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Extraction process of tea bran

An extraction process and technology of tea bran, applied in the field of extraction of plant active ingredients, can solve the problem of low extraction efficiency, and achieve the effects of increasing extraction efficiency, promoting flocculation and sedimentation, and improving extraction efficiency and purity.

Active Publication Date: 2021-07-09
广州芊亮科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction efficiency of the existing technology is not high, so it is of great significance to study a high-efficiency extraction method to improve the extraction efficiency and purity of tea saponin and tea polysaccharides in tea bran

Method used

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  • Extraction process of tea bran

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A process for extracting tea bran, comprising the following steps:

[0040] (1) tea bran is obtained by oil-pressing tea seeds, and tea bran powder is obtained by pulverizing the tea bran;

[0041] (2) Disperse 300g of tea bran powder with water, use 0.3% cellulase (enzyme activity of 20000U / g) and 0.03% pectinase (enzyme activity of 25000U / g) to enzymolyze, filter, add to the filtrate 0.6% mass (based on the mass of tea bran powder) of extractant (a mixture of sodium carbonate and sodium salicylate with a mass ratio of 3:1), centrifuged to obtain supernatant 1 and precipitate 1;

[0042] (3) adding the precipitate 1 to 6 times the mass of 70% ethanol aqueous solution for leaching, and centrifuging to obtain supernatant 2 and precipitate 2;

[0043] (4) After supernatant 1 and supernatant 2 are mixed, add 0.3% (in terms of tea bran powder mass) of precipitant (mass ratio is 2:1 mixture of sodium alginate and chitosan, wherein, the Described sodium alginate is added wi...

Embodiment 2

[0047] A process for extracting tea bran, comprising the following steps:

[0048] (1) tea bran is obtained by oil-pressing tea seeds, and tea bran powder is obtained by pulverizing the tea bran;

[0049] (2) Disperse 300g of tea bran powder with water, use 0.1% cellulase (enzyme activity of 25000U / g) and 0.05% pectinase (enzyme activity of 50000U / g) to enzymolyze, filter, add to the filtrate 1% mass (based on the mass of tea bran powder) of extractant (a mixture of sodium carbonate and sodium salicylate with a mass ratio of 1:1), centrifuged to obtain supernatant 1 and precipitate 1;

[0050](3) adding the precipitate 1 to 10 times the mass of 60% ethanol aqueous solution for leaching, and centrifuging to obtain supernatant 2 and precipitate 2;

[0051] (4) After supernatant 1 and supernatant 2 are mixed, add 0.5% (in terms of tea bran powder mass) of precipitant (mass ratio is 1:1 mixture of sodium alginate and chitosan, wherein, the Described sodium alginate is added with...

Embodiment 3

[0055] A process for extracting tea bran, comprising the following steps:

[0056] (1) tea bran is obtained by oil-pressing tea seeds, and tea bran powder is obtained by pulverizing the tea bran;

[0057] (2) Disperse 300g of tea bran powder with water, use 0.5% cellulase (enzyme activity is 50000U / g) and 0.01% pectinase (enzyme activity is 20000U / g) after enzymolysis, filter, add to the filtrate 0.5% mass (based on the mass of tea bran powder) of extractant (a mixture of sodium carbonate and sodium salicylate with a mass ratio of 5:1), centrifuged to obtain supernatant 1 and precipitate 1;

[0058] (3) adding the precipitate 1 to 5 times the mass of 75% ethanol aqueous solution for leaching, and centrifuging to obtain supernatant 2 and precipitate 2;

[0059] (4) After the supernatant 1 and the supernatant 2 are mixed, add 0.2% (in terms of tea bran powder mass) of a precipitant (mass ratio is a mixture of sodium alginate and chitosan at 3:1, wherein the Described sodium al...

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Abstract

The invention belongs to the technical field of extraction of effective components of plants, and particularly discloses an extraction process of tea bran. The extraction method of the tea bran comprises the steps of enzymolysis of the tea bran, extraction and separation of effective components, purification of tea polysaccharide and tea saponin, and the like. According to the process, the tea polysaccharide and the tea saponin in the tea bran can be efficiently extracted, and the extraction efficiency and the purity of the tea saponin and the tea polysaccharide in the tea bran are effectively improved.

Description

technical field [0001] The invention belongs to the technical field of extraction of plant effective components, and in particular relates to an extraction process of tea bran. Background technique [0002] Tea bran is tea seed cake, also called tea dry, which is the residue cake of camellia oleifera seed after oil extraction. Tea bran contains tea saponin, protein, oil content, natural tea polyphenols, and is a natural cleaning product. [0003] Tea saponin is also called tea saponin, tea saponin, a class of saponins. Studies have found that tea seed saponin has hemolysis and fish poisoning, anti-insect and bactericidal effects, anti-osmosis and anti-inflammation, phlegm and cough, analgesic, anti-cancer and other pharmacological functions. Tea seed saponin is also an excellent natural nonionic surfactant with strong foaming, emulsifying, dispersing, and moisturizing effects. The tea seed meal left after the oil camellia seed is pressed contains 10% to 14% tea saponin. T...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07H15/256C07H1/08C07J63/00
CPCC07H1/08C07H15/256C07J63/008C07K1/145C07K1/30C08B37/0003
Inventor 黄良蔡波杨志带
Owner 广州芊亮科技有限公司
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