Protease refining and drying method

Abstract

The invention discloses a protease refining and drying method comprising the steps: firstly extracting a crude protease raw material by using a sodium salt solution to improve the solubility of protease, adding a flocculating agent to flocculate and precipitate impurities in the protease solution, and removing the flocculated and precipitated impurities to obtain a relatively pure protease extracting solution; then further filtering and purifying the protease extracting solution by adopting a bag filtration method and a microfiltration method in sequence, then concentrating and desalting by utilizing ultrafiltration to obtain a pure protease concentrated solution; and finally, carrying out vacuum freeze drying on the protease concentrated solution by adopting a vacuum freeze drying machine. The protease refining method is simple and high in efficiency, and the obtained protease is high in purity, high in activity, stable and easy for industrial production. The protease concentrated solution is dried by adopting the vacuum freeze drying method and the vacuum freeze dryer, the drying speed is high, the energy consumption is low, the drying rate is high, and the obtained protease product is high in enzyme activity, good in enzyme activity stability and good in sanitary quality.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for refining and drying protease. Background technique [0002] Protease is a general term for a class of enzymes that hydrolyze protein peptide bonds, and can effectively decompose macromolecular proteins into polypeptides and free amino acids. The characteristic of protease catalyzing the hydrolysis of peptide bonds makes it one of the commonly used industrial enzymes. It is mainly used in food, leather, daily chemical, feed and pharmaceutical industries. It is used in meat processing, washing, leather manufacturing, and improving animal nutrition. , pharmaceuticals and other fields are playing an important role. At present, the production of protease is mostly crude extraction, with low enzyme activity, poor enzyme activity stability, and poor hygienic quality, and the obtained protease product level is low. The refining method of the protease, while the method is suita...

AI Technical Summary

Problems solved by technology

Because the vacuum freeze-drying method can well protect the activity of the enzyme, and the drying method is simple, the vacuum freeze-drying method is the most commonly used drying method for liquid enzyme preparations, but the existing vacuum freeze-drying machine is cumbersome due to the tedious work of feeding and discharging materials, and the separation Problems such as uneven temperature of the shelves, poor thermal conductivity of the tray, and the susceptibility of freeze-dried materials to microbial contamination can easily affect the efficiency, cost and product quality of vacuum freeze-drying of materials

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