Mutant of threonine aldolase and application thereof in preparation of substituted phenylserine derivatives

A threonine aldolase, mutant technology, applied in application, biochemical equipment and methods, introduction of foreign genetic material using vectors, etc. Acid aldolase has problems such as poor stability, and achieves excellent application prospects and good stability.
CN113481188AActive Publication Date: 2021-10-08王喆明 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
王喆明
Publication Date
2021-10-08

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Abstract

The invention discloses a mutant of threonine aldolase, a coding gene of the mutant, a recombinant vector constructed by the coding gene, a recombinant genetic engineering bacterium obtained by converting the recombinant vector, and application of the mutant of the threonine aldolase in preparation of 2- / 3- / 4-substituted phenylserine derivatives. According to the invention, the mutant of the threonine aldolase is taken as a biocatalyst, 2- / 3- / 4-substituted benzaldehyde is taken as a substrate, 5-phosphopyridoxal is taken as a coenzyme, glycine / glycine ester is taken as a cosubstrate, the enzyme catalysis reaction is performed under proper conditions and in a proper medium, a series of phenylserine derivatives with different substituent groups are prepared through separation, and the method has the total yield range of 76-99%, the product ee value of over 99%, and the de value range of 71-92%.
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Description

[0001] This application is a divisional application with application number 201811310893.4, application date November 6, 2018, and invention title "threonine aldolase, mutants and their application in the preparation of substituted phenylserine derivatives". technical field

[0002] The invention relates to the field of biotechnology, in particular to a threonine aldolase mutant and its application in the preparation of 2- / 3- / 4-position substituted phenylserine derivatives. Background technique

[0003] 2- / 3- / 4-substituted phenylserine derivatives are important pharmaceutical intermediates and chemical intermediates, especially 4-thiamphenicol phenylserine, which are widely used in the preparation of human antibiotics, poultry and livestock antibiotics, etc. , which represent drugs such as chloramphenicol, thiamphenicol, florfenicol and so on.

[0004] The synthesis methods of 2- / 3- / 4-position substituted phenylserine derivatives mainly include chemical synthesis and enzyme ...

Claims

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