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South America white pair DNA binding antibacterial peptide VPDB40 and application thereof

A technology of antimicrobial peptides and white prawns, applied in the fields of application, antibacterial drugs, peptides, etc., can solve the problems that there is no nucleic acid sequence, recombinant protein research and application, etc.

Active Publication Date: 2021-07-09
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In Penaeus vannamei or other crustaceans, many antimicrobial peptide genes have been studied and reported, but the present invention has discovered a novel DNA-binding antimicrobial peptide during the research process, and there is no research on its nucleic acid sequence, recombinant protein and Application related reports

Method used

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  • South America white pair DNA binding antibacterial peptide VPDB40 and application thereof
  • South America white pair DNA binding antibacterial peptide VPDB40 and application thereof
  • South America white pair DNA binding antibacterial peptide VPDB40 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Mass spectrometric analysis of Penaeus vannamei DNA-binding antimicrobial peptides

[0042] Firstly, the samples of Penaeus vannamei were prepared, and 400 healthy Penaeus vannamei were divided into high-intensity ultrasonic group, medium-intensity ultrasonic group, low-intensity ultrasonic group and control group, 100 each. Each shrimp was injected with 0.1mL Vibrio parahaemolyticus (1.0×10 5-6 cfu / mL) or an equivalent amount of sodium chloride. Live shrimp were collected 24 hours after infection and minced immediately with PBS buffer. After centrifuging 100 g for 15 min, the supernatant was taken and passed through a 3000 Da filter membrane. Store at -20°C for further analysis.

[0043] This was then performed using a chromatographic Nano Aquity UPLC system (Waters Corp.) instrument. The injection volume was 5.0 μl, and the mobile phases were 0.1% aqueous methanol and acetonitrile, respectively. The elution gradient is shown in Table 1.

[0044] Table ...

Embodiment 2

[0062] Embodiment 2 Minimum Inhibitory Concentration (MIC) Determination

[0063] Cultivate Vibrio parahaemolyticus at 37°C for 12h to logarithmic growth phase, dilute to 10 in 0.01M pH 7.2 phosphate buffer 6-7 CFU / mL. Dissolve the peptide in phosphate buffer, and mix it with bacteria in an equal volume at 37°C for 2 hours. The minimum inhibitory concentration (MIC) refers to the lowest concentration of antimicrobial peptide at which no bacterial growth can be seen from the microtiter plate after incubation at 37°C. like figure 2 As shown, the minimum inhibitory concentration (MIC) of VPDB40 to Vibrio parahaemolyticus was 1.95 μg / mL.

Embodiment 3

[0064] Embodiment 3 time-killing curve (Time kill) is measured

[0065] The time-kill curve of peptide VPDB40 was determined using the plate count method. Cultivate Vibrio parahaemolyticus at 37°C for 12h to logarithmic growth phase, dilute to 10 in 0.01M pH 7.2 phosphate buffer 6-7 CFU / mL. The peptide was dissolved in phosphate buffer and diluted to 3.91 μg / mL, mixed with bacteria in equal volume, and incubated at 37°C. At different time points (ie, 0.5, 1, 1.5, 2, 2.5 and 3 o'clock), 0.2 mL of bacterial suspension was obtained, and colonies were counted after 24 hours of culture on a nutrient broth plate at 37°C. like image 3 shown.

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Abstract

The invention discloses a penaeus vannamei DNA combined antibacterial peptide VPDB40, wherein the amino acid sequence of the penaeus vannamei DNA combined antibacterial peptide VPDB40 is GITIQCILPGFVVSKLSKLK, and the molecular weight of the antibacterial peptide is 2144.686 Daltons. The antibacterial peptide can effectively inhibit the growth of vibrio parahaemolyticus, and can be used for preparing drugs for preventing or inhibiting vibrio parahaemolyticus infection. The invention lays a foundation for further research on the development of the penaeus vannamei antibacterial peptide as a food preservative and a feed additive for preventing penaeus vannamei diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a DNA-binding antibacterial peptide VPDB40 of Penaeus vannamei and its application. Background technique [0002] Penaeus vannamei, as the main species of shrimp cultured in my country, has high economic value. In recent years, due to the deterioration of the breeding environment and the excessive breeding density, the frequent occurrence of diseases has seriously threatened the healthy development of the vannamei industry and caused huge economic losses. Therefore, it is particularly important to carry out the prevention and control of shrimp diseases. [0003] In recent years, a mysterious disease called "Shrimp Early Mortality Syndrome" has caused a large number of farmed shrimp deaths in many Asian countries. It has been confirmed that the culprit is Vibrio parahaemolyticus. The bacteria are transmitted orally and colonized in the digestive tract of prawns to produce toxins that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/04A23K50/80A23K20/147
CPCC07K7/08A61P31/04A23K50/80A23K20/147A61K38/00
Inventor 杨燊王铭遥黄世英郑明静石林凡翁武银
Owner JIMEI UNIV
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