Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker related to excellent lactation character of Mediterranean buffalo and application

A technology for lactation traits and water buffalo, which is applied in the field of water buffalo molecular marker screening and assisted selection, and can solve the problems of low economic benefits of buffalo breeding

Active Publication Date: 2021-08-17
HUAZHONG AGRI UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are rich breeds of buffalo at home and abroad, but there is no specialized lactating breed / strain at present. Even the breeds such as Moura, Nile-Lafite, and Mediterranean buffalo with high average yield per unit area have an average milk yield of only Holstein cows. 1 / 5-1 / 8 of that, resulting in low economic benefits of buffalo farming (Jiang et al 2010)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker related to excellent lactation character of Mediterranean buffalo and application
  • Molecular marker related to excellent lactation character of Mediterranean buffalo and application
  • Molecular marker related to excellent lactation character of Mediterranean buffalo and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Identification of ApoB_SNP1 to ApoB_SNP6 markers associated with lactation traits of Mediterranean buffaloes and primer design for detecting genotypes at ApoB_SNP1 to ApoB_SNP6 loci

[0066] 1. Test object

[0067] The trial selected 350 Mediterranean buffalo whose DNA samples came from Italian pastures; all lactation records were provided by the Italian Breeding Association (AIA) and the Italian Agricultural Research Council (CAR).

[0068] 2. Primer Design

[0069] According to the buffalo ApoB gene sequence in GenBank (accession number: NC_037556.1) as a template, use Primer5.0 primer design software to design primer pairs as shown in the fragments of SEQ ID NO: 7-14, and entrust Beijing Qingke Biotechnology Co., Ltd. Co., Ltd. (Wuhan) synthesis.

[0070] 3. PCR amplification reaction

[0071] Using the Mediterranean buffalo genomic DNA as a template, the PCR amplification reaction was carried out according to the following procedures: the PCR reaction ...

Embodiment 2

[0080] Example 2: Application of markers ApoB_SNP1 to ApoB_SNP6 in association analysis of lactation traits in Mediterranean buffaloes

[0081] In order to determine whether the detected ApoB_SNP1 to ApoB_SNP6 markers are related to the differences in lactation phenotypes of Mediterranean buffaloes, Mediterranean buffaloes (350 heads) were selected as test materials in this example. The polymorphism was detected by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and the correlation between different genotypes and lactation traits of buffalo was analyzed. The general linear model in SAS9.4 software was used for the association analysis between genotype and phenotype values. The SNPs were the same as the haplotype association analysis model, and the analysis model was: Y ijkl =u+G i +P j +S k +F l +PS jk +PF jl +SF kl +e. Among them, Y ijkl is the observed value of the trait; u is the total mean value of the trait; G i is th...

Embodiment 3

[0091] Example 3: Identification of haplotype combinations of ApoB_SNP1 to ApoB_SNP6 loci with excellent lactation traits

[0092] (1) Construction of haplotype and haplotype combination

[0093] Haploview software was used to analyze the haplotypes of ApoB_SNP1 to ApoB_SNP6, and at the same time, the genotype data of all individual ApoB_SNP1 to ApoB_SNP6 sites were input into the PHASE program to calculate the genotype of each individual, and at the same time calculate the composition between the sites. The degree of linkage disequilibrium.

[0094] Haplotype block analysis is attached Figure 7 shown. It was found that SNP sites g.-1718A>G, g.-1823G>C, g.-2063A>C participated in forming a haplotype block 1 (haplotype block), SNP sites g.37375G>A and g.40787A >G participates in a haplotype block 2, both of which are in linkage disequilibrium.

[0095] (2) Association analysis of different haplotype combinations and milk production traits

[0096] Calculate the haplotype ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a molecular marker related to the excellent lactation character of a Mediterranean buffalo and application. The invention provides an SNP marker which is cloned from an ApoB gene, is used independently or in combination and is related to the lactation character of the buffalo, the SNP marker is selected from SNP1-SNP6, and the nucleotide sequence of the SNP marker is shown as SEQ ID NO: 1-6. The AA genotype of the SNP1, the GG genotype of the SNP2, the AA genotype of the SNP3, the GG genotype of the SNP4, the GG genotype of the SNP5 and the GG genotype of the SNP6 are dominant genotypes. The invention also provides two dominant haplotype combinations, namely a block 1 and a block 2. The SNP marker provided by the invention is closely associated with the lactation character of the Mediterranean buffalo, the lactation character of a detected object can be rapidly determined by detecting the molecular marker, the purpose of selecting the target character is achieved, and the SNP marker has the advantages of being rapid, accurate and free of interference of environmental conditions.

Description

technical field [0001] The invention belongs to the technical field of buffalo molecular marker screening and auxiliary selection. It specifically involves the SNP markers and their haplotype combinations and applications related to the excellent lactation traits of Mediterranean buffaloes. Background technique [0002] Buffaloes are the second largest dairy producing livestock in the world, accounting for 12% of total global milk production (Zicarelli 2020). Buffalo milk is rich in nutritional value, and its total protein, medium-chain fatty acid, conjugated linoleic acid, retinol and vitamin E content are all higher than those of Holstein milk. It is a high-quality raw material for processing and producing a variety of high-end dairy products, such as cheese, Yogurt and cream etc. (Mahmood and Sumaira 2010). Therefore, in the past two decades, the buffalo industry has received more and more national attention, and the buffalo herd has grown at a rate of 2% per year (Ahma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124C12Q2600/172
Inventor 滑国华曹家瑄林雨昕孙晖杨利国李华钊吴祺鹏
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com