Preparation method of serum carbon dioxide determination reagent ball and reagent ball

Abstract

The embodiment of the invention relates to the technical field of medical immune in-vitro diagnosis, and particularly relates to a preparation method of a serum carbon dioxide determination reagent ball and the reagent ball. The method comprises the following steps of: dripping a mixed solution into liquid nitrogen in a liquid drop form, so that liquid drops form an ice ball; and then freezing and drying the ice ball to prepare the serum carbon dioxide determination reagent ball, wherein the mixed solution is prepared from the following components: 5 to 150mmol / L of a buffering solution, 5 to 20g / L of phosphoenolpyruvic acid, 0.1 to 10g / L of reduced coenzyme I, 5 to 20KU / L of malic dehydrogenase, 0.1 to 5KU / L of phosphoenolpyruvate carboxylase, 0.1 to 10g / L of a stabilizer and 10 to 100g / L of an excipient. The excipient with the dosage range can ensure the shape and re-melting solubility of the reagent ball and the activity of reactants is protected; the excipient is good for completely freezing and drying the reagent ball. Namely, the serum carbon dioxide determination reagent ball prepared by the method has relatively good shape and re-melting solubility and can be completely frozen and dried, so that the serum carbon dioxide determination reagent ball has relatively high stability and precision and can realize in-time diagnosis.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of in vitro medical immunodiagnosis, in particular to a method for preparing a serum carbon dioxide measurement reagent ball and the reagent ball. Background technique [0002] 90% to 95% CO in serum or plasma 2 to HCO 3 - It is the main buffer base for the body to maintain acid-base balance, and it is also one of the commonly used indicators for judging acid-base metabolism. blood CO 2 Content determination provides an important basis for judging the progress and curative effect of acid-base balance disorders, and is widely used in clinical practice and is one of the indispensable emergency items. [0003] At present, the enzymatic method used clinically to measure serum carbon dioxide, on the one hand, the reagent solution needs to add a circulatory regeneration system of reducing coenzyme I (NADH), and the stability is poor. On the other hand, its dry powder reagents have po...

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Problems solved by technology

[0003] At present, the enzymatic method used clinically to measure serum carbon dioxide, on the one hand, the reagent solution needs to add a circulatory regeneration system of reducing coenzyme I (NADH), and the stability is poor

On the other hand, the stability of the dry powder reagent after reconstitution is poor, the validity period of the liquid reagent is short, and it relies on a large-scale automatic biochemical analyzer, which is difficult to apply to the patient's immediate diagnosis (point-of-care, POCT). certain limit

Although there are existing freeze-dried reagent balls used to detect other items, the shape of the existing freeze-dried reagent balls is poor, and it is difficult to completely freeze-dry, resulting in lower stability and accuracy.

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