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Pseudomonas aeruginosa and application thereof

A technology of Pseudomonas aeruginosa and pathogenic bacteria, applied in the field of agricultural microorganisms, to achieve the effect of reducing the disease index and obvious control effect

Pending Publication Date: 2021-07-23
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The preservation unit is the General Microbiology Center of China Microbiological Culture Collection Management Committee

Method used

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  • Pseudomonas aeruginosa and application thereof
  • Pseudomonas aeruginosa and application thereof
  • Pseudomonas aeruginosa and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The isolation and screening of embodiment 1 Pseudomonas aeruginosa HZ15

[0034] 1. Separation and screening

[0035] The larvae of a certain Lepidoptera insect were collected by the Plant Protection College of Yunnan Agricultural University in the "Yunnan Provincial Coffee Coffea Seedling Breeding Base" in Nanping Town, Simao District, Pu'er City, Yunnan Province. Adopt the following method steps to obtain bacterial strain:

[0036] 1. Rinse the body surface of the larvae with 75% alcohol for 3 times, each time for 2 minutes, and then rinse with sterile water for 3 times, then dissect to obtain a complete digestive tract of the larvae.

[0037] 2. Sterilization: All experimental equipment such as petri dishes, centrifuge tubes, test tubes, and pipette tips and sterile water are sterilized by high pressure at 0.1MPa, 121°C, and sterilized for 30 minutes.

[0038] 3. Grinding: Cut the digestive tract of the obtained larva into pieces, put it into a sterile centrifuge t...

Embodiment 2

[0056] The screening of the fermentation culture condition of embodiment 2 Pseudomonas aeruginosa

[0057] Inoculate the Pseudomonas aeruginosa HZ15 bacterial liquid on the YSP medium, and cultivate it under the conditions of temperature 36°C, pH value 7, rotation speed 220r / min, liquid volume 40mL, inoculum volume 2.5%, time 48h, light 12h , to obtain Pseudomonas aeruginosa HZ15 fermentation broth, for the optimization of the following fermentation conditions.

[0058] 1. Optimal culture medium

[0059] The strains were cultured in five mediums: YSP, NYBD, NA, LB and CM.

[0060] Peptone yeast sucrose medium (YSP), including the following components: peptone 1.00g, yeast extract powder 0.50g, sucrose 2.00g, distilled water 100mL;

[0061] Beef extract yeast glucose medium (NYBD), including the following components: beef extract 0.80g, yeast extract powder 0.50g, glucose 1.00g, distilled water 100mL;

[0062] Nutrient agar medium (NA), including the following components: be...

Embodiment 3

[0095] The inhibitory effect of embodiment 3 Pseudomonas aeruginosa HZ15 fermented liquid to Phytophthora tabacum pathogenic bacteria

[0096] Using the plate confrontation method, use a sterile puncher (5mm) to punch holes on the pathogen plate, insert the bacteria block into the center of the newly prepared OA plate, and then use a sterile inoculation loop to pick the antagonistic bacteria separately from the center of the OA plate Inoculate antagonistic bacteria 4 times at equal distances around 2.5 cm, set 3 replicates, measure the colony diameter on the treated plate after culturing at 28°C for 5 days, take the average value to calculate the bacteriostatic rate, and determine its inhibitory effect. The control group was not inoculated with antagonistic bacteria.

[0097]

[0098] like Figure 12 The results shown show that the colony diameter on the treated plate is about 2.01 cm, and the inhibition rate of Pseudomonas aeruginosa HZ15 on Phytophthora nicotianae is abo...

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Abstract

The invention discloses pseudomonas aeruginosa and application thereof, and belongs to the technical field of agricultural microorganisms. The preservation number of the pseudomonas aeruginosa is CGMCC No.21856, the preservation time is March 9, 2021, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing. The pseudomonas aeruginosa is separated from digestive tracts of lepidoptera insect larvae, and fermentation liquor of the pseudomonas aeruginosa has a good inhibition effect on phytophthora nicotianae, so that the biological agent for preventing and controlling phytophthora nicotianae is prepared by taking living bodies or metabolites of the pseudomonas aeruginosa as main components, the effect is good, the cost is low, and the pseudomonas aeruginosa has important significance on prevention and control of phytophthora nicotianae.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, and relates to a pseudomonas aeruginosa and an application thereof. Background technique [0002] Tobacco is an economic plant. In the main tobacco growing areas in my country, due to the long-term unreasonable planting methods and the application of chemical agents, the diseases on tobacco are becoming more and more serious, seriously affecting the quality of tobacco leaves and causing serious economic losses. , its prevention and control is facing enormous challenges. Tobacco black shank is caused by the pathogen Phytophthora nicotiana. It is a very common disease in the tobacco planting process. It occurs in all major tobacco planting areas in my country. In some areas, highly resistant diseased plants even appeared, which has become a threat to tobacco production. At present, the effective control measures for this kind of disease are mainly chemical control. When chemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/27A01P3/00C12R1/385
CPCA01N63/27C12N1/20
Inventor 吴国星曾舒泉何明川高熹钮徐融魏聪聪
Owner YUNNAN AGRICULTURAL UNIVERSITY
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