Hybridoma cell strain RSVN4C3 secreting anti-respiratory syncytial virus monoclonal antibody

Abstract

The invention discloses a hybridoma cell strain RSVN4C3 secreting an anti-respiratory syncytial virus monoclonal antibody. The hybridoma cell strain RSVN4C3 is preserved in China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: C2020254. The anti-respiratory syncytial virus nucleocapsid protein monoclonal antibody secreted by the hybridoma cell strain RSVN4C3 is used for preparing a kit for detecting or diagnosing respiratory syncytial virus infection, and the kit can be an immune colloidal gold test strip or an ELISA kit. The hybridoma cell strain RSVN4C3 can stably secrete the anti-respiratory syncytial virus monoclonal antibody (MAb), the reactivity and specificity of the monoclonal antibody (MAb) are identified, and a foundation is laid for future diagnosis of RSV virus infection and research of an infection mechanism.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell strain RSVN4C3 secreting monoclonal antibody against respiratory syncytial virus. Background technique

[0002] Human respiratory syncytial virus (RSV) is the leading cause of acute lower respiratory infection (ALRI) worldwide, leading to hospitalization in children younger than 5 years and immunocompromised adults. RSV infection is associated with high morbidity and mortality from acute lower respiratory infections (ALRIs) in children in both developed and developing countries. In developed countries, it is the leading cause of infant hospitalization within one month of birth; in developing countries, RSV is the leading cause of infant death, and about 34 million children under the age of 5 develop severe RSV disease each year, of which 350 million children required hospitalization and between 66,000 and 199,000 died, 99 percent of which occurred in developing coun...

Images