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Lactobacillus johnsonii capable of relieving diarrhea caused by ETEC and application of lactobacillus johnsonii

A technology of Lactobacillus johnsonii and Lactobacillus johnsonii, applied in the field of microbiology, can solve the problems of uncertainty about the quality of the effect, and achieve the effects of increasing body weight, reducing feces water content, and reducing the level of immune factors

Active Publication Date: 2021-08-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, studies have shown that a small number of probiotics can indeed prevent and / or treat some special diseases. Although it has been reported in the literature that Lactobacillus johnsonii alleviates the diarrhea caused by ETEC, the quality of the effect is still uncertain

Method used

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  • Lactobacillus johnsonii capable of relieving diarrhea caused by ETEC and application of lactobacillus johnsonii
  • Lactobacillus johnsonii capable of relieving diarrhea caused by ETEC and application of lactobacillus johnsonii
  • Lactobacillus johnsonii capable of relieving diarrhea caused by ETEC and application of lactobacillus johnsonii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1: Screening and strain identification of Lactobacillus johnsonii CCFM1181

[0068] 1. Screening: Take the duck feces from Huadian, Jilin as a sample, take 0.5g of the sample preserved in 30% (v / v) glycerol and add it in a 10mL centrifuge tube containing 4.5mL of normal saline under a sterile environment to obtain 10 -1 Diluent, repeat the above dilution steps to obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Dilution: draw 100 μL of gradient dilutions of different gradients and spread them on the MRS solid medium, incubate at 37°C for 72 hours to obtain a diluted coating plate; pick typical colonies on the diluted coating plate and inoculate them into the MRS liquid medium respectively , Cultivate at 37°C for 48h to obtain the bacterial liquid.

[0069] 2. Identification: After numbering the strains corresponding to the isolated and purified bacterial liquids, perform strain identification, Gram staining, physiological and biochemical experiments with reference...

Embodiment 2

[0080] Example 2: Effects of Lactobacillus johnsonii CCFM1181 on body weight and feces water content of mice with diarrhea

[0081] Specific steps are as follows:

[0082] 40 BALB / c mice were taken and fed for 1 week under the conditions of room temperature 22-24°C, humidity 40%-60%, 12h / 12h day and night, free access to food and water, and were randomly divided into 5 groups. There are 8 rats in each group, and the 5 groups are: control group, model group, Brady's yeast group (CNCMI-745 group) fed with Brady's yeast liquid, and Lactobacillus johnsonii (Lactobacillus johnsonii) FAHBZ6L5 bacterial liquid The FAHBZ6L5 group, and the CCFM1181 group fed Lactobacillus johnsonii (Lactobacillus johnsonii) CCFM1181 bacterial solution.

[0083] The experiment lasted for 3 weeks: the experiment started after 1 week of adaptive feeding of the animals. Beginning 7 days before modeling, and continuing until the end of the experiment (excluding those 3 days of antibiotic treatment), mice ...

Embodiment 3

[0088] Example 3: Effect of Lactobacillus johnsonii CCFM1181 on the pathological damage of the jejunum of mice with diarrhea

[0089] The construction method of the animal model is the same as in Example 2. After the experiment, the mice were sacrificed, and the jejunum of the mice was taken, soaked in 4% (v / v) paraformaldehyde for 24 hours to obtain a fixed jejunum tissue; the fixed jejunum After the tissue is dehydrated, transparent, and wax-immersed in sequence, the tissue is embedded in a wax block with a Lycra paraffin embedding machine to obtain a wax block that embeds the jejunum tissue; wherein, the specific steps of dehydration, transparency, and wax immersion are as follows: ( 1) Dehydration: Dehydrate the fixed tissue through 70%, 80% and 90% (v / v) gradient ethanol solutions in sequence, each gradient is 30min, and then put in 95% and 100% (v / v) Alcohol solution twice, 20 minutes each time; (2) Transparency: Put the tissue in a mixture of alcohol and xylene in an eq...

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Abstract

The invention discloses lactobacillus johnsonii capable of relieving diarrhea caused by ETEC and application of the lactobacillus johnsonii, and belongs to the technical field of microorganisms. According to the invention, a strain of lactobacillus johnsonii CCFM1181 is screened out, and the strain has the effect of relieving diarrhea, which is specifically reflected in that the water content of excrement of diarrhea mice is remarkably reduced, the pathological injury of jejunum of the diarrhea mice is remarkably relieved, the level of inflammatory factors in serum of the diarrhea mice is remarkably reduced, the content of enterotoxin ST in the serum of the diarrhea mice is remarkably reduced, and the content of acetic acid in the excrement of the diarrhea mice is obviously improved, and the lactobacillus johnsonii has a huge application prospect in preparation of medicines for preventing and / or treating diarrhea or functional foods and health-care products for assisting in relieving diarrhea symptoms.

Description

technical field [0001] The invention relates to a strain of Lactobacillus johnsonii capable of relieving diarrhea caused by ETEC and its application, belonging to the technical field of microorganisms. Background technique [0002] ETEC, enterotoxic Escherichia coli, settles on the surface of the small intestine, does not damage or invade the epithelial cells of the intestinal mucosa, and causes secretory diarrhea by producing enterotoxin. Helicobacter is one of the main pathogens of "tourist's diarrhea" in developed countries, a common cause of "adult cholera syndrome", and an important pathogen of children's diarrhea. Its incidence rate is second only to rotavirus. [0003] At the same time, ETEC is also an important pathogen that causes diarrhea in domestic animals, especially young animals (newborn piglets, calves, etc.). After newborn piglets are infected with ETEC, they often die due to severe watery diarrhea and rapid dehydration. The morbidity and mortality are high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/12A23C9/123A23L11/50A23L13/40A23L19/00A23L29/00A23L2/38A23L7/104A23L33/00C12R1/225
CPCC12N1/20A61K35/747A61P1/12A23C9/1234A23L13/45A23L19/00A23L29/065A23L2/382A23L7/104A23L33/00A23V2002/00A23V2400/151A23V2200/32Y02A50/30
Inventor 杨波陈卫岳月崔树茂王刚赵建新张灏
Owner JIANGNAN UNIV
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