Check patentability & draft patents in minutes with Patsnap Eureka AI!

Microbial reinforcement method for fractured rock mass

A cracked rock mass and microbial technology, applied in the field of cracked rock mass reinforcement, can solve problems such as unsatisfactory bonding effect, achieve the effect of enhancing the bonding effect, promoting absorption, and improving calcification

Pending Publication Date: 2021-08-06
HUANENG LANCANG RIVER HYDROPOWER
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the microbial reinforcement of fractured rock mass has a good application prospect, the bonding effect of the current microbial reinforcement method on the fractured rock mass is still not ideal.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial reinforcement method for fractured rock mass
  • Microbial reinforcement method for fractured rock mass
  • Microbial reinforcement method for fractured rock mass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Consolidation nutrient solution composition is in the present embodiment:

[0047] Tryptone 9g / L, soybean peptone 2g / L, anhydrous calcium chloride 50g / L, urea 34g / L, methyl-α-D-glucopyranoside 8g / L, tea saponin 0.5g / L, remaining The amount is water.

[0048] Microbial reinforcement of fractured rock mass:

[0049] (1) Soak the fractured rock sample with distilled water for 24 hours to remove impurities on the surface of the sample;

[0050] (2) Align the two petals of the fissure rock sample and push them into a sleeve with an inner diameter of 50mm. The bottom of the sample is covered with permeable stones, which are flush with the bottom of the sleeve, and a dropper is set on the top of the sleeve;

[0051] (3) Add 15mL of concentrated Bacillus pasteurianus solution (the concentration of bacteria solution is 2.6×10 9 cells / ml);

[0052] (4) After the dropwise addition of the Bacillus pasteurei concentrated solution is completed, add 150mL of DSMZ nutrient solution...

Embodiment 2

[0056] Consolidation nutrient solution composition is in the present embodiment:

[0057] Tryptone 11g / L, soybean peptone 3g / L, anhydrous calcium chloride 48g / L, urea 35g / L, methyl-α-D-glucopyranoside 9g / L, tea saponin 0.4g / L, remaining The amount is water.

[0058] Microbial reinforcement of fractured rock mass:

[0059] (1) Soak the fractured rock sample with distilled water for 24 hours to remove impurities on the surface of the sample;

[0060] (2) Align the two petals of the fissure rock sample and push them into a sleeve with an inner diameter of 50mm. The bottom of the sample is covered with permeable stones, which are flush with the bottom of the sleeve, and a dropper is set on the top of the sleeve;

[0061] (3) Add 15mL of Bacillus pasteurianum concentrate (the concentration of the bacteria solution is 2.0×10 9 cells / ml);

[0062] (4) After the dropwise addition of the Bacillus pasteurei concentrated solution is completed, add 120mL of DSMZ nutrient solution into...

Embodiment 3

[0065] Consolidation nutrient solution composition is in the present embodiment:

[0066] Tryptone 10g / L, soybean peptone 2g / L, anhydrous calcium chloride 49g / L, urea 33g / L, methyl-α-D-glucopyranoside 10g / L, tea saponin 0.5g / L, remaining The amount is water.

[0067] Microbial reinforcement of fractured rock mass:

[0068] (1) Soak the fractured rock sample with distilled water for 24 hours to remove impurities on the surface of the sample;

[0069] (2) Align the two petals of the fissure rock sample and push them into a sleeve with an inner diameter of 50mm. The bottom of the sample is covered with permeable stones, which are flush with the bottom of the sleeve, and a dropper is set on the top of the sleeve;

[0070] (3) Add 15mL of Bacillus subtilis concentrated solution (the concentration of bacteria solution is 2.4×10 9 cells / ml);

[0071] (4) After the dropwise addition of the Bacillus pasteurei concentrated solution is completed, add 140mL of DSMZ nutrient solution i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a microbial reinforcement method for fractured rock mass, and belongs to the technical field of fractured rock mass reinforcement. The method comprises the following steps that firstly, a microorganism concentrated solution is injected into a fractured rock mass, and then a DSMZ nutrient solution and a consolidation nutrient solution are sequentially added, wherein the used microbial strain is bacillus pasteurii, or bacillus alcaligenes, bacillus licheniformis or bacillus subtilis, and the consolidation nutrient solution comprises the following components of tryptone, soy peptone, anhydrous calcium chloride, urea, methyl-alpha-D-glucopyranoside, tea saponin and water. According to the method, through the consolidation nutrient solution, the calcium ion absorption effect of bacillus can be enhanced, the calcification effect is improved, and therefore the excellent fractured rock mass consolidation effect is achieved.

Description

technical field [0001] The invention relates to the technical field of fissure rock mass reinforcement, in particular to a microbial reinforcement method for fissure rock mass. Background technique [0002] In the plateau region, fractured rock mass develops in valley slopes, and the depth of development increases with elevation. Under natural conditions and construction interference, local rock blocks in cracked rock mass may collapse at any time. On high and steep mountains, falling rocks pose a great threat to the safety of engineering construction roads, personnel and equipment. This danger is always and are common in the mountains on both sides of the strait, therefore, it is necessary to strengthen and treat the fractured rock mass. [0003] When using traditional methods to strengthen and treat the fractured and fractured rock mass all over the mountains on both sides of the river, the amount of work is huge, the construction period is extremely long, the cost is una...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): E21D9/00C12N1/20C12N1/38E02D17/20C12R1/07C12R1/10C12R1/125
CPCE21D9/002C12N1/20C12N1/38E02D17/20E02D3/10
Inventor 陈鸿杰
Owner HUANENG LANCANG RIVER HYDROPOWER
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com