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Lactobacillus gasseri LGV03 and application thereof

A technology of Lactobacillus gasseri and bacterial suspension, applied in the field of microorganisms, can solve problems such as affecting the promotion of probiotics and their products, lack of scientific research evidence for probiotic functions, and dependence on imports of probiotic strains, and achieve good prevention of allergic reactions. , inhibition of mast cell degranulation

Active Publication Date: 2021-08-06
南方医科大学第七附属医院佛山市南海区第三人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The probiotic strains used by domestic production enterprises have long relied on imports, and foreign strains may not be suitable for the physiological conditions of the gastrointestinal tract of Chinese residents
In addition, the function of probiotics lacks strong scientific research evidence, which seriously affects the promotion of probiotics and their products

Method used

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  • Lactobacillus gasseri LGV03 and application thereof
  • Lactobacillus gasseri LGV03 and application thereof
  • Lactobacillus gasseri LGV03 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and identification of Lactobacillus gasseri LGV03

[0029] (1) Separation: After serially diluting the secretions of the healthy female reproductive tract, they were respectively inoculated in TPY solid medium, MRS solid medium, BDS solid medium, and BS solid medium, cultured anaerobically at 37°C for 48 hours, and picked Single colonies on the plate were streaked to obtain pure colonies. The pure colony on the plate was inoculated into MRS liquid medium, cultured anaerobically at 37°C for 12-16h, added 20% glycerol, and stored in a -80°C refrigerator.

[0030] (2) Molecular biological identification of the strain: Genomic DNA was extracted from the obtained strain, and the full-length 16S rDNA fragment was amplified by PCR using 16S rDNA universal primers 27F and 1492R, followed by sequencing to identify the species of the strain. The sequences of the universal primers 27F and 1492R are as follows:

[0031] 27F: 5'-AGAGTTTGATCCTGGCTCAG-3'; SEQ ID ...

Embodiment 2

[0049] Preparation of embodiment 2 Lactobacillus gasseri LGV03 fermentation supernatant (extracellular secretion), bacterial suspension (thalline)

[0050] Lactobacillus gasseri LGV03 was activated and cultured and inoculated in MRS liquid medium. After culturing at 37°C for 15 hours, the concentration of fermentation bacteria was adjusted to 2×10 8 CFU / mL, centrifuged at 4°C, 8000r / min for 10min to obtain the culture supernatant and cell pellet, the supernatant was filtered through a 0.22μm filter membrane to obtain the fermentation supernatant (extracellular secretion); the cell pellet was filtered through PBS twice After the first wash, the cells were resuspended with PBS, and the cell concentration was adjusted to 2×10 8 CFU / mL to obtain bacterial suspension (bacteria).

Embodiment 3

[0051] Example 3 Effect of Lactobacillus gasseri LGV03 on Compound 48 / 80 stimulating zebrafish to secrete Tryptase

[0052] Sodium cromoglycate and Na-benzoyl-DL-arginine-p-nitroamide hydrochloride (BAPNA) were purchased from Shanghai Yuanye Biotechnology Co., Ltd., and Compound 48 / 80 was purchased from Sigma-Aldrich.

[0053] The healthy wild-type AB line zebrafish developed to 5dpf (days post fertilization) was selected and placed in a 96-well cell culture plate, 10 / well. The experiment set up normal group, model group, intervention group (positive control group, bacterial suspension group, fermentation supernatant group) and solvent zeroing group, and each group set up 6 replicate wells. The normal group was added with PBS, the model group was added with PBS, the positive control group was added with cromolyn sodium solution (100 μg / mL), the bacterial suspension group was added with bacterial suspension, the fermentation supernatant group was added with fermentation superna...

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Abstract

The invention discloses lactobacillus gasseri LGV03 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus gasseri LGV03 is CGMCC No.22010. A fermentation supernatant and a bacterial suspension of the lactobacillus gasseri LGV03 can remarkably inhibit the secretion of Trypase by the zebra fish stimulated by Compound 48 / 80 in vivo, can inhibit in-vivo mast cell degranulation, and have the potential of being applied to in-vivo anaphylactic reaction prevention. The lactobacillus gasseri LGV03 disclosed by the invention has a huge potential application prospect in the aspect of preventing anaphylactic reaction.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of Lactobacillus gasseri LGV03 and its application. Background technique [0002] Allergic diseases are gradually becoming a global public health problem, whether in developed or developing countries, various allergic diseases include asthma, allergic rhinitis and conjunctivitis, drug allergy, food allergy, insect bite allergy, skin eczema (atopic dermatitis), urticaria or severe systemic allergic reactions (anaphylaxis) all affect people's health and daily life to varying degrees, and some serious cases can even be life-threatening. Therefore, allergic diseases are listed as one of the three major diseases that should be prevented and treated in the 21st century. According to worldwide epidemiological surveys, about 22% of the population suffers from different kinds of allergic diseases. [0003] Allergies are hypersensitivity reactions to harmless environmental anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P37/08C12R1/225
CPCA23L33/135A61K35/747A61P37/08A23V2002/00A23V2400/145A23V2200/304Y02A50/30
Inventor 邱峰
Owner 南方医科大学第七附属医院佛山市南海区第三人民医院
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