Nucleic acid extractor

Abstract

The invention discloses a nucleic acid extractor, wherein the driving assembly includes a magnetic rod assembly and a magnetic sleeve assembly, the magnetic rod assembly and the magnetic sleeve assembly are arranged above the heating assembly, the magnetic sleeve assembly includes a magnetic rod sleeve, and the magnetic rod sleeve is connected to There is a magnetic sleeve drive structure, the magnetic sleeve drive structure is directly connected with the second drive assembly, and the second drive assembly directly drives the magnetic sleeve drive structure to move; the magnetic rod assembly includes a magnetic rod, and the magnetic rod is connected to a magnetic rod carrying structure, and the magnetic rod carries A driving arm protrudes from the structure, and a magnetic rod driving structure is arranged under the driving arm. The magnetic rod driving structure is directly connected with the first driving assembly, and the surface of the magnetic rod driving structure is contact-connected with the surface of the driving arm. The first The driving assembly drives the magnetic rod driving structure to move upwards, indirectly drives the driving arm to drive the magnetic rod carrying structure to move upward, and the magnetic rod carrying structure moves downwards relying on the gravity of the magnetic rod assembly itself. The driving effect of the nucleic acid extraction instrument of the present invention is safe, reliable and not easy to be damaged, small in size and high in heating efficiency.

Description

technical field [0001] The invention relates to the field of genetic engineering instruments and equipment, in particular to a nucleic acid extractor. Background technique [0002] Nucleic acid extraction instrument is an instrument that uses supporting nucleic acid extraction reagents to automatically complete sample nucleic acid extraction. It is widely used in disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial detection, food safety testing, animal husbandry and molecular biology. various fields of research. Nucleic acid extraction includes the following basic steps: 1. Cleavage: add lysate to the sample, and through mechanical movement and heating, the reaction solution is mixed and fully reacted to lyse the cells and release nucleic acid; 2. Adsorption: lyse the sample Add magnetic beads to the solution, mix well, use the magnetic beads to have a strong affinity for nucleic acid under high sal...

AI Technical Summary

Problems solved by technology

However, there are some problems in this driving method. Since the magnetic rod and the magnetic rod sleeve need to have precise motion coordination in each step, once the control system or detection system of the instrument fails, or the instrument runs for a long time, the motion mechanism will Poor movement accuracy may cause the magnetic rod to move excessively towards the magnetic rod sleeve, collide with each other, and damage the instrument or even cause an accident

Especially when the magnetic rod and the magnetic rod sleeve need to move or vibrate together, the driving of the two sets of power elements needs to be kept absolutely synchronized, which not only makes the hardware and software design of the instrument more difficult and difficult to implement, but also makes the magnetic rod more likely to occur. Problems with excessive movement relative to the ferrule sleeve, creating a hazard

[0005] In addition, due to the large difference in the amount of reagents used in the two steps of cleavage and elution, the heating requirements of the two steps are different, while the existing heating device uses the same heating structure and heating method for the cleavage and elution steps , which leads to the obvious insufficient heating effect of the heating device for the cleavage step, and the obvious excess heating effect of the heating device for the elution step, thus causing the problems of low heating efficiency and slow nucleic acid extraction speed

Due to the long heating time required for the cracking step, the heat in the cracking area will radiate more to the experimental areas such as washing and elution next to it, which will affect the experimental results of other steps; Smaller, the heating capacity of the heating device is too large to cause excessive volatilization of the eluent, waste reagents, and affect the concentration of the reagents. Even during the oscillation process, the magnetic beads are heated and evaporated to dryness on the inner wall of the test tube, so that the nucleic acid on the magnetic beads cannot be dissolved normally. In the eluent, affect the sample quality

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