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Glucose-inhibiting type oxygen-resistant hexanoic acid producing bacterium

A technology for glucose inhibition and caproic acid bacteria, which is applied to bacteria, microorganisms, biofuels, etc., can solve the problems of unfavorable industrial cultivation and application of caproic acid bacteria, and achieve the effects of improving wine quality, simple cultivation conditions and strong oxygen resistance.

Active Publication Date: 2021-07-23
HUBEI UNIV OF TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the currently disclosed caproic acid bacteria have relatively strict cultivation conditions and need to be cultivated in special anaerobic incubators or anaerobic tanks, which is not conducive to the industrial cultivation and application of caproic acid bacteria

Method used

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  • Glucose-inhibiting type oxygen-resistant hexanoic acid producing bacterium
  • Glucose-inhibiting type oxygen-resistant hexanoic acid producing bacterium
  • Glucose-inhibiting type oxygen-resistant hexanoic acid producing bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Strain screening of glucose-inhibiting oxygen-tolerant caproic acid bacteria of the present invention

[0034] The glucose-inhibiting oxygen-tolerant caproic acid bacteria (Clostridium celerecrescens) of the present invention is isolated from high-quality old pit mud of a certain winery in Hubei, using ES (ethanol sodium acetate) medium: yeast extract 1%, anhydrous sodium acetate 0.5%, MgSO 4 ·7H 2 O 0.02%, K 2 HPO 4 0.04%, (NH 4 ) 2 SO 4 0.05%, after sterilization, add 2% absolute ethanol and 1% calcium carbonate. Take an appropriate amount of pit mud and add it into a test tube with 90% ES medium, place it in a 35°C incubator and culture it for 7 days, observe the gas production, put the test tube with good gas production in a water bath at 80°C for 10 minutes, Then, 1 mL of the bacterial solution was added into test tubes containing ES medium for enrichment culture, and repeated 4 times. Then use the copper sulfate chromogenic method to qualitative...

Embodiment 2

[0037] Example 2 Verification of the amount of caproic acid produced by the glucose-inhibiting type oxygen-tolerant caproic acid bacteria of the present invention

[0038] Glucose-inhibiting type oxygen-tolerant caproic acid bacteria of the present invention is inoculated in the 100mL stopper test tube that ES substratum is housed with 5% inoculum (ES substratum composition: yeast extract 1%, anhydrous sodium acetate 0.5%, MgSO 4 ·7H 2 O 0.02%, K 2 HPO 4 0.04%, (NH 4 ) 2 SO 4 0.05%, after sterilization, add 2% absolute ethanol and 1% calcium carbonate. ), the liquid content is more than 90%, and then sealed with a parafilm. Incubate at 33°C for 7 days and use as seed solution. Inoculate 5% of the seed solution into a 100mL stoppered test tube filled with culture medium, the filling volume is more than 90%, and then seal it with a parafilm. Cultivate at a constant temperature at 33°C, and start sampling every day from the third day to detect the content of butyric aci...

Embodiment 3

[0043] Example 3 Verifying the Effects of Different Temperatures on the Glucose-inhibiting Oxygen-tolerant Caproic Acid Bacteria of the Present Invention

[0044] Glucose-inhibiting type oxygen-tolerant caproic acid bacteria of the present invention is inoculated in the 100mL stopper test tube that ES substratum is housed with 5% inoculum size (ES substratum composition: yeast extract 1%, anhydrous sodium acetate 0.5%, MgSO 4 ·7H 2 O 0.02%, K 2 HPO 4 0.04%, (NH 4 ) 2 SO 4 0.05%, after sterilization, add 2% absolute ethanol and 1% calcium carbonate. ), the liquid content is more than 90%, and then sealed with a parafilm. Incubate at 33°C for 7 days and use as seed solution. Inoculate 5% of the seed solution into a 100mL stoppered test tube filled with culture medium, the filling volume is more than 90%, and then seal it with a parafilm. Culture at constant temperature at 33°C, 34°C, 35°C, and 36°C, respectively, and take samples during the fermentation process to dete...

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Abstract

The invention relates to the technical fields of wine-brewing microbes and brewage and particularly relates to a glucose-inhibiting type oxygen-resistant hexanoic acid producing bacterium. The strain has been collected in China Center for Type Culture Collection (CCTCC) and has an accession number of CCTCCM 2020881. The glucose-inhibiting type oxygen-resistant hexanoic acid producing bacterium disclosed by the invention has the characteristics of oxygen resistance and high hexanoic acid yield, the hexanoic acid producing capability will be inhibited in a high-glucose culture medium, and the hexanoic acid producing inhibiting effect will be higher along with increase of a glucose concentration when the culture medium simultaneously contains glucose and ethanol and the glucose content is higher than 0.5%. The hexanoic acid producing capability of the glucose-inhibiting type oxygen-resistant hexanoic acid producing bacterium disclosed by the invention can reach 10.61g / L, the oxygen resistance is relatively good, and the acid producing capability can still be higher than 2g / L when the loaded liquid volume is 20% in case of liquid culture.

Description

technical field [0001] The invention relates to the field of wine-making microorganisms and wine-making technology, in particular to a glucose-inhibiting oxygen-tolerant caproic acid bacterium. Background technique [0002] The fermentation of Luzhou-flavor Daqu liquor adopts a unique mud cellar fermentation process, which belongs to the co-fermentation of various microbial groups and is a complex biochemical reaction system. The study found that ethyl caproate is the main aroma substance in Luzhou-flavor liquor, and the content of ethyl caproate directly affects the quality of Daqu liquor. It comes from the combination of caproic acid produced by a class of Clostridium-caproic acid bacteria that inhabits the pit mud and ethanol produced by yeast in the fermented grains. During the fermentation process, the formation of caproic acid and ethyl caproate is mainly carried out on the interface between the pit mud and the fermented grains. Clostridia can synthesize ethyl caproat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/52C12P7/16C12P7/40C12R1/145
CPCC12P7/52C12P7/16C12P7/40Y02E50/10
Inventor 方尚玲董孝元郭志豪常煦晋湘宜陈茂彬李良李小强李俊薇
Owner HUBEI UNIV OF TECH
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