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Poly (N-vinylcaprolactam) nanogel loaded with gold and manganese dioxide nanoparticles as well as preparation and application of poly (N-vinylcaprolactam) nanogel

A technology of vinyl caprolactam and gold nanoparticles, which can be used in preparations for in vivo tests, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc., and can solve the problems of low radiotherapy sensitivity of nano-diagnostic agents , to achieve good colloidal stability and cytocompatibility, good development and application prospects, and high r1 relaxation rate.

Active Publication Date: 2021-10-15
DONGHUA UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a poly N-vinyl caprolactam nanogel loaded with gold and manganese dioxide nanoparticles and its preparation method and application , to overcome the defects of low radiosensitivity of nano-therapeutic agents in the prior art

Method used

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  • Poly (N-vinylcaprolactam) nanogel loaded with gold and manganese dioxide nanoparticles as well as preparation and application of poly (N-vinylcaprolactam) nanogel
  • Poly (N-vinylcaprolactam) nanogel loaded with gold and manganese dioxide nanoparticles as well as preparation and application of poly (N-vinylcaprolactam) nanogel
  • Poly (N-vinylcaprolactam) nanogel loaded with gold and manganese dioxide nanoparticles as well as preparation and application of poly (N-vinylcaprolactam) nanogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] (1) Dissolve 469.5mg of N-vinylcaprolactam (VCL), 14.5mg of N,N-methylenebisacrylamide (BIS) and 8.8mg of sodium lauryl sulfate in 30mL of ultrapure water deoxygenated in advance , to which was added 3 mL of acetoacetate methacrylate in water (AAEM, 14 mg / mL), in N 2 Under ambient conditions, stir at 70°C for 30 min. Then, 1.5 mL of an aqueous solution of azodicarboxyethyl-2-isobutylamidine (ACMA, 11.7 mg / mL) was added to the above solution. After stirring and reacting for 5 min, 3 mL of an aqueous solution of AAEM (14 mg / mL) was added to the solution, and stirring was continued for 4 h. Afterwards, the reacted solution was dialyzed in an 8-14kDa dialysis bag for 3 days, and freeze-dried to obtain poly N-vinyl caprolactam nanogel PVCL NGs.

[0079] (2) Disperse the PVCL NGs (100 mg) prepared in step (1) in 18 mL of ultrapure water, then add 1 mL of chloroauric acid aqueous solution (15 mg / mL) dropwise, stir in an ice bath for 30 min, and then quickly add 2 mL of boron...

Embodiment 2

[0082] Get the PVCL NGs, PVCL-Au NGs and PVCL-Au-MnO that embodiment 1 prepares 2 NGs solution (500 μg / mL) was used to measure surface potential and hydrodynamic diameter. The measurement results( figure 2 ) showed that the surface potential of PVCL NGs was −5.31 mV and the hydrodynamic diameter was 207.2 nm. After loading gold nanoparticles, the surface potential changed to −7.25 mV, and the hydrodynamic diameter changed to 215.3 nm, indicating the successful loading of gold nanoparticles. After further in-situ loading of manganese dioxide nanoparticles, the surface potential changed to −9.73 mV, and the hydrodynamic diameter changed to 220.7 nm, indicating the successful loading of manganese dioxide nanoparticles. Meanwhile, PVCLNGs, PVCL-Au NGs and PVCL-Au-MnO 2 After NGs were stored in water for up to 30 days, the size did not change significantly ( image 3 ), indicating the good colloidal stability of the nanogels. Afterwards, measured the PVCL NGs and PVCL-Au-Mn...

Embodiment 3

[0084] The PVCL NGs, PVCL-Au NGs and PVCL-Au-MnO 2 NGs were dispersed in water (1 mg / mL), and the pH was adjusted to 6.5. Subsequently, H was added to each solution 2 o 2 , such that H 2 o 2 The concentration is 100×10 -6 M, then record the change of oxygen in each solution with a dissolved oxygen meter. See attached Figure 7 , compared with the control group (H alone 2 o 2 ) compared to PVCL-Au-MnO 2 The dissolved oxygen concentration in the NGs group was significantly increased, indicating that the PVCL-Au-MnO synthesized by the present invention 2 NGs can well catalyze H under acidic conditions 2 o 2 , and produce oxygen. In contrast, the oxygen concentration in the PVCL NGs and PVCL-Au NGs groups did not change significantly, indicating that PVCLNGs and PVCL-Au NGs could not catalyze H 2 o 2 Oxygen is produced.

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Abstract

The invention relates to poly (N-vinylcaprolactam) nanogel loaded with gold and manganese dioxide nanoparticles as well as a preparation method and application of the poly (N-vinylcaprolactam) nanogel. The nanogel is prepared from gold nanoparticles, manganese dioxide nanoparticles, acetoacetic acid ethylene glycol methacrylate and poly (N-vinyl caprolactam) nanogel. The method comprises the following steps: preparing poly (N-vinylcaprolactam) nanogel, preparing poly (N-vinylcaprolactam) nanogel loaded with gold nanoparticles, and preparing poly (N-vinylcaprolactam) nanogel loaded with gold and manganese dioxide nanoparticles. The method is simple in process, easy to operate and separate, wide in raw material source and good in development and application prospect; the prepared nanogel has the advantages of good colloidal stability, cell compatibility, good X-ray attenuation, high r1 relaxation rate and good'whole process' radiotherapy sensitization.

Description

technical field [0001] The invention belongs to the field of nanometer diagnosis and treatment agent and its preparation and application, in particular to a poly N-vinyl caprolactam nanogel loaded with gold and manganese dioxide nanoparticles and its preparation method and application. Background technique [0002] With the increasing pollution of human living environment, malignant tumor (cancer) has become one of the public health problems that seriously threaten human health. Radiotherapy is a method of local tumor treatment using radiation, and it is still an important local treatment method for malignant tumors until now. According to statistics, about 70% of cancer patients need radiotherapy in different stages of cancer treatment. However, in the course of clinical treatment, its curative effect is often limited by the radiation dose. Too low radiation dose may lead to incomplete eradication of the tumor and lead to tumor recurrence or metastasis, while too high radi...

Claims

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Application Information

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IPC IPC(8): A61K49/12A61K49/18A61K49/04A61K49/00A61K41/00A61K9/06A61K47/32A61P35/00
CPCA61K49/126A61K49/1803A61K49/04A61K49/0002A61K41/0057A61K9/06A61K47/32A61P35/00
Inventor 史向阳刘勇张昌昌涂文志
Owner DONGHUA UNIV
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