Filler comprising hyaluronic acid hydrogel having excellent filling properties
A technology of hyaluronic acid and filling performance, which is applied in the direction of organic active ingredients, skin care preparations, medical preparations containing active ingredients, etc., and can solve problems such as selection difficulties
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Embodiment 1
[0068] Example 1: Preparation of filler containing hyaluronic acid hydrogel
[0069] In order to prepare the hyaluronic acid hydrogel-containing filler of the present invention, the following procedures are performed.
[0070] In detail, sodium hyaluronate having an average molecular weight of 2.5 to 3.5 MDa, sodium hydroxide, and 1,4-butanediol diglycidyl ether (BDDE) as a crosslinking agent were weighed. During the reaction, the concentration of sodium hyaluronate was 16% by weight and the mole % of BDDE relative to the added sodium hyaluronate units was 4%. Separately, an aqueous sodium hydroxide (NaOH) solution having a concentration of 0.25N was prepared and filtered. Put the weighed sodium hyaluronate, 0.25N sodium hydroxide aqueous solution and 1,4-butanediol diglycidyl ether (BDDE) into the mixer tank and mix evenly, put the mixer tank into a constant temperature water bath and react overnight at 30°C to complete the crosslinking reaction. Afterwards, the reacted ...
experiment example 1
[0075] Experimental Example 1: MSD measurement of tracer particles in a filler containing hyaluronic acid hydrogel using microrheology quantity
[0076] In order to analyze the performance of the prepared Example 1, the commercially available hyaluronic acid hydrogel-containing fillers of Comparative Examples 1 to 5 were used together with Example 1 in the experiment, as shown in Table 1.
[0077] In detail, the Brownian motion of the tracer particles dispersed in the sample was photographed using a video microscope, and then the motion of the tracer particles was analyzed using an image processing software (Matlab) ( figure 2 ).
[0078]Specifically, a test sample was prepared by dispersing 1 volume % of tracer particles (polystyrene particles having a diameter of 1 μm) in 1 mL of the filler of Example 1. Microrheological experiments are performed by loading a filler sample in which tracer particles are dispersed between transparent glass slides, and recording the traje...
experiment example 2
[0092] Experimental example 2: Confirmation of wrinkle-improving performance of fillers containing hyaluronic acid hydrogel
[0093] In order to confirm whether the WIE parameter of Experimental Example 1 is substantially related to the wrinkle-improving performance, the wrinkle-improving performance of the hyaluronic acid fillers of Example 1 and Comparative Examples 1 to 5 shown in Table 1 were measured according to the following method.
[0094] In order to measure the lifting ability (which is a parameter showing wrinkle-improving performance), the elasticity and cohesion of the filler were measured by a rheometer (ARES-G2, TA Instruments Inc.), and these two values were multiplied to calculate improving capability. The results confirming such elasticity, cohesion and lifting ability are shown in the following Table 2 and Figure 4 middle.
[0095] Specifically, elasticity is measured by determining how much stress, expressed in Pa, is required to load a filler betwe...
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