Lactobacillus johnsonii capable of relieving diarrhea caused by ETEC and application of lactobacillus johnsonii
A technology of Lactobacillus johnsonii and Lactobacillus johnsonii, applied in the field of microbiology, can solve the problems of uncertainty about the quality of the effect, and achieve the effects of increasing body weight, reducing feces water content, and reducing the level of immune factors
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Embodiment 1
[0067] Example 1: Screening and strain identification of Lactobacillus johnsonii CCFM1181
[0068] 1. Screening: Take the duck feces from Huadian, Jilin as a sample, take 0.5g of the sample preserved in 30% (v / v) glycerol and add it in a 10mL centrifuge tube containing 4.5mL of normal saline under a sterile environment to obtain 10 -1 Diluent, repeat the above dilution steps to obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Dilution: draw 100 μL of gradient dilutions of different gradients and spread them on the MRS solid medium, incubate at 37°C for 72 hours to obtain a diluted coating plate; pick typical colonies on the diluted coating plate and inoculate them into the MRS liquid medium respectively , Cultivate at 37°C for 48h to obtain the bacterial liquid.
[0069] 2. Identification: After numbering the strains corresponding to the isolated and purified bacterial liquids, perform strain identification, Gram staining, physiological and biochemical experiments with reference...
Embodiment 2
[0080] Example 2: Effects of Lactobacillus johnsonii CCFM1181 on body weight and feces water content of mice with diarrhea
[0081] Specific steps are as follows:
[0082] 40 BALB / c mice were taken and fed for 1 week under the conditions of room temperature 22-24°C, humidity 40%-60%, 12h / 12h day and night, free access to food and water, and were randomly divided into 5 groups. There are 8 rats in each group, and the 5 groups are: control group, model group, Brady's yeast group (CNCMI-745 group) fed with Brady's yeast liquid, and Lactobacillus johnsonii (Lactobacillus johnsonii) FAHBZ6L5 bacterial liquid The FAHBZ6L5 group, and the CCFM1181 group fed Lactobacillus johnsonii (Lactobacillus johnsonii) CCFM1181 bacterial solution.
[0083] The experiment lasted for 3 weeks: the experiment started after 1 week of adaptive feeding of the animals. Beginning 7 days before modeling, and continuing until the end of the experiment (excluding those 3 days of antibiotic treatment), mice ...
Embodiment 3
[0088] Example 3: Effect of Lactobacillus johnsonii CCFM1181 on the pathological damage of the jejunum of mice with diarrhea
[0089] The construction method of the animal model is the same as in Example 2. After the experiment, the mice were sacrificed, and the jejunum of the mice was taken, soaked in 4% (v / v) paraformaldehyde for 24 hours to obtain a fixed jejunum tissue; the fixed jejunum After the tissue is dehydrated, transparent, and wax-immersed in sequence, the tissue is embedded in a wax block with a Lycra paraffin embedding machine to obtain a wax block that embeds the jejunum tissue; wherein, the specific steps of dehydration, transparency, and wax immersion are as follows: ( 1) Dehydration: Dehydrate the fixed tissue through 70%, 80% and 90% (v / v) gradient ethanol solutions in sequence, each gradient is 30min, and then put in 95% and 100% (v / v) Alcohol solution twice, 20 minutes each time; (2) Transparency: Put the tissue in a mixture of alcohol and xylene in an eq...
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