Polypeptide with function of promoting mineralization and regeneration of enamel structure
A structural and enamel technology, applied in the field of peptides that promote the structural mineralization and regeneration of enamel, can solve problems such as enamel cavities, and achieve the effects of promoting remineralization, clear effect and easy preparation
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Embodiment 1
[0027] Example 1, the design of a polypeptide with the function of promoting the structural mineralization and regeneration of enamel, the design principle is as follows:
[0028] Before the enamel matures, it needs to form a structurally ordered enamel matrix to induce hydroxyapatite to mineralize in a certain direction, thereby forming a specific crystal structure. The assembly mechanism of enamel matrix in vitro can be divided into two stages: (1) controlled aggregation reaction and (2) induced crystal growth in a specific direction as AMELX-Ca-P nanoclusters gradually form a hierarchical structure.
[0029] Human AMELX (H175) has a peptide chain length of 175 (recombinant is 174, also known as rH174), which is a bipolar molecule, most of which are hydrophobic and contain hydrophilic amino acid residues at the carboxy terminus. Some studies believe that the bipolarity of AMELX is the prerequisite for AMELX to self-assemble to form various nanostructures, and these nanostruc...
Embodiment 2
[0032] Example 2, Synthesis of Polypeptide, Determination of Physicochemical Properties and Morphological Observation
[0033] 1. Polypeptide synthesis
[0034] Peptides were synthesized chemically by Sangon Bioengineering (Shanghai) Co., Ltd. After freeze-drying the peptides, store them frozen at -20°C.
[0035] 2. Physicochemical properties of peptides (circular dichroism analysis)
[0036] 1. Purpose of the experiment
[0037] The possible secondary structure of the polypeptide is determined by measuring the absorbance of the circular dichroism spectrum of the polypeptide.
[0038] 2. Experimental instruments, reagents and methods
[0039] The lyophilized peptide powder was thawed at 25°C for 20 minutes, then dissolved in double-distilled deionized water, and the volume was adjusted to 1mg / mL as a storage mother solution, and placed in a 37°C constant temperature water bath for 1 hour. Using Bio-Logic MOS-500 circular dichroism (CD) instrument, under the condition of 2...
Embodiment 3
[0050] Example 3, the remineralization ability of polypeptides to acid-etched enamel
[0051] 1. Preparation of acid-etched enamel samples
[0052] Utilize the MTI SYJ-150 low-speed diamond sawing machine to cut the bovine central incisor into a cuboid of 5mm*5mm*3mm (length*width*height). Use 180, 320, 600, 1200, 2500 mesh sandpaper in turn to grind evenly, then use a large amount of double distilled water to rinse and use Shumei KQ3200DB CNC ultrasonic cleaning machine to ultrasonically clean. Apply 37% phosphoric acid etchant evenly on the cleaned and dried enamel sample and etch for 60 seconds, then rinse with a sufficient amount of double distilled water. Soak the acid-etched enamel samples in double distilled water and refrigerate at 4°C for later use.
[0053] 2. Preparation of Enamel Remineralization Solution
[0054] Use double-distilled water to dissolve potassium dihydrogen phosphate, calcium chloride, and sodium chloride, add a small amount of sodium pyrophospha...
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