-interference ability and high technical requirements, and achieve the effect of simple preparation method, strong anti-interference ability and low preparation cost

Active Publication Date: 2021-06-29
SHANXI UNIV
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Problems solved by technology

These methods have unique advantages, but also have the following defects: complex test conditions, tedious and time-consuming, expensive instruments and equipment, long detection cycle, high technical requirements, high cost, poor anti-interference ability and low accuracy, etc., resulting in these The detection method cannot be widely used
However, the addition of ethylenediamine makes the preparation process too complicated, and the properties of carbon quantum dots prepared by pure corncobs cannot be obtained, which leads to the limitation of corncobs in the field of carbon quantum dot preparation and the application of the obtained carbon quantum dots. Therefore, a single corncob It is of great significance to prepare carbon quantum dots for carbon sources and study the properties and applications of carbon quantum dots in detail

Method used

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  • Biomass carbon dot fluorescent probe for ratio quantitative detection of adriamycin as well as preparation method and application of biomass carbon dot fluorescent probe
  • Biomass carbon dot fluorescent probe for ratio quantitative detection of adriamycin as well as preparation method and application of biomass carbon dot fluorescent probe
  • Biomass carbon dot fluorescent probe for ratio quantitative detection of adriamycin as well as preparation method and application of biomass carbon dot fluorescent probe

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation and characterization of biomass carbon dots

[0033] Step 1: Weigh 0.5 g of corncob granular powder, add 10 mL of ultrapure water to it, and heat and carbonize in a high-pressure reactor at 180 °C for 12 h to obtain a light yellow biomass carbon dot solution.

[0034] Step 2: After the reactor is cooled to room temperature, the light yellow solution is centrifuged at 8000 rpm for 10 minutes to obtain a clear solution of biomass carbon dots, which is then freeze-dried to obtain solid biomass carbon dot powder.

[0035] Step 3: Accurately weigh 0.1 g of biomass carbon dot solid powder into a beaker, add 10 mL of ultrapure water to it, stir to dissolve it fully, and obtain a biomass carbon dot stock solution with a concentration of 10 mg / mL.

[0036] Characterization see figure 1 with figure 2 . figure 1 In the ultraviolet-visible absorption spectrum of biomass carbon dots, there are two obvious absorption peaks located at 281 nm and 323 nm, whic...

Embodiment 2

[0037] Embodiment 2: Anti-interference experiment of doxorubicin detection

[0038] Step 1: Weigh drugs of different qualities (biotin, amoxicillin, lincomycin, penicillin G sodium, kanamycin, thiamine, ascorbic acid), add 10 mL of ultrapure water, and prepare a concentration of 0.01 mol / L drug stock solution.

[0039] Step 2, weighing amino acids of different masses (methionine, asparagine, glutamic acid, tyrosine, proline, isoleucine, phenylalanine, leucine, threonine, alanine, cysteine, serine, glycine, lysine, arginine, valine, histidine, glutamine, homocysteine), add 10 mL of ultrapure water to make up the concentration 0.01 mol / L amino acid stock solution.

[0040] Step 3, measure the fluorescence intensity of 0.148 mg / mL biomass carbon dot solution (2030 μL), denoted as F 0 ; Add 100 μL drug stock solution to it, the concentration of the drug at this time is 469 μmol / L, measure the fluorescence intensity of the solution at this time, denoted as F 1 ; add 10 µL doxor...

Embodiment 3

[0043] Embodiment 3: the linear equation of doxorubicin titration biomass carbon point

[0044] Step 1, measure the fluorescence intensity of 0.148 mg / mL biomass carbon dots, denoted as I 437 and I 553 .

[0045] Step 2, add doxorubicin stock solution dropwise to the above solution, and record the fluorescence intensity I 437 and I 553 , the fluorescence intensity changes see Figure 8 .

[0046] Step 3, using Origin software, fitting the change of fluorescence intensity (I 555 / I 435 ) and the linear equation between the concentration of doxorubicin, see Figure 9 with Figure 10 .

[0047] Figure 8 It shows that with the addition of doxorubicin stock solution, the fluorescence at 435 nm of the mixed solution of biomass carbon dots and doxorubicin is gradually quenched, and the fluorescence at 553 nm is gradually enhanced. Figure 9 , Figure 10 It is a linear relationship diagram between the change value of fluorescence intensity of biomass carbon dots and doxor...

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Abstract

The invention belongs to the technical field of fluorescent probes, and provides a biomass carbon dot fluorescent probe for ratio quantitative detection of adriamycin as well as a preparation method and application of the biomass carbon dot fluorescent probe. The method comprises the following steps: with 150-mesh corncob powder as a carbon source, a nitrogen source, a sulfur source and a phosphorus source, heating and carbonizing the 150-mesh corncob powder in a high-pressure reaction kettle at 180 DEG C for 12 hours to prepare a biomass carbon dot solution; and conducting centrifuging to remove insoluble substances, and performing freeze-drying to obtain biomass carbon dot solid powder. According to the invention, a linear relation between the concentration of adriamycin and the fluorescence intensity of a biomass carbon dot are determined by utilizing a ratio fluorescence detection method; and the content of adriamycin in an actual urine sample and the adding standard recovery rate of adriamycin are detected by utilizing a constructed linear equation. The method is simple and convenient to operate, strong in anti-interference performance, free of expensive instruments and equipment and low in detection cost, has self-calibration performance, can rapidly, efficiently, sensitively and quantitatively detect adriamycin in an actual sample, and has good reproducibility.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, and in particular relates to a biomass carbon dot fluorescent probe for ratiometric and quantitative detection of doxorubicin, a preparation method and application thereof. A biomass carbon dot prepared by a simple and rapid method and used for ratiometric quantitative detection of doxorubicin. Background technique [0002] Doxorubicin, also known as doxorubicin. It is an antibiotic that can be used to fight against tumors. It has inhibitory effects on the synthesis of RNA and DNA. Among them, it has the strongest inhibitory effect on RNA and has a wide anti-tumor spectrum. It is a cycle non-specific drug. All have a killing effect. It is suitable for a variety of diseases, such as acute leukemia, malignant lymphoma, breast cancer, bronchial lung cancer, ovarian cancer, gastric cancer, liver cancer, etc. [0003] The methods that have been used to detect doxorubicin at present inclu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C01B32/15B82Y40/00B82Y20/00G01N21/64
CPCC09K11/65G01N21/6428B82Y20/00B82Y40/00C01B32/15G01N2021/6432
Inventor 弓晓娟方晓彤董文娟宋胜梅董川
Owner SHANXI UNIV
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