Preparation method and application of phycomycete symbiont for degrading culture wastewater containing high-concentration antibiotics and having low C/N ratio

A technology for the symbiosis of aquaculture wastewater and algae and bacteria, which is applied in the field of water treatment, can solve the problems that the treatment process cannot be carried out simultaneously, the process is complicated, and the cost is high, and achieves the effects of good nitrogen and phosphorus removal effect, simple and easy operation, and low preparation cost.

Active Publication Date: 2021-08-24
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the technical problems of the existing activated sludge biological treatment process of aquaculture wastewater is complicated, and each treatment process cannot be carried out synchronously, resulting in

Method used

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  • Preparation method and application of phycomycete symbiont for degrading culture wastewater containing high-concentration antibiotics and having low C/N ratio
  • Preparation method and application of phycomycete symbiont for degrading culture wastewater containing high-concentration antibiotics and having low C/N ratio
  • Preparation method and application of phycomycete symbiont for degrading culture wastewater containing high-concentration antibiotics and having low C/N ratio

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specific Embodiment approach 1

[0038] Embodiment 1: A method for degrading algae-bacteria symbionts containing high-concentration antibiotics and low C / N ratio aquaculture wastewater according to this embodiment is carried out in the following steps:

[0039] Step 1: Add Chlorella pyrenoidosa to BG-11 medium, then pre-cultivate it in a photobioreactor, and cultivate it to the logarithmic phase, which is the OD on the fourth day 680 The range is 6-7, and the chlorella liquid is obtained; the parameters for pre-cultivation in the photobioreactor are: the culture temperature is 30°C, the culture speed is 200rpm, and the culture light is 200μmol / m 2 / s, the aeration volume is 1.5L / min; the aeration gas is CO 2 Mixed gas with air, the mixed gas is CO 2 The mass fraction is 2%; wherein the composition of the BG-11 medium is shown in Table 1;

[0040] Table 1 BG-11 medium composition

[0041]

[0042]Step 2: discard the supernatant after centrifuging the chlorella liquid obtained in step 1 at 8000rpm for 5mi...

specific Embodiment approach 2

[0049] Specific embodiment two: the algae-bacteria symbiont that degrades the aquaculture wastewater containing high concentrations of antibiotics and low C / N ratio in embodiment one is used to treat aquaculture wastewater containing high concentrations of antibiotics and low C / N ratio. The specific treatment method is as follows:

[0050] Inoculate the algae-bacteria symbiont of the specific embodiment 1 into the culture wastewater to be treated, at a culture temperature of 29-32°C, a culture speed of 200rpm-300rpm, and a culture light of 150μmol / m 2 / s~300μmol / m 2 / s, and the aeration rate is 1L / min~2L / min, and the pH value of the water body is adjusted to neutral every 12h from the inoculation;

[0051] Detection of NH by UV absorption method 4 + -N, get as figure 2 The algal symbiont to NH 4 + -N degradation curve graph from figure 2 It can be seen that on the second day of cultivation, compared with 10:1 and 1:1, the symbiotic system with an algae-to-bacteria rati...

Embodiment 1

[0052] Embodiment 1: A kind of preparation method of degrading algae-bacteria symbiosis containing high-concentration antibiotic and low C / N ratio culture wastewater of this embodiment is carried out according to the following steps:

[0053] Step 1: Add Chlorella pyrenoidosa to BG-11 medium, then pre-cultivate it in a photobioreactor, and cultivate it to the logarithmic phase, which is the OD on the fourth day 680 The range is 6.627, and the chlorella liquid is obtained; the parameters for pre-cultivation in the photobioreactor are: the culture temperature is 30°C, the culture speed is 200rpm, and the culture light is 200μmol / m 2 / s, the aeration volume is 1.5L / min; the aeration gas is CO 2 Mixed gas with air, the mixed gas is CO 2 The mass fraction is 2%; wherein the composition of the BG-11 medium is shown in Table 1;

[0054] Step 2: discard the supernatant after centrifuging the chlorella liquid obtained in step 1 at 8000rpm for 5min, wash the algae cell pellet obtained...

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Abstract

The invention relates to a preparation method and application of a phycomycete symbiont for degrading culture wastewater containing high-concentration antibiotics and having a low C/N ratio. The invention belongs to the field of water treatment, and aims to solve the technical problems of long treatment time, high cost and poor treatment effect caused by the fact that the existing process for biologically treating aquaculture wastewater by using activated sludge is complicated and all treatment processes cannot be synchronously carried out. The method comprises the following steps: culturing chlorella to a logarithmic phase, and centrifuging to obtain a chlorella cell precipitate; performing specific domestication on the activated sludge, and centrifuging to obtain activated sludge precipitate; and uniformly mixing the phycomycete and the phycomycete to obtain the phycomycete symbiont for degrading culture wastewater containing high-concentration antibiotics and having a low C/N ratio. According to the application, the obtained phycomycete symbiont is used for treating culture wastewater containing high-concentration antibiotics and having a low C/N ratio. The algae-bacteria symbiotic system can achieve a good nitrogen and phosphorus removal effect in a short time, is simple and easy to operate and relatively low in preparation cost, and has the potential of large-scale popularization and application.

Description

technical field [0001] The invention belongs to the field of water treatment, and in particular relates to a preparation method and application of algae-bacteria symbiosis that degrades aquaculture wastewater containing high concentrations of antibiotics and low C / N ratio. Background technique [0002] my country is a big country of livestock and poultry breeding, which produces a large amount of livestock and poultry wastewater every year. The water contains a large amount of organic matter (COD), nitrogen, phosphorus, veterinary drug residues, etc., with complex components, high pollution load, and foul odor. It is a high-concentration organic wastewater. After livestock and poultry breeding water is discharged into the surrounding water body, a large amount of oxygen in the water will be consumed, resulting in the death of aquatic organisms, and the water body will stink; the high concentration of nitrogen and phosphorus contained in the wastewater will also cause eutroph...

Claims

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Application Information

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IPC IPC(8): C02F3/32C02F3/34C02F101/30C02F103/20
CPCC02F3/325C02F3/34C02F2103/20C02F2101/30Y02W10/10
Inventor 贺诗欣李胜男张超凡
Owner HARBIN INST OF TECH
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