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Preparation method of antioxidant hemostatic sponge with high imbibition rate

A hemostatic sponge and anti-oxidation technology, which can be used in medical formulations, surgery, drug delivery, etc., and can solve problems such as unfavorable hemostasis, low liquid absorption rate, and general mechanical strength of the sponge

Active Publication Date: 2021-07-09
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lan et al. added tannic acid to the mixing process of chitosan and gelatin for cross-linking, which improved the strength of the sponge, and the hemostatic performance of the sponge was further improved [Colloids and Surfaces B: Biointerfaces 136(2015) 1026–1034] , the defect of the method is that tannic acid is added under acidic conditions, only the hydrogen bond cross-linking of tannic acid and chitosan / gelatin can occur, and the chemical cross-linking-Schiff base reaction cannot occur, so that the mechanical strength of the sponge is general , the liquid absorption rate is not high (<25 times), which is not conducive to hemostasis; in addition, no attention has been paid to the influence of the rotation speed of the stirring process on the performance of the gelatin-based sponge

Method used

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  • Preparation method of antioxidant hemostatic sponge with high imbibition rate
  • Preparation method of antioxidant hemostatic sponge with high imbibition rate

Examples

Experimental program
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Effect test

Embodiment 1

[0021] 1) Weigh 1g gelatin and dissolve it in 50mL deionized water; weigh 1g chitosan and dissolve it in 50mL acetic acid / water mixed solution. Mix the gelatin and chitosan solution at a mass ratio of 1:9 in a 40-degree water bath, then stir at a low speed of 200rpm for 10 minutes for mixing and crosslinking, then increase the speed to 1100rpm for foaming, and pour it into a petri dish after foaming for 35 minutes. Freeze and then freeze dry. The freeze-dried sponge was washed with acetic acid with ethanol, and after several washes, it was vacuum-dried.

[0022] 2) Dissolve tannic acid in Tris buffer solution (pH=9) at a concentration of 5 mg / mL, soak the sponge obtained above in tannic acid for 15 minutes, then take out the sponge for washing, wash with deionized water three times, freeze at low temperature, Then a second freeze-drying was performed.

Embodiment 2

[0024] 1) Weigh 1g gelatin and dissolve it in 50mL deionized water; weigh 1g chitosan and dissolve it in 50mL acetic acid / water mixed solution. Mix the gelatin and chitosan solution at a mass ratio of 3:7 in a 40-degree water bath, then stir at a low speed of 200rpm for 15 minutes for mixing and cross-linking, then increase the speed to 1300rpm for foaming, and pour it into a petri dish after foaming for 25 minutes. Freeze and then freeze dry. The freeze-dried sponge was washed with acetic acid with ethanol, and after several washes, it was vacuum-dried.

[0025] 2) Dissolve proanthocyanidins in Tris buffer (pH=8.5) at a concentration of 3 mg / mL, soak the sponge obtained above for 0 min, then take out the sponge for washing, wash with deionized water three times, freeze at low temperature, and then carry out Secondary freeze-drying.

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Abstract

The invention relates to a preparation method of antioxidant hemostatic sponge with high imbibition rate, which comprises the following steps: preparing an acetic acid solution of chitosan, dissolving gelatin in hot water, stirring at a low speed to carry out mixed crosslinking, increasing the rotating speed to carry out high-speed foaming, and stopping foaming until uniform and stable bubbles are obtained; pouring the foaming liquid into a mold, performing low-temperature freeze drying to obtain foamed sponge, soaking the foamed sponge in an absolute ethyl alcohol solution to wash acetic acid, and performing vacuum drying; wherein the mass ratio of the gelatin to the chitosan is 1: (1.5-10); preparing a natural polyphenol alkaline solution with the concentration of 1-10mg / mL, adjusting the pH value to 8-10, soaking the foamed sponge subjected to vacuum drying in the natural polyphenol alkaline solution for crosslinking, taking out the foamed sponge, washing with deionized water, freezing the washed foamed sponge at low temperature, and carrying out secondary freeze drying. The antioxidant hemostatic material with excellent hemostatic performance, good biocompatibility and good oxidation resistance is obtained.

Description

technical field [0001] The invention belongs to the field of medical hemostatic materials, and relates to a preparation method of a hemostatic sponge with high anti-oxidation and high liquid absorption rate. Background technique [0002] Uncontrollable bleeding is a life-threatening threat to people's lives, and it is also one of the challenges faced by hospital emergency personnel. Therefore, it is of great practical significance to prepare materials with safe and efficient hemostatic properties. Gelatin has the advantages of low antigenicity, good biocompatibility, good biodegradability, and relatively low cost. It has received extensive attention as a hemostatic material. However, gelatin products have poor mechanical strength and need to be compounded with other materials with high mechanical strength. Or increase its strength by cross-linking. The common gelatin sponges on the market, represented by Jinling Sponge, are usually cross-linked by formaldehyde, glutaraldehy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/40C08J9/28C08J3/24C08L5/08C08L89/00A61L24/00A61L24/08A61L24/10
CPCC08J9/40C08J9/28C08J3/246A61L24/0036A61L24/08A61L24/104A61L24/0015A61L24/001C08J2305/08C08J2389/00A61L2400/04A61L2300/216A61L2300/412C08L5/08C08L89/00
Inventor 徐福建胡杨缪腾飞
Owner BEIJING UNIV OF CHEM TECH