Maize southern rust resistance gene and its application

A technology for southern rust and corn, which is applied in the field of corn southern rust resistance gene and its application, and can solve the problem of single source of resistance

Active Publication Date: 2022-07-12
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in the process of maize breeding in my country, most of the resistance source materials used for southern rust are from tropical inbred lines, and the resistance source is too single

Method used

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  • Maize southern rust resistance gene and its application
  • Maize southern rust resistance gene and its application
  • Maize southern rust resistance gene and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1. Discovery of resistance genes

[0062] Based on corn inbred lines Jing 2416 and Jing 2416K, configuration F 1 Hybrid, the F 1 Obtained F of Jing 2416×Jing 2416K on behalf of self-introduction 2 Populations were segregated for resistance gene mapping. All experimental materials were planted in Sanya, Hainan, a high incidence area of ​​southern corn rust. Sow mid-October, F 2 The population identification materials were randomly arranged and planted in a single row. Each material was sown with a row length of 5m and a row spacing of 0.55m, with 20 to 25 seedlings in each row. Maize inbred lines Jing 2416K and Jing 2416 were set up in a group of high resistance and high sensitivity control materials in every 50 rows. Field management is carried out according to general field production.

[0063] Resistance identification was carried out in Sanya, Hainan, relying on natural disease. Surveys were conducted from the milk to wax stage of corn (approximately 1...

Embodiment 2

[0081] Example 2. Application of resistance gene in maize genetic improvement

[0082] 1. Preparation of transgenic corn

[0083] 1. Construction of recombinant vector

[0084] 1) The pYBA-p1132 vector was digested with restriction endonuclease EcoRI to obtain the linearized pYBA-p1132 vector, and the linearized vector was recovered from agarose gel.

[0085] The pYBA-p1132 vector is a vector obtained by inserting an expression cassette of a selectable marker gene (herbicide resistance gene Bar gene) at position 1369 of the pYBA vector. The nucleotide sequence of the expression cassette of the selectable marker gene (herbicide resistance gene Bar gene) is shown in SEQ ID NO: 5, which sequentially includes 35S promoter, selectable marker gene Bar and NOS terminator.

[0086] 2) Using the cDNA of Jing 2416K as a template, PCR amplification was carried out with primers pYBA-65F / R and pYBA-67F / R, respectively, and the full-length fragment of the coding region of Zm00001d023265 g...

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Abstract

The invention discloses a maize southern rust resistance gene and its application. The present invention utilizes the F of the inbred line Jing 2416 and Jing 2416K 2 Genetic analysis of the isolated population showed that the resistance gene contained in Jing 2416K was a single dominant gene, named RppM. Through BSA resequencing analysis and genetic linkage analysis, RppM was located in the 110-kb interval of the short arm of maize chromosome 10, and the candidate genes in this interval were subjected to functional annotation analysis, sequence alignment analysis and expression analysis to determine Zm00001d023265 and Zm00001d023267 are candidate genes of RppM, and molecular cloning of the two genes was carried out to obtain the coding region sequences of the two genes. The transgenic experiments proved that both genes have the function of improving the resistance of maize to southern rust, providing new resistance materials and gene resources for maize resistance breeding.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a maize southern rust resistance gene and its application. Background technique [0002] Corn is the grain and cash crop with the largest planting area and the highest total output in my country, and occupies an important position in the national economy. Maize disease is an important factor affecting yield. With global climate change, changes in farming methods and the promotion of new varieties, the incidence of corn diseases in my country has also changed. pose a great threat. Maize southern rust is a fungal disease caused by Puccinia polysora Underw., mainly distributed in tropical and subtropical regions, and is a very harmful airborne disease. In recent years, the disease has a tendency to spread to the north of my country. Once it breaks out and becomes popular, it generally causes a 20-30% yield reduction, and in severe cases it can be as high as 80% o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8282
Inventor 王帅赵久然宋伟张如养苏爱国王元东邢锦丰王帅帅
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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