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Application of cyclocarya paliurus extract in increasing fat-reducing gene expression quantity, increasing basal metabolic rate and/or inhibiting fat accumulation

A technology of basal metabolic rate and gene expression, applied in the field of Cyclocarya paliurus extract, can solve the problems of not widely understood and applied, and scarce distribution

Inactive Publication Date: 2021-07-20
百岳特生物技术(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The scientific name of Cyclocarya paliurus is Cyclocarya paliurus, also known as the money tree. It is a rare tree species left over from the Ice Age in the fourth season. Scientists are still very puzzled why Cyclocarya paliurus survived the Ice Age. Willow is only scattered in a few areas of China, and it is listed as the second-level national protected tree species by China. Its ingredients are ancient and its distribution is very rare, so it has the reputation of "the giant panda in the plant kingdom". However, its ingredients are effective and practical. Uses are not yet widely understood and applied

Method used

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  • Application of cyclocarya paliurus extract in increasing fat-reducing gene expression quantity, increasing basal metabolic rate and/or inhibiting fat accumulation
  • Application of cyclocarya paliurus extract in increasing fat-reducing gene expression quantity, increasing basal metabolic rate and/or inhibiting fat accumulation
  • Application of cyclocarya paliurus extract in increasing fat-reducing gene expression quantity, increasing basal metabolic rate and/or inhibiting fat accumulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1. The preparation method of Cyclocarya paliurus extract

[0071] In this embodiment, the preparation steps of Cyclocarya paliurus extract are as follows:

[0072] 1. First, carry out the crushing procedure. The leaves of Cyclocarya paliurus (Origin: China) were coarsely crushed (Osterizer brand 10speed blender) and sieved with a sieve with a hole diameter of 12 mm to remove oversized particles to obtain Cyclocarya paliurus coarse crushes.

[0073] 2. Next, in the heating process, water is used as a solvent and the coarse crushed Cyclocarya paliurus is mixed at a weight ratio of 30:1 (liquid-solid ratio), and extracted at 85±5°C for about 60 minutes to form an extract. In this example, the weight ratio of the solvent to the coarse crushed Cyclocarya paliurus is 20:1, the temperature during soaking is 80° C., and the extraction time is 60 minutes. If the solvent is too small or the extraction time is too short, the extraction efficiency will be significantly...

Embodiment 2

[0079] Embodiment 2. Cell basal metabolic rate test

[0080] The basal metabolic rate refers to the energy consumed by the body to maintain basic functions. The higher the basal metabolic rate, the more energy the body consumes, and the easier it is to have a body that is easy to lose weight. That is, increasing the basal metabolic rate can effectively help prevent and improve obesity.

[0081] In order to investigate the effect of Cyclocarya paliurus extract on the basal metabolic rate of cells, this example uses the amount of the final product of metabolism, pyruvate (Pyruvate), to determine the basis of mouse myoblast C2C12 treated with Cyclocarya paliurus extract. Changes in metabolic rate.

[0082] Materials and Instruments

[0083] 1. Cell line: mouse myoblast C2C12, obtained from BCRC; Cat.60083.

[0084] 2. Culture medium: Dulbecco's Modified Eagle's Medium (DMEM, purchased from Gibco, 12100-046) was added with additional components to make it contain 10vol% FBS (fet...

Embodiment 3

[0109] Example 3. Cyclocarya paliurus extract inhibits fat accumulation efficacy test

[0110] Fat is stored in fat cells in the form of oil droplets. Based on this, this test analyzes the stained oil droplets to observe the number of oil droplets in the cells, so as to confirm the state of fat accumulation. Subsequently, the dye is dissolved and analyzed as a quantitative numerical index.

[0111] In this experiment, mouse bone marrow stromal cells (hereinafter referred to as OP9 cells) were used. OP9 cells were purchased from the American Type Culture Collection (American Type Culture Collection, ) OP9 cell line (ATCC CRL-2749).

[0112] First, take a 24-well culture plate and inoculate each well with 8×10 4 OP9 cells and 500 μL medium (Medium), the medium is MEMAM (Minimum Essential Medium Alpha Medium, purchased from Gibco, product number Cat. , purchased from Gibco, product number Cat.10437-028) and 0.1% penicillin / streptomycin (Penicillin-streptomycin, purchased fro...

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Abstract

The invention provides application of a cyclocarya paliurus extract to preparation of a composition for increasing fat-reducing gene expression quantity, increasing basal metabolic rate and / or inhibiting fat accumulation. The cyclocarya paliurus extract can effectively promote activity of metabolic fat and increase pyruvic acid generation of muscle cells, reduces accumulation of fat oil drops in fat cells, further achieves the effects of reducing body weight, whole body fat, trunk body fat and waistline, and is obtained by extracting cyclocarya paliurus with a solvent, which is a water-containing solvent.

Description

technical field [0001] The present invention relates to the use of Cyclocarya paliurus extract, in particular to the use for preparing a composition for improving fat-reducing gene expression, increasing basal metabolic rate and / or inhibiting fat accumulation. Background technique [0002] In recent years, the incidence of obesity has increased year by year and has reached a global epidemic trend, and is regarded as a chronic disease. As of 2016, the World Health Organization estimated that 39% of the population was overweight (about 1.9 billion people), and about 13% of the population is obese (approximately 600 million people), and published data in 2019 indicated that a total of 38 million children under the age of 5 were overweight or obese. [0003] Obese patients are often accompanied by other health problems and lead to increased risk of related diseases, including: insulin resistance, type 2 diabetes, fatty liver, cardiovascular disease, obstructive apnea, high blood...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61P3/04A23L33/105
CPCA61P3/04A61K36/288A61P3/06A61P21/00A61P7/10A23L33/105A23V2002/00A23V2200/332A23V2200/3262A23V2250/21
Inventor 林咏翔李姿仪
Owner 百岳特生物技术(上海)有限公司
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