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Bioelectrochemical method for synchronously detecting nitrite and biochemical oxygen demand

A bioelectrochemical and nitrite technology is applied in the field of bioelectrochemistry for simultaneous detection of nitrite and biochemical oxygen demand, which can solve the problem of high detection cost, and achieve the effects of improving accuracy and reducing device cost.

Active Publication Date: 2021-10-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, when using bioelectrochemical sensor technology to detect different redox substances, it is often necessary to construct different bioelectrochemical sensor devices, which makes the detection cost high when using bioelectrochemical sensor technology to detect sewage containing various pollutants.

Method used

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  • Bioelectrochemical method for synchronously detecting nitrite and biochemical oxygen demand
  • Bioelectrochemical method for synchronously detecting nitrite and biochemical oxygen demand
  • Bioelectrochemical method for synchronously detecting nitrite and biochemical oxygen demand

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Use the sludge water in the anoxic section of the sewage plant as the inoculum, and cultivate the bioelectrode for the bidirectional detector in the MFC mode (that is, the microbial fuel cell mode):

[0071] The resistance 18 externally connected to the bioelectrochemical reactor is connected with a copper wire for the loop. The bioelectrochemical reactor was inoculated with the sludge water in the anoxic section. The inoculation process lasted for five days, and then it was transferred to the sequence batch mode for cultivation, and the matrix solution was replaced every day. The voltage of the MFC was recorded by a digital data collection instrument (KEYSIGHT model 34970A).

[0072] Specifically: the biological electrode 12 in the bioelectrochemical reactor is used as an anode, the non-biological electrode 13 is used as a negative electrode externally connected to a resistor 18 and a copper wire to form a loop, such as Pic 4-1 As shown, the bioelectrochemical reacto...

Embodiment 2

[0077] The bioelectrode is tested with CV in different solutions, and the most suitable detection potential of BOD and nitrite in the process of potential step chronoamperometry is selected according to the test results:

[0078] The bioelectrochemical reactor is connected with the potentiostat 19 with a three-electrode system to form a bioelectrochemical sensor, wherein graphite electrodes (bioelectrodes), nickel foam air electrodes and silver / silver chloride electrodes are used as working electrodes respectively, counter electrode and reference electrode. Place the bioelectrodes in 50mM PBS, add 100mgBOD L -1 Sodium acetate in 50mM PBS, and add 20mgNO 2 - -NL -1 CV tests were performed in nitrite in 50 mM PBS. The scanning range of CV is -0.6V~-0.1V (vs.Ag / AgCl), and the scanning speed is 0.5mV s -1 . Among them, in the follow-up testing process of the constructed bioelectrochemical sensor (including the test of the solution containing a single pollutant and the mixed ...

Embodiment 3

[0081] The bioelectrode performs potentiometric step chronoamperometric tests in solutions containing different nitrite concentrations and solutions with different BOD concentrations, and the signal output time point is selected according to the test results:

[0082] The bioelectrochemical reactor was connected to a potentiostat with a three-electrode system, and graphite electrodes (bioelectrodes), nickel foam air electrodes and silver / silver chloride electrodes were used as working electrodes, counter electrodes and reference electrodes, respectively. By adding 12.5mL L -1 Trace minerals and 5mL L -1 Add nitrite to vitamin 50mMPBS to prepare a standard nitrite-containing solution, and set the concentration of the standard nitrite solution to 2mgNO 2 - -N L -1 ,4mgNO 2 - -N L -1 ,8mgNO 2 - -N L -1 , 12mgNO 2 - -N L -1 and 16mgNO 2 - -N L -1 . By adding 12.5mL L -1 Trace minerals and 5mL L -1 Add sodium acetate to 50mMPBS of vitamins to prepare a standard so...

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Abstract

The invention discloses a bioelectrochemical method for synchronously detecting nitrite and biochemical oxygen demand, which comprises the following steps: 1) operating a bioelectrochemical reactor in a closed circuit in an MFC mode, and adding an inoculation solution into an anode chamber; culturing the biological electrode in the matrix solution until the biological electrode is mature; 2) connecting the bioelectrochemical reactor with a constant potential rectifier to construct a bioelectrochemical sensor, performing cyclic voltammetry test on a bioelectrode in a nitrite-containing solution and a BOD organic matter-containing solution respectively, and selecting BOD organic matter and nitrite detection potentials in a potential step timing current test process; (3) carrying out potential step timing current testing on the biological electrode in solutions with different concentrations of nitrite and BOD organic matters, and selecting a signal output time point to obtain detection marking lines of electric response and the concentrations of the nitrite and the BOD organic matters; and according to the electrical response of the bioelectrochemical sensor in the sewage containing nitrite or the sewage containing BOD organic matters, respectively obtaining the concentrations of the nitrite and the BOD organic matters in the sewage.

Description

technical field [0001] The invention relates to a pollutant detection technology based on a bioelectrochemical reaction, which belongs to the field of biosensor and water body pollutant detection. The bioelectrochemical detection method is applied to a bioelectrochemical sensor, which can realize synchronous detection of nitrite and biochemical in sewage. Oxygen demand (BOD), specifically relates to a bioelectrochemical method for simultaneous detection of nitrite and biochemical oxygen demand. Background technique [0002] With the development of urbanization, the impact of urban sewage discharge on the environment continues to intensify, and the treatment and detection of urban sewage has become a hot issue of concern. Two typical pollutants, nitrite and organic matter, are often contained in urban sewage. Among them, nitrite is likely to cause eutrophication of natural water bodies and is one of the important causes of diseases such as cancer and hypertension. Organic m...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/327G01N27/30
CPCG01N27/48G01N27/327G01N27/308
Inventor 成少安林朱凡
Owner ZHEJIANG UNIV