Carbonyl metabolite derivatization method and non-targeted metabonomics efficient analysis method

A derivatization and metabolite technology, applied in the field of metabolomics analysis, can solve the problem that the sensitivity needs to be improved, and achieve the effect of increasing detection sensitivity, eliminating noise interference and reducing instrument drift.
CN113156028APending Publication Date: 2021-07-23厦门市迈理奥科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
厦门市迈理奥科技有限公司
Publication Date
2021-07-23

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Abstract

The invention relates to a carbonyl metabolite derivatization method and a non-targeted metabonomics efficient analysis method.The carbonyl metabolite derivatization method comprises the steps of: 1, performing carbonyl metabolite extraction on a sample to be detected through a first solvent, then performing drying to remove the first solvent, and obtaining a dried sample; and 2, dissolving the dried sample, adding a pH adjusting reagent to adjust the pH value, adding dansylhydrazide, uniformly mixing, heating, incubating, cooling to terminate the reaction, drying to obtain a solid, and redissolving the solid to obtain a derivatized sample. According to the non-targeted metabonomics efficient analysis method, 12C labeling and 13C labeling are synchronously carried out by adopting the derivatization method, so that the metabolite detection sensitivity is improved, more metabolite can be detected at the same time, and the metabolite coverage rate is higher; and the detected metabolite is a peak pair, the influence caused by instrument drift and matrix is reduced, noise interference is eliminated, and quantification is more accurate.
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Description

technical field

[0001] The invention relates to a metabolomics analysis technology, in particular to a method for derivatization of carbonyl metabolites and a non-targeted metabolomics high-efficiency analysis method. Background technique

[0002] As an important part of systems biology, metabolomics has broad application prospects in the field of clinical medicine. Metabolomics analysis techniques include: NMR, GC-MS, CE-MS, and LC-MS. Although these techniques can detect metabolites to a certain extent, there are still many difficulties, such as low ionization efficiency of metabolites and mass spectrometry. Weak signal, lack of isotope internal standard for quantification, small number of detected metabolites, and many interferences.

[0003] For example, LC-MS uses liquid chromatography-mass spectrometry for analysis, and compares the respective metabolites in different samples to determine all the metabolites therein. Essentially, metabolic fingerprinting involves com...

Claims

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