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A kind of bovine lactoferrin peptide chitosan nanoparticle and its preparation method and a kind of freeze-dried powder

A bovine lactoferrin peptide and chitosan nanoparticle technology, applied in the field of nanomaterials, can solve problems such as inability to achieve ideal effects, and achieve the effects of maintaining bacteriostatic effect, good biocompatibility, and promoting proliferation

Active Publication Date: 2022-08-09
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although AMPs have many biological functions, intravenous or oral administration cannot achieve the desired effect, which is likely due to the inactivation of AMPs by serum proteins after intravenous injection or enzymatic degradation in the gastrointestinal tract after oral administration.

Method used

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  • A kind of bovine lactoferrin peptide chitosan nanoparticle and its preparation method and a kind of freeze-dried powder
  • A kind of bovine lactoferrin peptide chitosan nanoparticle and its preparation method and a kind of freeze-dried powder
  • A kind of bovine lactoferrin peptide chitosan nanoparticle and its preparation method and a kind of freeze-dried powder

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preparation example Construction

[0030] The invention provides a preparation method of bovine lactoferrin peptide chitosan nanoparticles, comprising the following steps:

[0031] 1) dissolving the low molecular weight chitosan in the glacial acetic acid solution, adjusting the pH of the solution to 3-5, to obtain a low molecular weight chitosan solution;

[0032] 2) adding bovine lactoferrin peptide to the low molecular weight chitosan solution and reacting to obtain reaction solution A;

[0033] 3) The sodium tripolyphosphate solution is added to the reaction solution A, and the reaction is continued until a light blue opalescent dispersion is obtained.

[0034] In the present invention, the volume concentration of the glacial acetic acid solution is 0.01-0.5%, preferably 0.05-0.4%, more preferably 0.1-0.3%, and more preferably 0.2%.

[0035] In the present invention, the concentration of the low molecular weight chitosan solution is 0.5-2 mg / mL, preferably 0.6-1.8 mg / mL, more preferably 0.8-1.5 mg / mL, more...

Embodiment 1

[0049] Preparation of bovine lactoferrin-peptide-chitosan nanoparticles:

[0050] Dissolve low-molecular-weight chitosan in 0.2% glacial acetic acid solution to make a 1 mg / ml solution, adjust its pH to 4.5 with 1 mol / L sodium hydroxide solution, and autoclave for later use; Sodium tripolyphosphate was dissolved in pure water to prepare a solution with a concentration of 1 mg / ml, which was filtered and sterilized through a 0.22 μm disposable needle filter, and then placed in a refrigerator for later use. The low-molecular-weight chitosan solution was preheated in a 60°C water bath for 10 minutes. Under the condition of magnetic stirring, a certain amount of bovine lactoferrin peptide was added to mix evenly, and then the sodium tripolyphosphate solution was added dropwise at a constant speed, and stirred for 30 minutes. The bovine lactoferrin peptide-loaded chitosan nanoparticle with light blue opalescence, wherein the mass-volume ratio of bovine lactoferrin peptide, sodium tr...

Embodiment 2

[0054] Preparation of bovine lactoferrin-peptide-chitosan nanoparticles:

[0055] Dissolve low molecular weight chitosan in 0.5% glacial acetic acid solution to make a solution of 0.8 mg / ml, adjust its pH value to 4.0 with 1.2 mol / L sodium hydroxide solution, and use it after autoclaving. Dissolve sodium tripolyphosphate in pure water to prepare a solution with a concentration of 0.8 mg / ml, filter and sterilize it through a 0.22 μm disposable needle filter, and then place it in a refrigerator for later use. The low molecular weight chitosan solution was preheated in a 55°C water bath for 15 minutes, and under magnetic stirring, a certain amount of bovine lactoferrin peptide was added to mix evenly, and then the sodium tripolyphosphate solution was added dropwise at a constant rate, and stirred for 30 minutes. The bovine lactoferrin peptide-loaded chitosan nanoparticles with light blue opalescence, wherein the mass-volume ratio of bovine lactoferrin peptide, sodium tripolyphosp...

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Abstract

The invention provides a preparation of bovine lactoferrin peptide chitosan nanoparticles, a preparation method and a freeze-dried powder, belonging to the technical field of nanomaterials. In the present invention, the low molecular weight chitosan powder is dissolved in the glacial acetic acid solution, and the pH of the solution is adjusted to 3-5 to obtain the low molecular weight chitosan solution; the bovine lactoferrin peptide solution is added to the low molecular weight chitosan solution to react to obtain a reaction Solution A; add sodium tripolyphosphate solution to reaction solution A and continue to react until a light blue opalescent dispersion is obtained. The bovine lactoferrin peptide-loaded chitosan nanoparticle prepared by the invention has good physicochemical characterization and in vitro antibacterial effect, has no cytotoxicity, and can be used as a promising antimicrobial peptide drug release system.

Description

technical field [0001] The invention relates to the technical field of nanomaterials, in particular to a bovine lactoferrin peptide chitosan nanoparticle, a preparation method thereof and a freeze-dried powder. Background technique [0002] Antimicrobial peptides (AMPs) produced by most multicellular organisms serve as a rapid first line of defense against invading pathogenic microorganisms, which enables multicellular organisms to survive in a competitive environment without the need for a complex adaptive immune system. AMPs are composed of amino acids, come in different sizes and shapes, usually carry a net positive charge, and have an amphiphilic structure. The mechanisms of antibacterial action of different classes of AMPs vary, but generally AMPs can directly interact with negatively charged bacterial membranes, causing membrane depolarization. AMPs are ubiquitous in organisms, and they have gained considerable attention in antibacterial in recent years, and are consi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/40A61K9/51A61K9/19A61K47/36A61K47/26A61P15/14A61P31/04
CPCA61K38/40A61K9/5161A61K9/19A61K47/36A61K47/26A61P15/14A61P31/04
Inventor 童津津张华崔德凤蒋林树
Owner BEIJING UNIV OF AGRI
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