Abdominal wall tissue repair patch and preparation method thereof

Abstract

The invention relates to an abdominal wall tissue repair patch and a preparation method thereof. The repair patch comprises a growth layer and an isolation layer, wherein the growth layer is a micro-nano fiber-hydrogel interpenetrating three-dimensional network composed of a micro-nano fiber three-dimensional network and hydrogel, and the isolation layer is a micro-nano fiber membrane; the average included angle between the axial direction of the micro-nano fibers in the growth layer and the isolation layer and the longitudinal direction of the repair patch is 5-30 degrees; the micro-nano fiber three-dimensional network is a self-locking three-dimensional network constructed by micro-nano fibers with high length-diameter ratio, and more than 99% of the fibers are in a monodisperse state; The method comprises the following steps: firstly, performing electrostatic spinning to prepare the isolation layer, and adding a hydrogel precursor to prepare a composite layer while receiving micro-nano fibers on the isolation layer; and expanding the hydrogel precursor to prepare the micro-nano fiber-hydrogel three-dimensional interpenetrating network abdominal wall tissue repair patch. The method is simple and controllable in process; and the patch has good mechanical strength, force conduction performance and tensile fatigue resistance, and cells can be induced to be aligned along the patch under force stimulation.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and relates to an abdominal wall tissue repair patch and a preparation method thereof, in particular to a micro-nano fiber-hydrogel three-dimensional interpenetrating network abdominal wall tissue repair patch and a preparation method thereof. Background technique [0002] Abdominal wall defect is a clinically common disease. When the abdominal wall defect is large, it is usually necessary to use implant materials to suture the abdominal wall defect to reduce the tension of the abdominal wall and speed up the wound repair of the patient. The implant materials currently used for the repair of abdominal wall defects can be divided into the following two types: synthetic mesh and biological mesh. [0003] Synthetic patches mainly include microporous membrane patches and mesh patches. Among them, the mesh patch is one of the common artificial patches due to its light weight and excellent...

AI Technical Summary

Problems solved by technology

However, the following problems still exist: 1. The mechanical compatibility is poor; 2. The degradation rate of decellularized tissue is often faster than the growth rate of human new abdominal wall tissue, which often leads to the failure of such patches

3. When the decellularized tissue is directly used for surgical repair, the material is too dense, and it is difficult for host cells and new tissues to grow into the material, which is not conducive to defect repair

Under the cutting action of sutures or fixation nails during implantation, the hydrogel is easy to tear off, resulting in failure

After implantation, the fatigue resistance of the hydrogel is also insufficient under the repeated stretching motion of the abdominal wall tissue

Moreover, as a scaffold material for abdominal wall tissue engineering, hydrogel itself has shortcomings in promoting tissue regeneration. Abdominal wall tissue has an oriented structure, while hydrogel is a disordered polymer network structure, which is not conducive to the oriented growth of cells, and eventually forms There are also structural differences between the tissue of the human body and the abdominal wall tissue of the human body