Preparation method and application of vinasse alcohol-soluble peptide

A distiller's grains and alcohol-soluble technology, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problem that peptide powder cannot be added directly

Active Publication Date: 2021-07-30
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The authorized patent number is CN 107047921 B, which provides a method for preparing protein powder and polypeptide powder by using various distiller's grains, and the polypeptide powder obtained by this method cannot be directly added to liquor

Method used

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  • Preparation method and application of vinasse alcohol-soluble peptide
  • Preparation method and application of vinasse alcohol-soluble peptide

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Take 1000g of Luzhou-flavored distiller's grains fermented for 1 year, dilute with 3 times the mass of water after crushing, and the crude protein concentration in the system reaches 5.2g / 1000ml. Adding 4g NaOH to the mixed system to adjust the pH is 7.0, and then adding pretreatment combined enzymes (1g cellulase+1g amylase at the same time, wherein the enzyme activity of amylase is 4000U / g, and the enzyme activity of cellulase is 10000U / g, the same below) and hydrolysis combination enzyme (1g papain+1g neutral protease, wherein, the enzyme activity of papain is 60000U / g, the enzyme activity of neutral protease is 50000U / g, the same below), stirred at 50 ℃ Enzymatic hydrolysis for 3 hours. After enzymatic hydrolysis, add 3 times the volume of 45% ethanol to stir for 15 minutes, then alcohol precipitation for 12 hours, centrifuge at 4000rpm for 30 minutes, take the supernatant, discard the residue, and the content of alcohol-soluble crude peptide in the supernatant aft...

Embodiment 2

[0036] Take 1000g of Luzhou-flavor distiller's grains fermented for 1 year, dilute with 3 times the quality of water after crushing, and the crude protein concentration in the system reaches 5.2g / 1000ml. Add 4g NaOH to the mixed system to adjust the pH to 7.0, add pretreatment combination enzyme (1g amylase+1g cellulase) and hydrolysis combination enzyme (1g papain+1g trypsin, wherein the enzyme activity of trypsin is 10000U / g, the same below), stirred at 50°C for 3 hours. After enzymatic hydrolysis, add 3 times the volume of 60% ethanol and stir for 15 minutes, then alcohol precipitation for 12 hours, centrifuge at 4000rpm for 30 minutes, take the supernatant, discard the residue, and the content of alcohol-soluble crude peptide in the supernatant after two extractions is 1.9g / 1000ml. Add 2% activated carbon and 1% diatomaceous earth to the supernatant successively to filter and concentrate under reduced pressure until the volume is 500ml. Take 10g macroporous resin DA201 ...

Embodiment 3

[0038]Take 1000g of Fen-flavor distiller's grains that have been fermented for 1 year, grind them and dilute with 3 times the quality of water, and the concentration of crude protein in the system will reach 2.1g / 1000ml. Add 3.6g NaOH to the mixed system to adjust the pH to 7.0, add pretreatment combination enzyme (1g amylase + 1g cellulase) and hydrolysis combination enzyme (1g neutral protease + 1g trypsin), stir at 50°C for enzymolysis 3 Hour. After the enzymatic hydrolysis, add 3 times the volume of 45% ethanol to stir for 15 minutes, then alcohol precipitation for 12 hours, centrifuge at 4000rpm for 30 minutes, take the supernatant, discard the residue, and the alcohol-soluble crude peptide content in the supernatant after two extractions is 1.06g / 1000ml. Add 2% activated carbon and 1% diatomaceous earth to the supernatant successively to filter and then concentrate under reduced pressure until the volume is 500ml. Take 10g of macroporous resin AB-8 and DA201 and mix (1...

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Abstract

The invention discloses a preparation method and application of vinasse alcohol-soluble peptide, and belongs to the technical field of white spirit production. The method comprises the following steps: crushing vinasse, performing pretreatment by combining cellulose and amylase, performing hydrolysis by combining papain with polypeptide hydrolase or neutral protease, and after two-step hydrolysis, centrifuging and concentrating vinasse enzymatic hydrolysate to obtain vinasse crude peptide; and carrying out precipitation extraction, centrifugation, concentration, resin purification and nanofiltration interception grading on the vinasse crude peptide by using alcohol with a specific concentration to obtain a vinasse target alcohol-soluble polypeptide solution, concentrating and recovering ethanol, and drying to obtain the target product. The purity of the alcohol-soluble polypeptide prepared by the method is higher than 80%, the alcohol-soluble polypeptide is good in solubility, stable and free of precipitation after being added into a white spirit system, the molecular weight is smaller than 3000Da, and the alcohol-soluble polypeptide has potential biological activity and endows the product with dual characteristics of flavor and function.

Description

technical field [0001] The invention relates to a preparation method and application of distiller's alcohol soluble peptide, belonging to the technical field of liquor production and processing. Background technique [0002] Distiller's grains are the wastes after repeated saccharification and fermentation of grain raw materials such as sorghum and corn in the brewing industry. Limited by the existing fermentation agent and technology, a certain proportion of grain protein, cellulose, starch, etc. remain in distiller's grains. For a long time, due to the lag in development and research, the added value is low, and it has been largely discarded. In recent years, it has been found that distiller's grains contain functional nutrients such as polypeptides, conjugated phenols, and ferulic acid, and distiller's grains have become a potential functional food resource. Among them, some distiller's grain polypeptides may also migrate into liquor during the distillation process, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30C07K1/16
CPCC12P21/06C07K1/36C07K1/34C07K1/30C07K1/16Y02P60/87
Inventor 毛健周志磊刘小刚常瑞姬中伟刘双平韩笑
Owner JIANGNAN UNIV
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